Selection of candidate genes affecting meat quality and preliminary exploration of related molecular mechanisms in the Mashen pig
Objective The aim of this study was to select the candidate genes affecting meat quality and preliminarily explore the related molecular mechanisms in the Mashen pig. Methods The present study explored genetic factors affecting meat quality in the Mashen pig using RNA sequencing (RNA-Seq). We sequen...
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Asian-Australasian Association of Animal Production Societies
2019-08-01
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doaj-edc337b2288041508c9cc5cdf1136a9e2020-11-25T01:09:21ZengAsian-Australasian Association of Animal Production SocietiesAsian-Australasian Journal of Animal Sciences1011-23671976-55172019-08-013281084109410.5713/ajas.18.071824227Selection of candidate genes affecting meat quality and preliminary exploration of related molecular mechanisms in the Mashen pigPengfei Gao0Zhimin Cheng1Meng Li2Ningfang Zhang3Baoyu Le4Wanfeng Zhang5Pengkang Song6Xiaohong Guo7Bugao Li8Guoqing Cao9 College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, China College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu 030801, ChinaObjective The aim of this study was to select the candidate genes affecting meat quality and preliminarily explore the related molecular mechanisms in the Mashen pig. Methods The present study explored genetic factors affecting meat quality in the Mashen pig using RNA sequencing (RNA-Seq). We sequenced the transcriptomes of 180-day-old Mashen and Large White pigs using longissimus dorsi to select differentially expressed genes (DEGs). Results The results indicated that a total of 425 genes were differentially expressed between Mashen and Large White pigs. A gene ontology enrichment analysis revealed that DEGs were mainly enriched for biological processes associated with metabolism and muscle development, while a Kyoto encyclopedia of genes and genomes analysis showed that DEGs mainly participated in signaling pathways associated with amino acid metabolism, fatty acid metabolism, and skeletal muscle differentiation. A MCODE analysis of the protein-protein interaction network indicated that the four identified subsets of genes were mainly associated with translational initiation, skeletal muscle differentiation, amino acid metabolism, and oxidative phosphorylation pathways. Conclusion Based on the analysis results, we selected glutamic-oxaloacetic transaminase 1, malate dehydrogenase 1, pyruvate dehydrogenase 1, pyruvate dehydrogenase kinase 4, and activator protein-1 as candidate genes affecting meat quality in pigs. A discussion of the related molecular mechanisms is provided to offer a theoretical basis for future studies on the improvement of meat quality in pigs.http://www.ajas.info/upload/pdf/ajas-18-0718.pdfPigCandidate GeneMolecular MechanismMeat QualityRNA-Seq |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pengfei Gao Zhimin Cheng Meng Li Ningfang Zhang Baoyu Le Wanfeng Zhang Pengkang Song Xiaohong Guo Bugao Li Guoqing Cao |
spellingShingle |
Pengfei Gao Zhimin Cheng Meng Li Ningfang Zhang Baoyu Le Wanfeng Zhang Pengkang Song Xiaohong Guo Bugao Li Guoqing Cao Selection of candidate genes affecting meat quality and preliminary exploration of related molecular mechanisms in the Mashen pig Asian-Australasian Journal of Animal Sciences Pig Candidate Gene Molecular Mechanism Meat Quality RNA-Seq |
author_facet |
Pengfei Gao Zhimin Cheng Meng Li Ningfang Zhang Baoyu Le Wanfeng Zhang Pengkang Song Xiaohong Guo Bugao Li Guoqing Cao |
author_sort |
Pengfei Gao |
title |
Selection of candidate genes affecting meat quality and preliminary exploration of related molecular mechanisms in the Mashen pig |
title_short |
Selection of candidate genes affecting meat quality and preliminary exploration of related molecular mechanisms in the Mashen pig |
title_full |
Selection of candidate genes affecting meat quality and preliminary exploration of related molecular mechanisms in the Mashen pig |
title_fullStr |
Selection of candidate genes affecting meat quality and preliminary exploration of related molecular mechanisms in the Mashen pig |
title_full_unstemmed |
Selection of candidate genes affecting meat quality and preliminary exploration of related molecular mechanisms in the Mashen pig |
title_sort |
selection of candidate genes affecting meat quality and preliminary exploration of related molecular mechanisms in the mashen pig |
publisher |
Asian-Australasian Association of Animal Production Societies |
series |
Asian-Australasian Journal of Animal Sciences |
issn |
1011-2367 1976-5517 |
publishDate |
2019-08-01 |
description |
Objective The aim of this study was to select the candidate genes affecting meat quality and preliminarily explore the related molecular mechanisms in the Mashen pig. Methods The present study explored genetic factors affecting meat quality in the Mashen pig using RNA sequencing (RNA-Seq). We sequenced the transcriptomes of 180-day-old Mashen and Large White pigs using longissimus dorsi to select differentially expressed genes (DEGs). Results The results indicated that a total of 425 genes were differentially expressed between Mashen and Large White pigs. A gene ontology enrichment analysis revealed that DEGs were mainly enriched for biological processes associated with metabolism and muscle development, while a Kyoto encyclopedia of genes and genomes analysis showed that DEGs mainly participated in signaling pathways associated with amino acid metabolism, fatty acid metabolism, and skeletal muscle differentiation. A MCODE analysis of the protein-protein interaction network indicated that the four identified subsets of genes were mainly associated with translational initiation, skeletal muscle differentiation, amino acid metabolism, and oxidative phosphorylation pathways. Conclusion Based on the analysis results, we selected glutamic-oxaloacetic transaminase 1, malate dehydrogenase 1, pyruvate dehydrogenase 1, pyruvate dehydrogenase kinase 4, and activator protein-1 as candidate genes affecting meat quality in pigs. A discussion of the related molecular mechanisms is provided to offer a theoretical basis for future studies on the improvement of meat quality in pigs. |
topic |
Pig Candidate Gene Molecular Mechanism Meat Quality RNA-Seq |
url |
http://www.ajas.info/upload/pdf/ajas-18-0718.pdf |
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