Ethyl pyruvate pretreatment attenuates concanavalin a-induced autoimmune hepatitis in mice.
Ethyl pyruvate (EP), a potent reactive oxygen species scavenger, has been reported to contribute to the inflammatory process. However, the protective effect of ethyl pyruvate on Concanavalin A (Con A)-induced autoimmune hepatitis have not been explored. Thus, the aims of this study are to investigat...
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doaj-edeccd38f14048a6b869cc302458c3db2020-11-25T00:47:14ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0192e8797710.1371/journal.pone.0087977Ethyl pyruvate pretreatment attenuates concanavalin a-induced autoimmune hepatitis in mice.Miao ShenJie LuPing ChengChunlei LinWeiqi DaiFan WangChengfen WangYan ZhangKan ChenLing XuYinqun ZhouChuanyong GuoEthyl pyruvate (EP), a potent reactive oxygen species scavenger, has been reported to contribute to the inflammatory process. However, the protective effect of ethyl pyruvate on Concanavalin A (Con A)-induced autoimmune hepatitis have not been explored. Thus, the aims of this study are to investigate both the effects of ethyl pyruvate and its mechanism of protection on Con A-induced autoimmune hepatitis in mice.Acute autoimmune hepatitis was induced by Con A (20 mg/kg) in Balb/C mice; ethyl pyruvate (40 mg/kg and 80 mg/kg) was administrated 1h prior to the Con A injection. At 3h, 6h and 24h post Con A injection, histological grading, proinflammatory cytokine levels and nuclear factor kappa B (NF-κB) activity were determined.Following Con A challenge, cytokines TNF-α, IL-2, IL-1β and IL-6 were expressed at 3h and 6h, and the level of HMGB1 significantly increased by 24h. Pretreatment with ethyl pyruvate ameliorated the pathological effects of Con A-induced autoimmune hepatitis and significantly decreased the levels of TNF-α, IL-2, IL-6 and IL-1β at 3h and 6h and the level of HMGB1 at 6h and 24h post injection. Ethyl pyruvate blocked the degradation of IκB α and IκB β and decreased the expression of NF-κB at 24h.Taken together, these results indicated that ethyl pyruvate protected against Con A-induced autoimmune hepatitis by decreasing both early (TNF-α, IL-2, IL-1β and IL-6) and late (HMGB1) cytokine expression in mice. The reduction of HMGB1 may correlate with the amelioration of NF-κB activity.http://europepmc.org/articles/PMC3912171?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Miao Shen Jie Lu Ping Cheng Chunlei Lin Weiqi Dai Fan Wang Chengfen Wang Yan Zhang Kan Chen Ling Xu Yinqun Zhou Chuanyong Guo |
spellingShingle |
Miao Shen Jie Lu Ping Cheng Chunlei Lin Weiqi Dai Fan Wang Chengfen Wang Yan Zhang Kan Chen Ling Xu Yinqun Zhou Chuanyong Guo Ethyl pyruvate pretreatment attenuates concanavalin a-induced autoimmune hepatitis in mice. PLoS ONE |
author_facet |
Miao Shen Jie Lu Ping Cheng Chunlei Lin Weiqi Dai Fan Wang Chengfen Wang Yan Zhang Kan Chen Ling Xu Yinqun Zhou Chuanyong Guo |
author_sort |
Miao Shen |
title |
Ethyl pyruvate pretreatment attenuates concanavalin a-induced autoimmune hepatitis in mice. |
title_short |
Ethyl pyruvate pretreatment attenuates concanavalin a-induced autoimmune hepatitis in mice. |
title_full |
Ethyl pyruvate pretreatment attenuates concanavalin a-induced autoimmune hepatitis in mice. |
title_fullStr |
Ethyl pyruvate pretreatment attenuates concanavalin a-induced autoimmune hepatitis in mice. |
title_full_unstemmed |
Ethyl pyruvate pretreatment attenuates concanavalin a-induced autoimmune hepatitis in mice. |
title_sort |
ethyl pyruvate pretreatment attenuates concanavalin a-induced autoimmune hepatitis in mice. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
Ethyl pyruvate (EP), a potent reactive oxygen species scavenger, has been reported to contribute to the inflammatory process. However, the protective effect of ethyl pyruvate on Concanavalin A (Con A)-induced autoimmune hepatitis have not been explored. Thus, the aims of this study are to investigate both the effects of ethyl pyruvate and its mechanism of protection on Con A-induced autoimmune hepatitis in mice.Acute autoimmune hepatitis was induced by Con A (20 mg/kg) in Balb/C mice; ethyl pyruvate (40 mg/kg and 80 mg/kg) was administrated 1h prior to the Con A injection. At 3h, 6h and 24h post Con A injection, histological grading, proinflammatory cytokine levels and nuclear factor kappa B (NF-κB) activity were determined.Following Con A challenge, cytokines TNF-α, IL-2, IL-1β and IL-6 were expressed at 3h and 6h, and the level of HMGB1 significantly increased by 24h. Pretreatment with ethyl pyruvate ameliorated the pathological effects of Con A-induced autoimmune hepatitis and significantly decreased the levels of TNF-α, IL-2, IL-6 and IL-1β at 3h and 6h and the level of HMGB1 at 6h and 24h post injection. Ethyl pyruvate blocked the degradation of IκB α and IκB β and decreased the expression of NF-κB at 24h.Taken together, these results indicated that ethyl pyruvate protected against Con A-induced autoimmune hepatitis by decreasing both early (TNF-α, IL-2, IL-1β and IL-6) and late (HMGB1) cytokine expression in mice. The reduction of HMGB1 may correlate with the amelioration of NF-κB activity. |
url |
http://europepmc.org/articles/PMC3912171?pdf=render |
work_keys_str_mv |
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