Isolation and characterization of circulating pro-vascular progenitor cell subsets from human whole blood samples
Summary: The examination of circulating pro-vascular progenitor cell frequency and function is integral in understanding aberrant blood vessel homeostasis in individuals with cardiometabolic disease. Here, we outline the characterization of progenitor cell subsets from peripheral blood using high al...
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2021-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166721000186 |
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doaj-ee0233a7125f4d2f9b9e43ed0d93e17b2021-03-22T12:53:09ZengElsevierSTAR Protocols2666-16672021-03-0121100311Isolation and characterization of circulating pro-vascular progenitor cell subsets from human whole blood samplesDaniella C. Terenzi0Ehab Bakbak1Justin Z. Trac2Mohammad Al-Omran3Adrian Quan4Hwee Teoh5Subodh Verma6David A. Hess7Division of Cardiac Surgery, Keenan Research Centre for Biomedical Science and Li Ka Shing Knowledge Institute of St. Michael’s Hospital, Toronto, ON, Canada; Institute of Medical Science, University of Toronto, Toronto, ON, Canada; Corresponding authorDivision of Cardiac Surgery, Keenan Research Centre for Biomedical Science and Li Ka Shing Knowledge Institute of St. Michael’s Hospital, Toronto, ON, Canada; Department of Pharmacology and Toxicology, University of Toronto, Toronto, ON, CanadaDepartment of Pharmacology and Toxicology, University of Toronto, Toronto, ON, CanadaInstitute of Medical Science, University of Toronto, Toronto, ON, Canada; Department of Pharmacology and Toxicology, University of Toronto, Toronto, ON, Canada; Division of Vascular Surgery, Keenan Research Centre for Biomedical Science and Li Ka Shing Knowledge Institute of St. Michael’s Hospital, Toronto, ON, Canada; Department of Surgery, University of Toronto, Toronto, ON, CanadaDivision of Cardiac Surgery, Keenan Research Centre for Biomedical Science and Li Ka Shing Knowledge Institute of St. Michael’s Hospital, Toronto, ON, CanadaInstitute of Medical Science, University of Toronto, Toronto, ON, Canada; Division of Endocrinology and Metabolism, Li Ka Shing Knowledge Institute of St. Michael’s Hospital, Toronto, ON, CanadaDivision of Cardiac Surgery, Keenan Research Centre for Biomedical Science and Li Ka Shing Knowledge Institute of St. Michael’s Hospital, Toronto, ON, Canada; Institute of Medical Science, University of Toronto, Toronto, ON, Canada; Department of Pharmacology and Toxicology, University of Toronto, Toronto, ON, Canada; Department of Surgery, University of Toronto, Toronto, ON, CanadaDepartment of Pharmacology and Toxicology, University of Toronto, Toronto, ON, Canada; Division of Vascular Surgery, Keenan Research Centre for Biomedical Science and Li Ka Shing Knowledge Institute of St. Michael’s Hospital, Toronto, ON, Canada; Molecular Medicine Research Laboratories, Robarts Research Institute, London, ON, Canada; Department of Physiology and Pharmacology, Western University, London, ON, Canada; Corresponding authorSummary: The examination of circulating pro-vascular progenitor cell frequency and function is integral in understanding aberrant blood vessel homeostasis in individuals with cardiometabolic disease. Here, we outline the characterization of progenitor cell subsets from peripheral blood using high aldehyde dehydrogenase (ALDH) activity, an intracellular detoxification enzyme previously associated with pro-vascular progenitor cell status. Using this protocol, cells can be examined by flow cytometry for ALDH activity and lineage restricted cell surface markers simultaneously.For complete details on the use and execution of this protocol, please refer to Terenzi et al. (2019) and Hess et al. (2019, 2020).http://www.sciencedirect.com/science/article/pii/S2666166721000186Cell biologyCell isolationFlow cytometry/mass cytometryStem cells |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Daniella C. Terenzi Ehab Bakbak Justin Z. Trac Mohammad Al-Omran Adrian Quan Hwee Teoh Subodh Verma David A. Hess |
| spellingShingle |
Daniella C. Terenzi Ehab Bakbak Justin Z. Trac Mohammad Al-Omran Adrian Quan Hwee Teoh Subodh Verma David A. Hess Isolation and characterization of circulating pro-vascular progenitor cell subsets from human whole blood samples STAR Protocols Cell biology Cell isolation Flow cytometry/mass cytometry Stem cells |
| author_facet |
Daniella C. Terenzi Ehab Bakbak Justin Z. Trac Mohammad Al-Omran Adrian Quan Hwee Teoh Subodh Verma David A. Hess |
| author_sort |
Daniella C. Terenzi |
| title |
Isolation and characterization of circulating pro-vascular progenitor cell subsets from human whole blood samples |
| title_short |
Isolation and characterization of circulating pro-vascular progenitor cell subsets from human whole blood samples |
| title_full |
Isolation and characterization of circulating pro-vascular progenitor cell subsets from human whole blood samples |
| title_fullStr |
Isolation and characterization of circulating pro-vascular progenitor cell subsets from human whole blood samples |
| title_full_unstemmed |
Isolation and characterization of circulating pro-vascular progenitor cell subsets from human whole blood samples |
| title_sort |
isolation and characterization of circulating pro-vascular progenitor cell subsets from human whole blood samples |
| publisher |
Elsevier |
| series |
STAR Protocols |
| issn |
2666-1667 |
| publishDate |
2021-03-01 |
| description |
Summary: The examination of circulating pro-vascular progenitor cell frequency and function is integral in understanding aberrant blood vessel homeostasis in individuals with cardiometabolic disease. Here, we outline the characterization of progenitor cell subsets from peripheral blood using high aldehyde dehydrogenase (ALDH) activity, an intracellular detoxification enzyme previously associated with pro-vascular progenitor cell status. Using this protocol, cells can be examined by flow cytometry for ALDH activity and lineage restricted cell surface markers simultaneously.For complete details on the use and execution of this protocol, please refer to Terenzi et al. (2019) and Hess et al. (2019, 2020). |
| topic |
Cell biology Cell isolation Flow cytometry/mass cytometry Stem cells |
| url |
http://www.sciencedirect.com/science/article/pii/S2666166721000186 |
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