Evaluation of cytotoxic and mutagenic action of novel surface active comb-like polyampholytes that are used for delivery of nucleic acids to target cells

• Main Authors: N. S. Finiuk, Y. Z. Filyak, N. M. Boiko, N. Y. Mitina, O. S. Zaichenko, R. S. Stoika
• Format: Article
• Language: English
• Published: Львівський національний університет імені Івана Франка 2013-09-01
• Series: Біологічні студії
• Subjects: polyampholytic carriers; cytotoxic impact; cultures of mammalian cells; ames test
• Online Access: http://publications.lnu.edu.ua/journals/index.php/biology/article/view/240
• ISSN: 1996-4536; 2311-0783

Rapid development of new nanomaterials and nanotechnologies is accompanied by significant achievements in various fields of medicine, industry and precise technology, as well as by undesirable effects on human health and environment. Our studies were focused on determination of toxicity in vitro and evaluation of the mutagenic activity of novel polyampholyte carriers of BG-2 type. It was shown that they did not exert cytotoxic and could be used in the in vitro experiments at a concentration less than 0.01%. The carriers were not capable of triggering gene mutations in the Ames test. At the absence of metabolic activation, all studied carriers lacked genotoxic effects. No mutagenic effect was observed for novel carriers after adding of the microsomal fraction of rat liver for both strains of Salmonella typhimurium, namely TA98 and TA100. This indicates a safety of novel polyampholyte carriers of BG-2 type as a tool for delivery of nucleic acids into target cells.