Phenotypic and Genotypic Determination of Antibiotic Resistances of Some Clinical Staphylococcus aureus Isolates

• Main Authors: Zerife ORHAN, Arzu KAYIŞ, İsmail AKYOL, Esra KAYA, Murat ARAL
• Format: Article
• Language: English
• Published: Bilimsel Tip Yayinevi 2017-09-01
• Series: Flora Infeksiyon Hastalıkları ve Klinik Mikrobiyoloji Dergisi
• Subjects: Antibiotic resistance genes; Polymerase chain reaction; Phoenix; Staphylococcus aureus
• Online Access: http://www.floradergisi.org/getFileContent.aspx?op=REDPDF&file_name=2017-22-03-107-109.pdf

Introduction: Staphylococcus aureus is a dangerous pathogen causing serious illnesses. The acquisitions of antibiotic resistance genes has largely led to the problem of finding effective treatments. This study was carried out to evaluate the relationship between the antibiotic susceptibility patterns of some antibiotics and antibiotic resistance genes in S. aureus isolates obtained from various clinical samples of patients. Materials and Methods: A total of 100 clinical S. aureus isolates were subjected to the antimicrobial susceptibility test. The genes associated with resistance to oxacilline (mecA), trimethoprim-sulfamethoxazole (TMP-SMX) (dfrA), erythromycin, clindamycin (ermA, ermB, ermC and msrA), quinupristin-dalfopristin (vatA, vatB, vatC) and ciprofloxacin (gyrA, gyrB) were investigated by PCR amplification. Results: Methicillin resistance ratio of one hundred S. aureus isolates was found 19%, and all of these isolates were carrying the mecA gene. Only 1% of the isolates were phenotypically resistant to TMP-SMX and carrying the dfrA gene. Erythromycin resistance ratio was found 26% phenotypically and 6% of the isolates were carrying both the ermA and the ermC genes, and 22% of them were carrying only the ermC gene. None of the isolates carried the ermB and msrA genes. None of the isolates was phenotypically resistant to quinupristin-dalfopristin and carried vatA, vatB, vatC resistance genes. All isolates were carrying the gyrA and gyrB resistance genes, but 6% of the isolates were phenotypically resistant to ciprofloxacin. Conclusion: According to the acquired results, phenotypic antibiotic susceptibility test results were partially similar to molecular observation. In pathogenic organisms, the use of rapid and reliable methods for determining antibiotic susceptibility is important in determining appropriate treatment outcomes. When biochemical methods and molecular approaches were combined, more accurate and reliable results were observed in a short time.