Development of an indirect enzyme-linked immunosorbent assay and lateral-flow test strips for pefloxacin and its analogues in chicken muscle samples

• Main Authors: Daniel Mukunzi, Steven Suryoprabowo, Shanshan Song, Liqiang Liu, Hua Kuang
• Format: Article
• Language: English
• Published: Taylor & Francis Group 2018-01-01
• Series: Food and Agricultural Immunology
• Subjects: pefloxacin; monoclonal antibody; indirect competitive enzyme-linked immunosorbent assay; lateral flow; strip
• Online Access: http://dx.doi.org/10.1080/09540105.2017.1406460

An anti-pefloxacin (PEF) monoclonal antibody (mAb), an indirect competitive enzyme-linked immunosorbent assay and lateral-flow test strip methods were developed to detect fluoroquinolone (FQ) residues in chicken muscle samples. Under optimised conditions, the anti-PEF mAb showed reasonable cross-reactivity with nine FQs with a limit of detection of 0.082 ng/mL assayed in 0.01 M phosphate buffered saline (PBS) solution. The intra- and inter-assay recoveries from spiked samples were within the range of 62.42–111.47% and 63.5–113.79%, respectively. The visual cut-off values of the lateral flow test strip in 0.01 M PBS and in food matrices were within the range of 2.5–50 ng/mL and 5–100 µg/kg, respectively. These results show that the anti-PEF mAb immunoassay and lateral flow test strip methods are suitable for simultaneous detection and routine monitoring of FQ residues in food.