MNX1 Promotes Malignant Progression of Cervical Cancer via Repressing the Transcription of p21cip1
Motor neuron and pancreas homeobox 1 (MNX1) is a development-related genes and has been found to be highly expressed in several cancers. However, its biological function in cervical cancer remains largely unexplored. QRT-PCR, western blot, and IHC showed that MNX1 was abnormally overexpressed in cer...
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doaj-ee1fa64f057e432e8b0eade8a03bf1172020-11-25T02:59:18ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2020-08-011010.3389/fonc.2020.01307551738MNX1 Promotes Malignant Progression of Cervical Cancer via Repressing the Transcription of p21cip1Biqing Zhu0Yaqin Wu1Jing Luo2Quanli Zhang3Jian Huang4Qian Li5Lin Xu6Lin Xu7Emei Lu8Binhui Ren9Binhui Ren10Department of Radiation Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical University Affiliated Cancer Hospital, Nanjing, ChinaDepartment of Radiation Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical University Affiliated Cancer Hospital, Nanjing, ChinaDepartment of Cardiothoracic Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, ChinaJiangsu Key Laboratory of Molecular and Translational Cancer Research, Nanjing, ChinaDepartment of Radiation Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical University Affiliated Cancer Hospital, Nanjing, ChinaDepartment of Radiation Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical University Affiliated Cancer Hospital, Nanjing, ChinaJiangsu Key Laboratory of Molecular and Translational Cancer Research, Nanjing, ChinaDepartment of Thoracic Surgery, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical University Affiliated Cancer Hospital, Nanjing, ChinaDepartment of Radiation Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical University Affiliated Cancer Hospital, Nanjing, ChinaJiangsu Key Laboratory of Molecular and Translational Cancer Research, Nanjing, ChinaDepartment of Thoracic Surgery, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research, Nanjing Medical University Affiliated Cancer Hospital, Nanjing, ChinaMotor neuron and pancreas homeobox 1 (MNX1) is a development-related genes and has been found to be highly expressed in several cancers. However, its biological function in cervical cancer remains largely unexplored. QRT-PCR, western blot, and IHC showed that MNX1 was abnormally overexpressed in cervical cancer tissues and cell lines. The high expression level of MNX1 correlated with poorer clinicopathologic characteristics in cervical cancer patients. Evaluated by RTCA (Real Time Cellular Analysis) proliferation assay, colony formation assay, EdU assay, transwell assay, and matrigel assay, we found that knockdown of MNX1 inhibited proliferation, migration and invasion of cervical cancer in vitro, while overexpression of MNX1 promoted malignant phenotype of cervical cancer. And subcutaneous xenograft model confirmed the malignant phenotype of MNX1 in vivo. Furthermore, flow cytometry, chromatin immunoprecipitation, and luciferase reporter assay indicated that MNX1 accelerated cell cycle transition by transcriptionally downregulating cyclin-dependent kinases p21cip1. In summary, our study revealed that MNX1 exerted an oncogenic role in cervical cancer via repressing the transcription of p21cip1 and thus accelerating cell cycle progression. Our results suggested that MNX1 was a potential diagnostic marker and therapeutic target for cervical cancer patients.https://www.frontiersin.org/article/10.3389/fonc.2020.01307/fullcervical cancerMNX1cell cycletranscriptionp21cip1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Biqing Zhu Yaqin Wu Jing Luo Quanli Zhang Jian Huang Qian Li Lin Xu Lin Xu Emei Lu Binhui Ren Binhui Ren |
spellingShingle |
Biqing Zhu Yaqin Wu Jing Luo Quanli Zhang Jian Huang Qian Li Lin Xu Lin Xu Emei Lu Binhui Ren Binhui Ren MNX1 Promotes Malignant Progression of Cervical Cancer via Repressing the Transcription of p21cip1 Frontiers in Oncology cervical cancer MNX1 cell cycle transcription p21cip1 |
author_facet |
Biqing Zhu Yaqin Wu Jing Luo Quanli Zhang Jian Huang Qian Li Lin Xu Lin Xu Emei Lu Binhui Ren Binhui Ren |
author_sort |
Biqing Zhu |
title |
MNX1 Promotes Malignant Progression of Cervical Cancer via Repressing the Transcription of p21cip1 |
title_short |
MNX1 Promotes Malignant Progression of Cervical Cancer via Repressing the Transcription of p21cip1 |
title_full |
MNX1 Promotes Malignant Progression of Cervical Cancer via Repressing the Transcription of p21cip1 |
title_fullStr |
MNX1 Promotes Malignant Progression of Cervical Cancer via Repressing the Transcription of p21cip1 |
title_full_unstemmed |
MNX1 Promotes Malignant Progression of Cervical Cancer via Repressing the Transcription of p21cip1 |
title_sort |
mnx1 promotes malignant progression of cervical cancer via repressing the transcription of p21cip1 |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Oncology |
issn |
2234-943X |
publishDate |
2020-08-01 |
description |
Motor neuron and pancreas homeobox 1 (MNX1) is a development-related genes and has been found to be highly expressed in several cancers. However, its biological function in cervical cancer remains largely unexplored. QRT-PCR, western blot, and IHC showed that MNX1 was abnormally overexpressed in cervical cancer tissues and cell lines. The high expression level of MNX1 correlated with poorer clinicopathologic characteristics in cervical cancer patients. Evaluated by RTCA (Real Time Cellular Analysis) proliferation assay, colony formation assay, EdU assay, transwell assay, and matrigel assay, we found that knockdown of MNX1 inhibited proliferation, migration and invasion of cervical cancer in vitro, while overexpression of MNX1 promoted malignant phenotype of cervical cancer. And subcutaneous xenograft model confirmed the malignant phenotype of MNX1 in vivo. Furthermore, flow cytometry, chromatin immunoprecipitation, and luciferase reporter assay indicated that MNX1 accelerated cell cycle transition by transcriptionally downregulating cyclin-dependent kinases p21cip1. In summary, our study revealed that MNX1 exerted an oncogenic role in cervical cancer via repressing the transcription of p21cip1 and thus accelerating cell cycle progression. Our results suggested that MNX1 was a potential diagnostic marker and therapeutic target for cervical cancer patients. |
topic |
cervical cancer MNX1 cell cycle transcription p21cip1 |
url |
https://www.frontiersin.org/article/10.3389/fonc.2020.01307/full |
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