Polydopamine-Supported Lipid Bilayers
We report the formation of lipid membranes supported by a soft polymeric cushion of polydopamine. First, 20 nm thick polydopamine films were formed on mica substrates. Atomic force microscopy imaging indicated that these films were also soft with a surface roughness of 2 nm under hydrated conditions...
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2012-12-01
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Online Access: | http://www.mdpi.com/1996-1944/5/12/2621 |
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doaj-ee2359219e8c4dbf8c7599329df615db2020-11-25T00:14:01ZengMDPI AGMaterials1996-19442012-12-015122621263610.3390/ma5122621Polydopamine-Supported Lipid BilayersSouryvanh NirasayAntonella BadiaGrégoire LeclairJerome P. ClaverieIsabelle MarcotteWe report the formation of lipid membranes supported by a soft polymeric cushion of polydopamine. First, 20 nm thick polydopamine films were formed on mica substrates. Atomic force microscopy imaging indicated that these films were also soft with a surface roughness of 2 nm under hydrated conditions. A zwitterionic phospholipid bilayer was then deposited on the polydopamine cushion by fusion of dimyristoylphosphatidylcholine (DMPC) and dioleoylphosphatidylcholine (DOPC) vesicles. Polydopamine films preserved the lateral mobility of the phospholipids as shown by fluorescence microscopy recovery after photobleaching (FRAP) experiments. Diffusion coefficients of ~5.9 and 7.2 µm2 s−1 were respectively determined for DMPC and DOPC at room temperature, values which are characteristic of lipids in a free standing bilayer system.http://www.mdpi.com/1996-1944/5/12/2621supported membranephospholipid bilayerpolymer cushionpolydopaminelipid diffusion |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Souryvanh Nirasay Antonella Badia Grégoire Leclair Jerome P. Claverie Isabelle Marcotte |
spellingShingle |
Souryvanh Nirasay Antonella Badia Grégoire Leclair Jerome P. Claverie Isabelle Marcotte Polydopamine-Supported Lipid Bilayers Materials supported membrane phospholipid bilayer polymer cushion polydopamine lipid diffusion |
author_facet |
Souryvanh Nirasay Antonella Badia Grégoire Leclair Jerome P. Claverie Isabelle Marcotte |
author_sort |
Souryvanh Nirasay |
title |
Polydopamine-Supported Lipid Bilayers |
title_short |
Polydopamine-Supported Lipid Bilayers |
title_full |
Polydopamine-Supported Lipid Bilayers |
title_fullStr |
Polydopamine-Supported Lipid Bilayers |
title_full_unstemmed |
Polydopamine-Supported Lipid Bilayers |
title_sort |
polydopamine-supported lipid bilayers |
publisher |
MDPI AG |
series |
Materials |
issn |
1996-1944 |
publishDate |
2012-12-01 |
description |
We report the formation of lipid membranes supported by a soft polymeric cushion of polydopamine. First, 20 nm thick polydopamine films were formed on mica substrates. Atomic force microscopy imaging indicated that these films were also soft with a surface roughness of 2 nm under hydrated conditions. A zwitterionic phospholipid bilayer was then deposited on the polydopamine cushion by fusion of dimyristoylphosphatidylcholine (DMPC) and dioleoylphosphatidylcholine (DOPC) vesicles. Polydopamine films preserved the lateral mobility of the phospholipids as shown by fluorescence microscopy recovery after photobleaching (FRAP) experiments. Diffusion coefficients of ~5.9 and 7.2 µm2 s−1 were respectively determined for DMPC and DOPC at room temperature, values which are characteristic of lipids in a free standing bilayer system. |
topic |
supported membrane phospholipid bilayer polymer cushion polydopamine lipid diffusion |
url |
http://www.mdpi.com/1996-1944/5/12/2621 |
work_keys_str_mv |
AT souryvanhnirasay polydopaminesupportedlipidbilayers AT antonellabadia polydopaminesupportedlipidbilayers AT gregoireleclair polydopaminesupportedlipidbilayers AT jeromepclaverie polydopaminesupportedlipidbilayers AT isabellemarcotte polydopaminesupportedlipidbilayers |
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