Validation of a Non-Specific Dye Real-Time PCR Assay for Porcine Adulteration in Meatball Using ND5 Primer

Validation of a Non-Specific Dye Real-Time PCR Assay for Porcine Adulteration in Meatball Using ND5 Primer

Porcine adulteration in meatball samples were analyzed using real-time polymerase chain reaction (RT-PCR), based on the ND5 primer obtained by previous study. This work consisted of three stages which were annealing temperature optimization, method validation, and application. DNA template was extra...

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Bibliographic Details
Main Authors: Tri Joko Raharjo, Ery Nourika Alfiraza, Esti Enjelina, Deni Pranowo
Format: Article
Language:English
Published: Universitas Gadjah Mada 2017-07-01
Series:Indonesian Journal of Chemistry
Subjects:
meatballs
porcine DNA
method validation
ND5 primer
real-time PCR
Online Access:https://jurnal.ugm.ac.id/ijc/article/view/22646

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