KLF5 expression in prostate cancer tissue and effect of KLF5 knockdown on prostate cancer cell growth

• Main Authors: Chong-Jun Shi, Wei-Zhong Yang, Yuan-Rong Kong, Wen-Guang Zhou
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2016-12-01
• Series: Journal of Hainan Medical University
• Subjects: Prostate cancer; Krüppel-like factor 5; Proliferation; Epithelial-mesenchymal transition
• Online Access: http://www.hnykdxxb.com/PDF/201624/1.pdf

Objective: To study the KLF5 expression in prostate cancer tissue and the effect of KLF5 knockdown on prostate cancer cell growth. Methods: Prostate cancer and benign prostatic hyperplasia tissue were collected to extract RNA and determine the mRNA levels of KLF5 as well as proliferation and epithelial-mesenchymal transition-related genes; DU145 cells were cultured and transfected with KLF5 siRNA and negative control siRNA, and then RNA was extracted to determine the mRNA levels of proliferation and epithelial-mesenchymal transitionrelated genes. Results: KLF5, SRSF1, Survivin, MACC1, c-Met, N-cadherin and Vimentin mRNA levels in prostate cancer tissue were significantly higher than those in benign prostatic hyperplasia tissue while TGF-β, E-cadherin and β-catenin mRNA levels were significantly lower than those in benign prostatic hyperplasia tissue; SRSF1, Survivin, MACC1, c-Met, TGF- β, N-cadherin and Vimentin mRNA levels in si-KLF5 group were significantly lower than those in si-NC group while E-cadherin and β-catenin mRNA levels were significantly higher than those in si-NC group. Conclusions: KLF5 expression levels are unusually high in prostate cancer tissue, and targeted knockdown of KLF5 expression can inhibit the prostate cancer cell proliferation and epithelial-mesenchymal transition.