Histomorphometric changes in the perirenal adipocytes of adrenalectomized rats treated with dexamethasone
INTRODUCTION: Prolonged steroid treatment administered to any patient can cause visceral obesity, which is associated with metabolic disease and Cushing's syndrome. Glucocorticoids have a profound negative effect on adipose tissue mass, giving rise to obesity, which in turn is regulated by the...
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doaj-ee37e2364f144bd49f531a02acb66d832020-11-24T21:25:56ZengFaculdade de Medicina / USPClinics1807-59321980-53222011-01-0166584985310.1590/S1807-59322011000500023Histomorphometric changes in the perirenal adipocytes of adrenalectomized rats treated with dexamethasoneFairus AhmadIma Nirwana SoelaimanElvy Suhana Mohd RamliTan Ming HooiFarihah H. SuhaimiINTRODUCTION: Prolonged steroid treatment administered to any patient can cause visceral obesity, which is associated with metabolic disease and Cushing's syndrome. Glucocorticoids have a profound negative effect on adipose tissue mass, giving rise to obesity, which in turn is regulated by the 11β-hydroxysteroid dehydrogenase type 1 enzyme. Adrenalectomized rats treated with dexamethasone exhibited an increase in visceral fat deposition but not in body weight. OBJECTIVES: The main aim of this study was to determine the effect of dexamethasone on the histomorphometric characteristics of perirenal adipocytes of adrenalectomized, dexamethasone-treated rats (ADR+Dexa) and the association of dexamethasone treatment with the expression and activity of 11 β-hydroxysteroid dehydrogenase type 1 (11 β-hydroxysteroid dehydrogenase type 1). METHODS: A total of 20 male Sprague Dawley rats were divided into 3 groups: a baseline control group (n = 6), a sham-operated group (n = 7) and an adrenalectomized group (n=7). The adrenalectomized group was given intramuscular dexamethasone (ADR+Dexa) 2 weeks post adrenalectomy, and the rats from the sham-operated group were administered intramuscular vehicle (olive oil). RESULTS: Treatment with 120 μg/kg intramuscular dexamethasone for 8 weeks resulted in a significant decrease in the diameter of the perirenal adipocytes (p<0.05) and a significant increase in the number of perirenal adipocytes (p<0.05). There was minimal weight gain but pronounced fat deposition in the dexamethasone-treated rats. These changes in the perirenal adipocytes were associated with high expression and dehydrogenase activity of 11β-hydroxysteroid dehydrogenase type 1. CONCLUSIONS: In conclusion, dexamethasone increased the deposition of perirenal fat by hyperplasia, which causes increases in the expression and dehydrogenase activity of 11 β-hydroxysteroid dehydrogenase type 1 in adrenalectomized rats.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322011000500023DexamethasoneAdrenalectomized ratPerirenal fatHyperplasia11 β-hydroxysteroid dehydrogenase type 1 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Fairus Ahmad Ima Nirwana Soelaiman Elvy Suhana Mohd Ramli Tan Ming Hooi Farihah H. Suhaimi |
spellingShingle |
Fairus Ahmad Ima Nirwana Soelaiman Elvy Suhana Mohd Ramli Tan Ming Hooi Farihah H. Suhaimi Histomorphometric changes in the perirenal adipocytes of adrenalectomized rats treated with dexamethasone Clinics Dexamethasone Adrenalectomized rat Perirenal fat Hyperplasia 11 β-hydroxysteroid dehydrogenase type 1 |
author_facet |
Fairus Ahmad Ima Nirwana Soelaiman Elvy Suhana Mohd Ramli Tan Ming Hooi Farihah H. Suhaimi |
author_sort |
Fairus Ahmad |
title |
Histomorphometric changes in the perirenal adipocytes of adrenalectomized rats treated with dexamethasone |
title_short |
Histomorphometric changes in the perirenal adipocytes of adrenalectomized rats treated with dexamethasone |
title_full |
Histomorphometric changes in the perirenal adipocytes of adrenalectomized rats treated with dexamethasone |
title_fullStr |
Histomorphometric changes in the perirenal adipocytes of adrenalectomized rats treated with dexamethasone |
title_full_unstemmed |
Histomorphometric changes in the perirenal adipocytes of adrenalectomized rats treated with dexamethasone |
title_sort |
histomorphometric changes in the perirenal adipocytes of adrenalectomized rats treated with dexamethasone |
publisher |
Faculdade de Medicina / USP |
series |
Clinics |
issn |
1807-5932 1980-5322 |
publishDate |
2011-01-01 |
description |
INTRODUCTION: Prolonged steroid treatment administered to any patient can cause visceral obesity, which is associated with metabolic disease and Cushing's syndrome. Glucocorticoids have a profound negative effect on adipose tissue mass, giving rise to obesity, which in turn is regulated by the 11β-hydroxysteroid dehydrogenase type 1 enzyme. Adrenalectomized rats treated with dexamethasone exhibited an increase in visceral fat deposition but not in body weight. OBJECTIVES: The main aim of this study was to determine the effect of dexamethasone on the histomorphometric characteristics of perirenal adipocytes of adrenalectomized, dexamethasone-treated rats (ADR+Dexa) and the association of dexamethasone treatment with the expression and activity of 11 β-hydroxysteroid dehydrogenase type 1 (11 β-hydroxysteroid dehydrogenase type 1). METHODS: A total of 20 male Sprague Dawley rats were divided into 3 groups: a baseline control group (n = 6), a sham-operated group (n = 7) and an adrenalectomized group (n=7). The adrenalectomized group was given intramuscular dexamethasone (ADR+Dexa) 2 weeks post adrenalectomy, and the rats from the sham-operated group were administered intramuscular vehicle (olive oil). RESULTS: Treatment with 120 μg/kg intramuscular dexamethasone for 8 weeks resulted in a significant decrease in the diameter of the perirenal adipocytes (p<0.05) and a significant increase in the number of perirenal adipocytes (p<0.05). There was minimal weight gain but pronounced fat deposition in the dexamethasone-treated rats. These changes in the perirenal adipocytes were associated with high expression and dehydrogenase activity of 11β-hydroxysteroid dehydrogenase type 1. CONCLUSIONS: In conclusion, dexamethasone increased the deposition of perirenal fat by hyperplasia, which causes increases in the expression and dehydrogenase activity of 11 β-hydroxysteroid dehydrogenase type 1 in adrenalectomized rats. |
topic |
Dexamethasone Adrenalectomized rat Perirenal fat Hyperplasia 11 β-hydroxysteroid dehydrogenase type 1 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322011000500023 |
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