Data describing the effects of potassium channels modulators on outward currents measured in human lymphoma cell lines
In the present work, applying the whole-cell patch-clamp technique in voltage clamp mode, we have investigated the effects of different drugs, such as riluzole, Psora-4 and Tram-34, on the potassium currents in four human lymphoma cell lines. We focused on outward currents mediated by two potassium...
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doaj-ee43703e668349198dfd8f323b2ea0852020-12-25T05:10:39ZengElsevierData in Brief2352-34092021-02-0134106668Data describing the effects of potassium channels modulators on outward currents measured in human lymphoma cell linesAlberto Montalbano0Cesare Sala1Chiara Abrardo2Nicoletta Murciano3Farhad Jahanfar4Massimo D'Amico5Francesco Bertoni6Andrea Becchetti7Annarosa Arcangeli8Department of Experimental and Clinical Medicine, University of Florence, I‑50134 Florence, Italy; Corresponding author.Department of Experimental and Clinical Medicine, University of Florence, I‑50134 Florence, ItalyDepartment of Experimental and Clinical Medicine, University of Florence, I‑50134 Florence, ItalyDepartment of Experimental and Clinical Medicine, University of Florence, I‑50134 Florence, ItalyDIVAL Toscana Srl, Via Madonna del Piano 6, Sesto Fiorentino, I-50119 Firenze, Italy; Department of Medical Biotechnologies, University of Siena, I-53100 Siena, ItalyDIVAL Toscana Srl, Via Madonna del Piano 6, Sesto Fiorentino, I-50119 Firenze, ItalyInstitute of Oncology Research, Faculty of Biomedical Sciences, USI, via Vela 6, CH-6500 Bellinzona, Switzerland; Oncology Institute of Southern Switzerland (IOSI), Ospedale San Giovanni, CH-6500 Bellinzona, SwitzerlandDepartment of Biotechnology and Biosciences, University of Milano-Bicocca, Piazza della Scienza 2, I-20126 Milano, ItalyDepartment of Experimental and Clinical Medicine, University of Florence, I‑50134 Florence, ItalyIn the present work, applying the whole-cell patch-clamp technique in voltage clamp mode, we have investigated the effects of different drugs, such as riluzole, Psora-4 and Tram-34, on the potassium currents in four human lymphoma cell lines. We focused on outward currents mediated by two potassium channels (Kv1.3 and KCa3.1), which are known to play a key physiological role in lymphoid cells. The currents were evoked by voltage ramps ranging from -120 mV to +40 mV and the conductance of the two potassium channels was measured between +20 mV and +40 mV, both in the absence and in the presence of the specific blockers Psora-4 (Kv1.3; 1 µM) and Tram-34 (KCa3.1; 1 µM). The effect of the latter was tested after KCa3.1 channels were activated by riluzole 10 µM. Taken together, these data could be useful as an indication of the functional characteristics of the potassium channels in human lymphomas and represent a starting point for the study of potassium conductance in cellular models of these tumors.http://www.sciencedirect.com/science/article/pii/S235234092031547XHuman lymphomasPotassium channelsKv1.3KCa3.1Patch-clamp |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Alberto Montalbano Cesare Sala Chiara Abrardo Nicoletta Murciano Farhad Jahanfar Massimo D'Amico Francesco Bertoni Andrea Becchetti Annarosa Arcangeli |
spellingShingle |
Alberto Montalbano Cesare Sala Chiara Abrardo Nicoletta Murciano Farhad Jahanfar Massimo D'Amico Francesco Bertoni Andrea Becchetti Annarosa Arcangeli Data describing the effects of potassium channels modulators on outward currents measured in human lymphoma cell lines Data in Brief Human lymphomas Potassium channels Kv1.3 KCa3.1 Patch-clamp |
author_facet |
Alberto Montalbano Cesare Sala Chiara Abrardo Nicoletta Murciano Farhad Jahanfar Massimo D'Amico Francesco Bertoni Andrea Becchetti Annarosa Arcangeli |
author_sort |
Alberto Montalbano |
title |
Data describing the effects of potassium channels modulators on outward currents measured in human lymphoma cell lines |
title_short |
Data describing the effects of potassium channels modulators on outward currents measured in human lymphoma cell lines |
title_full |
Data describing the effects of potassium channels modulators on outward currents measured in human lymphoma cell lines |
title_fullStr |
Data describing the effects of potassium channels modulators on outward currents measured in human lymphoma cell lines |
title_full_unstemmed |
Data describing the effects of potassium channels modulators on outward currents measured in human lymphoma cell lines |
title_sort |
data describing the effects of potassium channels modulators on outward currents measured in human lymphoma cell lines |
publisher |
Elsevier |
series |
Data in Brief |
issn |
2352-3409 |
publishDate |
2021-02-01 |
description |
In the present work, applying the whole-cell patch-clamp technique in voltage clamp mode, we have investigated the effects of different drugs, such as riluzole, Psora-4 and Tram-34, on the potassium currents in four human lymphoma cell lines. We focused on outward currents mediated by two potassium channels (Kv1.3 and KCa3.1), which are known to play a key physiological role in lymphoid cells. The currents were evoked by voltage ramps ranging from -120 mV to +40 mV and the conductance of the two potassium channels was measured between +20 mV and +40 mV, both in the absence and in the presence of the specific blockers Psora-4 (Kv1.3; 1 µM) and Tram-34 (KCa3.1; 1 µM). The effect of the latter was tested after KCa3.1 channels were activated by riluzole 10 µM. Taken together, these data could be useful as an indication of the functional characteristics of the potassium channels in human lymphomas and represent a starting point for the study of potassium conductance in cellular models of these tumors. |
topic |
Human lymphomas Potassium channels Kv1.3 KCa3.1 Patch-clamp |
url |
http://www.sciencedirect.com/science/article/pii/S235234092031547X |
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