Early Low-Fluence Red Light or Darkness Modulates the Shoot Regeneration Capacity of Excised <i>Arabidopsis</i> Roots
In plants, light is an important environmental signal that induces meristem development and interacts with endogenous signals, including hormones. We found that treatment with 24 h of low-fluence red light (24 h R) or 24 h of darkness (24 h D) following root excision greatly increased the frequency...
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doaj-ee4d241e741c47ffaaad1d15820a362d2020-11-25T03:55:40ZengMDPI AGPlants2223-77472020-10-0191378137810.3390/plants9101378Early Low-Fluence Red Light or Darkness Modulates the Shoot Regeneration Capacity of Excised <i>Arabidopsis</i> RootsXi Wei0Yanpeng Ding1Ye Wang2Fuguang Li3Xiaoyang Ge4Henan Normal University Research Base of State Key Laboratory of Cotton Biology, Xinxiang 453000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, ChinaHenan Normal University Research Base of State Key Laboratory of Cotton Biology, Xinxiang 453000, ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research of CAAS, Anyang 455000, ChinaIn plants, light is an important environmental signal that induces meristem development and interacts with endogenous signals, including hormones. We found that treatment with 24 h of low-fluence red light (24 h R) or 24 h of darkness (24 h D) following root excision greatly increased the frequency of shoot generation, while continuous low-fluence red light in callus and shoot induction stages blocked the explants’ ability to generate shoots. Shoot generation ability was closely associated with <i>WUS</i> expression and distribution pattern. 1-N-naphthylphtalamic acid (NPA) disrupted the dynamic distribution of the <i>WUS</i> signal induced by early 24 h R treatment, and NPA plus 24 R treatment increased the average shoot number compared with early 24 h R alone. Transcriptome analysis revealed that differentially expressed genes involved in meristem development and hormone signal pathways were significantly enriched during 24 R or 24 D induced shoot regeneration, where early 24 h R or 24 h D treatment upregulated expression of <i>WOX5</i>, <i>LBD</i>16, <i>LBD</i>18 and <i>PLT</i>3 to promote callus initiation and formation of root primordia, and also activated <i>WUS</i>, <i>STM</i>, <i>CUC</i>1 and <i>CUC</i>2 expression, leading to initiation of the shoot apical meristem (SAM). This finding demonstrates that early exposure of explants to transient low-fluence red light or darkness modulates the expression of marker genes related with callus development and shoot regeneration, and dynamic distribution of <i>WUS</i>, leading to an increased ability to generate shoots.https://www.mdpi.com/2223-7747/9/10/1378low-fluencered lightshoot regeneration<i>WUS</i>NPA |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Xi Wei Yanpeng Ding Ye Wang Fuguang Li Xiaoyang Ge |
spellingShingle |
Xi Wei Yanpeng Ding Ye Wang Fuguang Li Xiaoyang Ge Early Low-Fluence Red Light or Darkness Modulates the Shoot Regeneration Capacity of Excised <i>Arabidopsis</i> Roots Plants low-fluence red light shoot regeneration <i>WUS</i> NPA |
author_facet |
Xi Wei Yanpeng Ding Ye Wang Fuguang Li Xiaoyang Ge |
author_sort |
Xi Wei |
title |
Early Low-Fluence Red Light or Darkness Modulates the Shoot Regeneration Capacity of Excised <i>Arabidopsis</i> Roots |
title_short |
Early Low-Fluence Red Light or Darkness Modulates the Shoot Regeneration Capacity of Excised <i>Arabidopsis</i> Roots |
title_full |
Early Low-Fluence Red Light or Darkness Modulates the Shoot Regeneration Capacity of Excised <i>Arabidopsis</i> Roots |
title_fullStr |
Early Low-Fluence Red Light or Darkness Modulates the Shoot Regeneration Capacity of Excised <i>Arabidopsis</i> Roots |
title_full_unstemmed |
Early Low-Fluence Red Light or Darkness Modulates the Shoot Regeneration Capacity of Excised <i>Arabidopsis</i> Roots |
title_sort |
early low-fluence red light or darkness modulates the shoot regeneration capacity of excised <i>arabidopsis</i> roots |
publisher |
MDPI AG |
series |
Plants |
issn |
2223-7747 |
publishDate |
2020-10-01 |
description |
In plants, light is an important environmental signal that induces meristem development and interacts with endogenous signals, including hormones. We found that treatment with 24 h of low-fluence red light (24 h R) or 24 h of darkness (24 h D) following root excision greatly increased the frequency of shoot generation, while continuous low-fluence red light in callus and shoot induction stages blocked the explants’ ability to generate shoots. Shoot generation ability was closely associated with <i>WUS</i> expression and distribution pattern. 1-N-naphthylphtalamic acid (NPA) disrupted the dynamic distribution of the <i>WUS</i> signal induced by early 24 h R treatment, and NPA plus 24 R treatment increased the average shoot number compared with early 24 h R alone. Transcriptome analysis revealed that differentially expressed genes involved in meristem development and hormone signal pathways were significantly enriched during 24 R or 24 D induced shoot regeneration, where early 24 h R or 24 h D treatment upregulated expression of <i>WOX5</i>, <i>LBD</i>16, <i>LBD</i>18 and <i>PLT</i>3 to promote callus initiation and formation of root primordia, and also activated <i>WUS</i>, <i>STM</i>, <i>CUC</i>1 and <i>CUC</i>2 expression, leading to initiation of the shoot apical meristem (SAM). This finding demonstrates that early exposure of explants to transient low-fluence red light or darkness modulates the expression of marker genes related with callus development and shoot regeneration, and dynamic distribution of <i>WUS</i>, leading to an increased ability to generate shoots. |
topic |
low-fluence red light shoot regeneration <i>WUS</i> NPA |
url |
https://www.mdpi.com/2223-7747/9/10/1378 |
work_keys_str_mv |
AT xiwei earlylowfluenceredlightordarknessmodulatestheshootregenerationcapacityofexcisediarabidopsisiroots AT yanpengding earlylowfluenceredlightordarknessmodulatestheshootregenerationcapacityofexcisediarabidopsisiroots AT yewang earlylowfluenceredlightordarknessmodulatestheshootregenerationcapacityofexcisediarabidopsisiroots AT fuguangli earlylowfluenceredlightordarknessmodulatestheshootregenerationcapacityofexcisediarabidopsisiroots AT xiaoyangge earlylowfluenceredlightordarknessmodulatestheshootregenerationcapacityofexcisediarabidopsisiroots |
_version_ |
1724468830601740288 |