In Vitro Screening of Falcataria moluccana (Miq.) with Gall Rust (Uromycladium tepperianum (Sacc.) Filtrate as Media Selection
In vitro screening of Falcataria moluccana (Miq.) was conducted by tissue culture method. Seeds from<br />two different site of community forest, 400 m (S1) and 800 m (S2) above sea level, were used as material.<br />Double concentration of MS (Murashige & Skoog, 1962) with 40 mg...
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doaj-ee5027170d94402cb5ac506fc8cf3c272020-11-24T21:54:20ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412016-02-011927471In Vitro Screening of Falcataria moluccana (Miq.) with Gall Rust (Uromycladium tepperianum (Sacc.) Filtrate as Media SelectionAsri Insiana Putri0Mohammad Na'iem1Sapto Indrioko2Sri Rahayu3Ari Indrianto4Center of Forest Biotechnology and Tree Improvement, FORDA, Ministry of Forestry, Indonesia.Faculty of Forestry, Gadjah Mada University, Yogyakarta, IndonesiaFaculty of Forestry, Gadjah Mada University, Yogyakarta, IndonesiaFaculty of Forestry, Gadjah Mada University, Yogyakarta, IndonesiaFaculty of Biology, Gadjah Mada University, Yogyakarta, IndonesiaIn vitro screening of Falcataria moluccana (Miq.) was conducted by tissue culture method. Seeds from<br />two different site of community forest, 400 m (S1) and 800 m (S2) above sea level, were used as material.<br />Double concentration of MS (Murashige & Skoog, 1962) with 40 mg/l gall rust (Uromycladium tepperianum<br />(Sacc.) fi ltrate were used for media selection. The results of this research showed that 66 % axenic plantlets in<br />vitro from S1 and 27 % from S2 were still survived after 3 months incubation without subculture. The mean<br />of fresh weight (2. 21 ± 0. 26 g) and dry weight (1. 97 ± 0. 12 g) from S1 plantlets lower than the mean of fresh<br />weight (2. 87 ± 0,18 g) and dry weight (2. 16 ± 0. 14 g) from S2 plantlets. Qualitative of terpenes, saponins and<br />quantitative of total phenolics were analyzed from those gall rust extract, as source of fi ltrate media, attacked<br />and un-attacked of F. moluccana. They all qualitatively have capability to produce terpenoid and saponin. It<br />is notice that U. tepperianum, un-cultured pathogen, contain of those compound that may play a role as codeterminants<br />of pathogenecity. While the highest total phenolic compound were contained in gall rust extract<br />(2. 35 %), followed by attacked F. moluccana branches (1. 18 %) and un-attacked F. moluccana branches (0. 44<br />%). This indicated that phenolic compound in gall rust has higher activity as a response of F. moluccana to U.<br />tepperianum pathogen pressures and result of this study suggest the great value of gall rust fi ltrate for use as<br />media selection in vitro.http://journal.ugm.ac.id/ijbiotech/article/view/9301F. moluccana, U. tepperianum, gall rust, in vitro selection |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Asri Insiana Putri Mohammad Na'iem Sapto Indrioko Sri Rahayu Ari Indrianto |
spellingShingle |
Asri Insiana Putri Mohammad Na'iem Sapto Indrioko Sri Rahayu Ari Indrianto In Vitro Screening of Falcataria moluccana (Miq.) with Gall Rust (Uromycladium tepperianum (Sacc.) Filtrate as Media Selection Indonesian Journal of Biotechnology F. moluccana, U. tepperianum, gall rust, in vitro selection |
author_facet |
Asri Insiana Putri Mohammad Na'iem Sapto Indrioko Sri Rahayu Ari Indrianto |
author_sort |
Asri Insiana Putri |
title |
In Vitro Screening of Falcataria moluccana (Miq.) with Gall Rust (Uromycladium tepperianum (Sacc.) Filtrate as Media Selection |
title_short |
In Vitro Screening of Falcataria moluccana (Miq.) with Gall Rust (Uromycladium tepperianum (Sacc.) Filtrate as Media Selection |
title_full |
In Vitro Screening of Falcataria moluccana (Miq.) with Gall Rust (Uromycladium tepperianum (Sacc.) Filtrate as Media Selection |
title_fullStr |
In Vitro Screening of Falcataria moluccana (Miq.) with Gall Rust (Uromycladium tepperianum (Sacc.) Filtrate as Media Selection |
title_full_unstemmed |
In Vitro Screening of Falcataria moluccana (Miq.) with Gall Rust (Uromycladium tepperianum (Sacc.) Filtrate as Media Selection |
title_sort |
in vitro screening of falcataria moluccana (miq.) with gall rust (uromycladium tepperianum (sacc.) filtrate as media selection |
publisher |
Universitas Gadjah Mada, Yogyakarta |
series |
Indonesian Journal of Biotechnology |
issn |
0853-8654 2089-2241 |
publishDate |
2016-02-01 |
description |
In vitro screening of Falcataria moluccana (Miq.) was conducted by tissue culture method. Seeds from<br />two different site of community forest, 400 m (S1) and 800 m (S2) above sea level, were used as material.<br />Double concentration of MS (Murashige & Skoog, 1962) with 40 mg/l gall rust (Uromycladium tepperianum<br />(Sacc.) fi ltrate were used for media selection. The results of this research showed that 66 % axenic plantlets in<br />vitro from S1 and 27 % from S2 were still survived after 3 months incubation without subculture. The mean<br />of fresh weight (2. 21 ± 0. 26 g) and dry weight (1. 97 ± 0. 12 g) from S1 plantlets lower than the mean of fresh<br />weight (2. 87 ± 0,18 g) and dry weight (2. 16 ± 0. 14 g) from S2 plantlets. Qualitative of terpenes, saponins and<br />quantitative of total phenolics were analyzed from those gall rust extract, as source of fi ltrate media, attacked<br />and un-attacked of F. moluccana. They all qualitatively have capability to produce terpenoid and saponin. It<br />is notice that U. tepperianum, un-cultured pathogen, contain of those compound that may play a role as codeterminants<br />of pathogenecity. While the highest total phenolic compound were contained in gall rust extract<br />(2. 35 %), followed by attacked F. moluccana branches (1. 18 %) and un-attacked F. moluccana branches (0. 44<br />%). This indicated that phenolic compound in gall rust has higher activity as a response of F. moluccana to U.<br />tepperianum pathogen pressures and result of this study suggest the great value of gall rust fi ltrate for use as<br />media selection in vitro. |
topic |
F. moluccana, U. tepperianum, gall rust, in vitro selection |
url |
http://journal.ugm.ac.id/ijbiotech/article/view/9301 |
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