Transcriptomic analysis of acclimation to temperature and light stress in Saccharina latissima (Phaeophyceae).
Kelps, brown algae of the order Laminariales, dominate rocky shores and form huge kelp beds which provide habitat and nurseries for various marine organisms. Whereas the basic physiological and ecophysiological characteristics of kelps are well studied, the molecular processes underlying acclimation...
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2012-01-01
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doaj-ee525a7ac8404a098cf9ed651593a65a2020-11-24T20:40:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-0178e4434210.1371/journal.pone.0044342Transcriptomic analysis of acclimation to temperature and light stress in Saccharina latissima (Phaeophyceae).Sandra HeinrichKlaus ValentinStephan FrickenhausUwe JohnChristian WienckeKelps, brown algae of the order Laminariales, dominate rocky shores and form huge kelp beds which provide habitat and nurseries for various marine organisms. Whereas the basic physiological and ecophysiological characteristics of kelps are well studied, the molecular processes underlying acclimation to different light and temperature conditions are still poorly understood. Therefore we investigated the molecular mechanisms underlying the physiological acclimation to light and temperature stress. Sporophytes of S. latissima were exposed to combinations of light intensities and temperatures, and microarray hybridizations were performed to determine changes in gene expression patterns. This first large-scale transcriptomic study of a kelp species shows that S. latissima responds to temperature and light stress with a multitude of transcriptional changes: up to 32% of genes showed an altered expression after the exposure experiments. High temperature had stronger effects on gene expression in S. latissima than low temperature, reflected by the higher number of temperature-responsive genes. We gained insights into underlying molecular processes of acclimation, which includes adjustment of the primary metabolism as well as induction of several ROS scavengers and a sophisticated regulation of Hsps. We show that S. latissima, as a cold adapted species, must make stronger efforts for acclimating to high than to low temperatures. The strongest response was caused by the combination of high temperatures with high light intensities, which proved most harmful for the alga.http://europepmc.org/articles/PMC3429442?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sandra Heinrich Klaus Valentin Stephan Frickenhaus Uwe John Christian Wiencke |
spellingShingle |
Sandra Heinrich Klaus Valentin Stephan Frickenhaus Uwe John Christian Wiencke Transcriptomic analysis of acclimation to temperature and light stress in Saccharina latissima (Phaeophyceae). PLoS ONE |
author_facet |
Sandra Heinrich Klaus Valentin Stephan Frickenhaus Uwe John Christian Wiencke |
author_sort |
Sandra Heinrich |
title |
Transcriptomic analysis of acclimation to temperature and light stress in Saccharina latissima (Phaeophyceae). |
title_short |
Transcriptomic analysis of acclimation to temperature and light stress in Saccharina latissima (Phaeophyceae). |
title_full |
Transcriptomic analysis of acclimation to temperature and light stress in Saccharina latissima (Phaeophyceae). |
title_fullStr |
Transcriptomic analysis of acclimation to temperature and light stress in Saccharina latissima (Phaeophyceae). |
title_full_unstemmed |
Transcriptomic analysis of acclimation to temperature and light stress in Saccharina latissima (Phaeophyceae). |
title_sort |
transcriptomic analysis of acclimation to temperature and light stress in saccharina latissima (phaeophyceae). |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2012-01-01 |
description |
Kelps, brown algae of the order Laminariales, dominate rocky shores and form huge kelp beds which provide habitat and nurseries for various marine organisms. Whereas the basic physiological and ecophysiological characteristics of kelps are well studied, the molecular processes underlying acclimation to different light and temperature conditions are still poorly understood. Therefore we investigated the molecular mechanisms underlying the physiological acclimation to light and temperature stress. Sporophytes of S. latissima were exposed to combinations of light intensities and temperatures, and microarray hybridizations were performed to determine changes in gene expression patterns. This first large-scale transcriptomic study of a kelp species shows that S. latissima responds to temperature and light stress with a multitude of transcriptional changes: up to 32% of genes showed an altered expression after the exposure experiments. High temperature had stronger effects on gene expression in S. latissima than low temperature, reflected by the higher number of temperature-responsive genes. We gained insights into underlying molecular processes of acclimation, which includes adjustment of the primary metabolism as well as induction of several ROS scavengers and a sophisticated regulation of Hsps. We show that S. latissima, as a cold adapted species, must make stronger efforts for acclimating to high than to low temperatures. The strongest response was caused by the combination of high temperatures with high light intensities, which proved most harmful for the alga. |
url |
http://europepmc.org/articles/PMC3429442?pdf=render |
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