A comparative Analysis of Antioxidant capacity of aqueous and methanolic leaf extracts of Scoparia dulcis and Schleichera oleosa
In this study a comparative analysis of total anti-oxidant capacity of aqueous and methanolic leaf extracts of Scoparia dulcis and Schleichera oleosa was done by estimation of DPPH radical, hydroxyl radical, superoxide anion radical and nitric oxide radical scavenging activity. The methanolic extr...
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Romanian Association of Balneology, Editura Balneara
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doaj-ee54c0ad804b48f3a60b84626bec06bf2020-11-24T23:10:35ZengRomanian Association of Balneology, Editura BalnearaBalneo Research Journal2069-75972069-76192018-09-019322122710.12680/balneo.2018.185A comparative Analysis of Antioxidant capacity of aqueous and methanolic leaf extracts of Scoparia dulcis and Schleichera oleosaSophy Jose0Manoranjan Prasad Sinha1*Department of Zoology, Ranchi University, Ranchi-834008, Jharkhand, India.*Department of Zoology, Ranchi University, Ranchi-834008, Jharkhand, India.In this study a comparative analysis of total anti-oxidant capacity of aqueous and methanolic leaf extracts of Scoparia dulcis and Schleichera oleosa was done by estimation of DPPH radical, hydroxyl radical, superoxide anion radical and nitric oxide radical scavenging activity. The methanolic extract (M.E) of S.oleosa showed a maximum concentration of total phenolics and flavanoids up to 20% and 168.89 % and aqueous extract (A.E) showed a maximum concentration of total phenolics and flavanoids up to 4.90% and 9.33% respectively. The M.E of S.dulcis showed a maximum concentration of total phenolics and flavanoids up to 6.21% and 9.24 % and A.E 46.22% and 14.67% respectively. Free radical scavenging activity was observed in both the M.E and A.E. For S.dulcis the % of free radical activity in M.E and A.E in 100µg concentration was 2.98% and 15.53% respectively. M.E and A.E of S.oleosa showed 54.94% and 5.93 % free radical scavenging activity correspondingly. Hydroxyl scavenging activity for 100µg of S.dulcis extracts were 17.92% in M.E and 11.39% in A.E where as for S.oleosa extracts it was 35.83% and 8.19% in M.E and A.E respectively. S.dulcis showed only 4.03% and 3.23% scavenging of superoxide anion radical and S.oleosa showed superoxide anion scavenging up to 22.58% in M.E and 3.23% in A.E. Nitric oxide scavenging activity was 5.71% in M.E of S.dulcis and 5.24% for A.E where as S. oleosa showed 33.81% nitric oxide scavenging activity in M.E and 31.43 % in A.E. The presence of phenol, flavonoid and total antioxidant in both the extract justifies the antioxidant potential of the plants which brings about their free radicals scavenging potential. Thus we conclude that the antioxidant activities may be due to the cumulative effect of the phytochemicals present in the plant which genuinely designate them as free radical scavengers.http://bioclima.ro/Balneo185.pdfExtractsTotal phenolic compoundsTotal flavonoidsNO radicalDPPH radicalhydroxyl radicalsuperoxide anion radical |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sophy Jose Manoranjan Prasad Sinha |
spellingShingle |
Sophy Jose Manoranjan Prasad Sinha A comparative Analysis of Antioxidant capacity of aqueous and methanolic leaf extracts of Scoparia dulcis and Schleichera oleosa Balneo Research Journal Extracts Total phenolic compounds Total flavonoids NO radical DPPH radical hydroxyl radical superoxide anion radical |
author_facet |
Sophy Jose Manoranjan Prasad Sinha |
author_sort |
Sophy Jose |
title |
A comparative Analysis of Antioxidant capacity of aqueous and methanolic leaf extracts of Scoparia dulcis and Schleichera oleosa |
title_short |
A comparative Analysis of Antioxidant capacity of aqueous and methanolic leaf extracts of Scoparia dulcis and Schleichera oleosa |
title_full |
A comparative Analysis of Antioxidant capacity of aqueous and methanolic leaf extracts of Scoparia dulcis and Schleichera oleosa |
title_fullStr |
A comparative Analysis of Antioxidant capacity of aqueous and methanolic leaf extracts of Scoparia dulcis and Schleichera oleosa |
title_full_unstemmed |
A comparative Analysis of Antioxidant capacity of aqueous and methanolic leaf extracts of Scoparia dulcis and Schleichera oleosa |
title_sort |
comparative analysis of antioxidant capacity of aqueous and methanolic leaf extracts of scoparia dulcis and schleichera oleosa |
publisher |
Romanian Association of Balneology, Editura Balneara |
series |
Balneo Research Journal |
issn |
2069-7597 2069-7619 |
publishDate |
2018-09-01 |
description |
In this study a comparative analysis of total anti-oxidant capacity of aqueous and methanolic leaf extracts of Scoparia dulcis and
Schleichera oleosa was done by estimation of DPPH radical, hydroxyl radical, superoxide anion radical and nitric oxide radical
scavenging activity. The methanolic extract (M.E) of S.oleosa showed a maximum concentration of total phenolics and flavanoids
up to 20% and 168.89 % and aqueous extract (A.E) showed a maximum concentration of total phenolics and flavanoids up to 4.90%
and 9.33% respectively. The M.E of S.dulcis showed a maximum concentration of total phenolics and flavanoids up to 6.21% and
9.24 % and A.E 46.22% and 14.67% respectively. Free radical scavenging activity was observed in both the M.E and A.E. For
S.dulcis the % of free radical activity in M.E and A.E in 100µg concentration was 2.98% and 15.53% respectively. M.E and A.E of
S.oleosa showed 54.94% and 5.93 % free radical scavenging activity correspondingly. Hydroxyl scavenging activity for 100µg of
S.dulcis extracts were 17.92% in M.E and 11.39% in A.E where as for S.oleosa extracts it was 35.83% and 8.19% in M.E and A.E
respectively. S.dulcis showed only 4.03% and 3.23% scavenging of superoxide anion radical and S.oleosa showed superoxide anion
scavenging up to 22.58% in M.E and 3.23% in A.E. Nitric oxide scavenging activity was 5.71% in M.E of S.dulcis and 5.24% for
A.E where as S. oleosa showed 33.81% nitric oxide scavenging activity in M.E and 31.43 % in A.E. The presence of phenol,
flavonoid and total antioxidant in both the extract justifies the antioxidant potential of the plants which brings about their free radicals
scavenging potential. Thus we conclude that the antioxidant activities may be due to the cumulative effect of the phytochemicals
present in the plant which genuinely designate them as free radical scavengers. |
topic |
Extracts Total phenolic compounds Total flavonoids NO radical DPPH radical hydroxyl radical superoxide anion radical |
url |
http://bioclima.ro/Balneo185.pdf |
work_keys_str_mv |
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