The combined effect of electrical stimulation and resistance isometric contraction on muscle atrophy in rat tibialis anterior muscle

Electrical stimulation has been used to prevent muscle atrophy, but this method is different in many previous studies, appropriate stimulation protocol is still not decided. Although resistance exercise has also been shown to be an effective countermeasure on muscle atrophy, almost previous studies...

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Main Authors: Naoto Fujita, Shinichiro Murakami, Takamitsu Arakawa, Akinori Miki, Hidemi Fujino
Format: Article
Language:English
Published: Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina 2011-05-01
Series:Bosnian Journal of Basic Medical Sciences
Subjects:
Online Access:http://www.bjbms.org/ojs/index.php/bjbms/article/view/2584
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spelling doaj-ee55d905e4474fff8731f9e9bdbd42162020-11-25T00:07:13ZengAssociation of Basic Medical Sciences of Federation of Bosnia and HerzegovinaBosnian Journal of Basic Medical Sciences1512-86011840-48122011-05-0111210.17305/bjbms.2011.2584344The combined effect of electrical stimulation and resistance isometric contraction on muscle atrophy in rat tibialis anterior muscleNaoto Fujita0Shinichiro Murakami1Takamitsu Arakawa2Akinori Miki3Hidemi Fujino4Department of Rehabilitation Science, Kobe University Graduate School of Health SciencesDepartment of Rehabilitation Science, Kobe University Graduate School of Health Sciences Department of Physical Therapy, Himeji Dokkyo UniversityDepartment of Rehabilitation Science, Kobe University Graduate School of Health SciencesDepartment of Rehabilitation Science, Kobe University Graduate School of Health SciencesDepartment of Rehabilitation Science, Kobe University Graduate School of Health SciencesElectrical stimulation has been used to prevent muscle atrophy, but this method is different in many previous studies, appropriate stimulation protocol is still not decided. Although resistance exercise has also been shown to be an effective countermeasure on muscle atrophy, almost previous studies carried out an electrical stimulation without resistance. It was hypothesized that electrical stimulation without resistance is insufficient to contract skeletal muscle forcefully, and the combination of electrical stimulation and forceful resistance contraction is more effective than electrical stimulation without resistance to attenuate muscle atrophy. This study investigated the combined effects of electrical stimulation and resistance isometric contraction on muscle atrophy in the rat tibialis anterior muscle. The animals were divided into control, hindlimb unloading (HU), hindlimb unloading plus electrical stimulation (ES), and hindlimb unloading plus the combination of electrical stimulation and resistance isometric contraction (ES+IC). Electrical stimulation was applied to the tibialis anterior muscle percutaneously for total 240 sec per day. In the ES+IC group, the ankle joint was fixed to produce resistance isometric contraction during electrical stimulation. After 7 days, the cross-sectional areas of each muscle fiber type in the HU group decreased. Those were prevented in the ES+IC group rather than the ES group. The expression of heat shock protein 72 was enhanced in the ES and ES+IC groups. These results indicated that although electrical stimulation is effective to prevent muscle atrophy, the combination of electrical stimulation and isometric contraction have further effect.http://www.bjbms.org/ojs/index.php/bjbms/article/view/2584muscle atrophyelectrical stimulationresistanceisometric contractionHSP72
collection DOAJ
language English
format Article
sources DOAJ
author Naoto Fujita
Shinichiro Murakami
Takamitsu Arakawa
Akinori Miki
Hidemi Fujino
spellingShingle Naoto Fujita
Shinichiro Murakami
Takamitsu Arakawa
Akinori Miki
Hidemi Fujino
The combined effect of electrical stimulation and resistance isometric contraction on muscle atrophy in rat tibialis anterior muscle
Bosnian Journal of Basic Medical Sciences
muscle atrophy
electrical stimulation
resistance
isometric contraction
HSP72
author_facet Naoto Fujita
Shinichiro Murakami
Takamitsu Arakawa
Akinori Miki
Hidemi Fujino
author_sort Naoto Fujita
title The combined effect of electrical stimulation and resistance isometric contraction on muscle atrophy in rat tibialis anterior muscle
title_short The combined effect of electrical stimulation and resistance isometric contraction on muscle atrophy in rat tibialis anterior muscle
title_full The combined effect of electrical stimulation and resistance isometric contraction on muscle atrophy in rat tibialis anterior muscle
title_fullStr The combined effect of electrical stimulation and resistance isometric contraction on muscle atrophy in rat tibialis anterior muscle
title_full_unstemmed The combined effect of electrical stimulation and resistance isometric contraction on muscle atrophy in rat tibialis anterior muscle
title_sort combined effect of electrical stimulation and resistance isometric contraction on muscle atrophy in rat tibialis anterior muscle
publisher Association of Basic Medical Sciences of Federation of Bosnia and Herzegovina
series Bosnian Journal of Basic Medical Sciences
issn 1512-8601
1840-4812
publishDate 2011-05-01
description Electrical stimulation has been used to prevent muscle atrophy, but this method is different in many previous studies, appropriate stimulation protocol is still not decided. Although resistance exercise has also been shown to be an effective countermeasure on muscle atrophy, almost previous studies carried out an electrical stimulation without resistance. It was hypothesized that electrical stimulation without resistance is insufficient to contract skeletal muscle forcefully, and the combination of electrical stimulation and forceful resistance contraction is more effective than electrical stimulation without resistance to attenuate muscle atrophy. This study investigated the combined effects of electrical stimulation and resistance isometric contraction on muscle atrophy in the rat tibialis anterior muscle. The animals were divided into control, hindlimb unloading (HU), hindlimb unloading plus electrical stimulation (ES), and hindlimb unloading plus the combination of electrical stimulation and resistance isometric contraction (ES+IC). Electrical stimulation was applied to the tibialis anterior muscle percutaneously for total 240 sec per day. In the ES+IC group, the ankle joint was fixed to produce resistance isometric contraction during electrical stimulation. After 7 days, the cross-sectional areas of each muscle fiber type in the HU group decreased. Those were prevented in the ES+IC group rather than the ES group. The expression of heat shock protein 72 was enhanced in the ES and ES+IC groups. These results indicated that although electrical stimulation is effective to prevent muscle atrophy, the combination of electrical stimulation and isometric contraction have further effect.
topic muscle atrophy
electrical stimulation
resistance
isometric contraction
HSP72
url http://www.bjbms.org/ojs/index.php/bjbms/article/view/2584
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