Expression profiles of miRNAs in giant cell tumor of bone showed miR‐187‐5p and miR‐1323 can regulate biological functions through inhibiting FRS2
Abstract Background Giant cell tumor of bone (GCTB) is considered to be a kind of borderline tumor, which has a tendency to recur and translocate. MicroRNAs are one type of small noncoding RNA, which can inhibit the translation of targeted mRNA through RNA‐induced silencing complex. Methods Microarr...
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doaj-ee62df42fd14443f9d358feb04c24fba2020-11-25T03:13:30ZengWileyCancer Medicine2045-76342020-05-01993163317310.1002/cam4.2853Expression profiles of miRNAs in giant cell tumor of bone showed miR‐187‐5p and miR‐1323 can regulate biological functions through inhibiting FRS2Yuanhan Jin0Jing Zhang1Hao Zhu2Gentao Fan3Guangxin Zhou4Department of Orthopedics Jinling Hospital Medical school of Southeast University Nanjing ChinaDepartment of Orthopedics Jinling Hospital Nanjing Medical University Nanjing ChinaDepartment of Orthopedics Jinling Hospital Nanjing University Nanjing ChinaDepartment of Orthopedics Jinling Hospital Nanjing University Nanjing ChinaDepartment of Orthopedics Jinling Hospital Nanjing University Nanjing ChinaAbstract Background Giant cell tumor of bone (GCTB) is considered to be a kind of borderline tumor, which has a tendency to recur and translocate. MicroRNAs are one type of small noncoding RNA, which can inhibit the translation of targeted mRNA through RNA‐induced silencing complex. Methods Microarray was conducted on three groups of tumor tissues and normal tissues from patients with GCTB, and results showed different expression profiles of miRNAs with Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes analysis. The functions of miR‐187‐5p and miR‐1323, which were highly expressed in GCTB, were examined by 5‐ethynyl‐2′‐deoxyuridine (EDU), transwell, and CCK8 assays. RNAhybrid et al. (RNA prediction softwares) predicted that the two microRNAs targeted fibroblast growth factor receptor substrate 2 (FRS2), which was verified by luciferase assay and rescue experiments. Results miR‐187‐5p and miR‐1323 were highly expressed in tumor tissues. They can jointly regulate the biological functions of GCTB in vitro. Luciferase assay confirmed that the two microRNAs can bind to the 3′ untranslated regions (UTR) of mRNA of FRS2. And, rescue experiments verified the relationships between the two microRNAs and FRS2. Conclusion There were some different‐expressed microRNAs between GCTB and normal tissues. miR‐187‐5p and miR‐1323 can regulate the biological functions of GCTB through influencing the expression of FRS2.https://doi.org/10.1002/cam4.2853expression profilesfunctionsGCTBmicroRNAs |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yuanhan Jin Jing Zhang Hao Zhu Gentao Fan Guangxin Zhou |
spellingShingle |
Yuanhan Jin Jing Zhang Hao Zhu Gentao Fan Guangxin Zhou Expression profiles of miRNAs in giant cell tumor of bone showed miR‐187‐5p and miR‐1323 can regulate biological functions through inhibiting FRS2 Cancer Medicine expression profiles functions GCTB microRNAs |
author_facet |
Yuanhan Jin Jing Zhang Hao Zhu Gentao Fan Guangxin Zhou |
author_sort |
Yuanhan Jin |
title |
Expression profiles of miRNAs in giant cell tumor of bone showed miR‐187‐5p and miR‐1323 can regulate biological functions through inhibiting FRS2 |
title_short |
Expression profiles of miRNAs in giant cell tumor of bone showed miR‐187‐5p and miR‐1323 can regulate biological functions through inhibiting FRS2 |
title_full |
Expression profiles of miRNAs in giant cell tumor of bone showed miR‐187‐5p and miR‐1323 can regulate biological functions through inhibiting FRS2 |
title_fullStr |
Expression profiles of miRNAs in giant cell tumor of bone showed miR‐187‐5p and miR‐1323 can regulate biological functions through inhibiting FRS2 |
title_full_unstemmed |
Expression profiles of miRNAs in giant cell tumor of bone showed miR‐187‐5p and miR‐1323 can regulate biological functions through inhibiting FRS2 |
title_sort |
expression profiles of mirnas in giant cell tumor of bone showed mir‐187‐5p and mir‐1323 can regulate biological functions through inhibiting frs2 |
publisher |
Wiley |
series |
Cancer Medicine |
issn |
2045-7634 |
publishDate |
2020-05-01 |
description |
Abstract Background Giant cell tumor of bone (GCTB) is considered to be a kind of borderline tumor, which has a tendency to recur and translocate. MicroRNAs are one type of small noncoding RNA, which can inhibit the translation of targeted mRNA through RNA‐induced silencing complex. Methods Microarray was conducted on three groups of tumor tissues and normal tissues from patients with GCTB, and results showed different expression profiles of miRNAs with Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes analysis. The functions of miR‐187‐5p and miR‐1323, which were highly expressed in GCTB, were examined by 5‐ethynyl‐2′‐deoxyuridine (EDU), transwell, and CCK8 assays. RNAhybrid et al. (RNA prediction softwares) predicted that the two microRNAs targeted fibroblast growth factor receptor substrate 2 (FRS2), which was verified by luciferase assay and rescue experiments. Results miR‐187‐5p and miR‐1323 were highly expressed in tumor tissues. They can jointly regulate the biological functions of GCTB in vitro. Luciferase assay confirmed that the two microRNAs can bind to the 3′ untranslated regions (UTR) of mRNA of FRS2. And, rescue experiments verified the relationships between the two microRNAs and FRS2. Conclusion There were some different‐expressed microRNAs between GCTB and normal tissues. miR‐187‐5p and miR‐1323 can regulate the biological functions of GCTB through influencing the expression of FRS2. |
topic |
expression profiles functions GCTB microRNAs |
url |
https://doi.org/10.1002/cam4.2853 |
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