Significance of AtMTM1 and AtMTM2 for Mitochondrial MnSOD Activation in Arabidopsis
The manganese (Mn) tracking factor for mitochondrial Mn superoxide dismutase (MnSOD) has been annotated as yMTM1 in yeast, which belongs to the mitochondrial carrier family. We confirmed that Arabidopsis AtMTM1 and AtMTM2 are functional homologs of yMTM1 as they can revive yeast MnSOD activity in yM...
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doaj-ee889e317add4623880421cb9d820b192021-08-09T16:29:26ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-08-011210.3389/fpls.2021.690064690064Significance of AtMTM1 and AtMTM2 for Mitochondrial MnSOD Activation in ArabidopsisShu-Hsuan Hu0Shu-Fan Lin1Ya-Chen Huang2Chien-Hsun Huang3Chien-Hsun Huang4Wen-Yu Kuo5Tsung-Luo Jinn6Institute of Plant Biology and Department of Life Science, National Taiwan University, Taipei, TaiwanInstitute of Plant Biology and Department of Life Science, National Taiwan University, Taipei, TaiwanInstitute of Plant Biology and Department of Life Science, National Taiwan University, Taipei, TaiwanInstitute of Plant Biology and Department of Life Science, National Taiwan University, Taipei, TaiwanInstitute of Plant Biology, Center of Evolutionary Biology, School of Life Sciences, Fudan University, Shanghai, ChinaInstitute of Plant Biology and Department of Life Science, National Taiwan University, Taipei, TaiwanInstitute of Plant Biology and Department of Life Science, National Taiwan University, Taipei, TaiwanThe manganese (Mn) tracking factor for mitochondrial Mn superoxide dismutase (MnSOD) has been annotated as yMTM1 in yeast, which belongs to the mitochondrial carrier family. We confirmed that Arabidopsis AtMTM1 and AtMTM2 are functional homologs of yMTM1 as they can revive yeast MnSOD activity in yMTM1-mutant cells. Transient expression of AtMnSOD-3xFLAG in the AtMTM1 and AtMTM2-double mutant protoplasts confirmed that AtMTM1 and AtMTM2 are required for AtMnSOD activation. Our study revealed that AtMnSOD interacts with AtMTM1 and AtMTM2 in the mitochondria. The expression levels of AtMTM1, AtMTM2, and AtMnSOD respond positively to methyl viologen (MV) and metal stress. AtMTM1 and AtMTM2 are involved in Mn and Fe homeostasis, root length, and flowering time. Transient expression of chloroplast-destined AtMnSOD revealed that an evolutionarily conserved activation mechanism, like the chloroplastic-localized MnSOD in some algae, still exists in Arabidopsis chloroplasts. This study strengthens the proposition that AtMTM1 and AtMTM2 participate in the AtMnSOD activation and ion homeostasis.https://www.frontiersin.org/articles/10.3389/fpls.2021.690064/fullmetalloenzymemitochondrial carrier familyMnSODMn transporterreactive oxygen speciessuperoxide dismutase |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Shu-Hsuan Hu Shu-Fan Lin Ya-Chen Huang Chien-Hsun Huang Chien-Hsun Huang Wen-Yu Kuo Tsung-Luo Jinn |
spellingShingle |
Shu-Hsuan Hu Shu-Fan Lin Ya-Chen Huang Chien-Hsun Huang Chien-Hsun Huang Wen-Yu Kuo Tsung-Luo Jinn Significance of AtMTM1 and AtMTM2 for Mitochondrial MnSOD Activation in Arabidopsis Frontiers in Plant Science metalloenzyme mitochondrial carrier family MnSOD Mn transporter reactive oxygen species superoxide dismutase |
author_facet |
Shu-Hsuan Hu Shu-Fan Lin Ya-Chen Huang Chien-Hsun Huang Chien-Hsun Huang Wen-Yu Kuo Tsung-Luo Jinn |
author_sort |
Shu-Hsuan Hu |
title |
Significance of AtMTM1 and AtMTM2 for Mitochondrial MnSOD Activation in Arabidopsis |
title_short |
Significance of AtMTM1 and AtMTM2 for Mitochondrial MnSOD Activation in Arabidopsis |
title_full |
Significance of AtMTM1 and AtMTM2 for Mitochondrial MnSOD Activation in Arabidopsis |
title_fullStr |
Significance of AtMTM1 and AtMTM2 for Mitochondrial MnSOD Activation in Arabidopsis |
title_full_unstemmed |
Significance of AtMTM1 and AtMTM2 for Mitochondrial MnSOD Activation in Arabidopsis |
title_sort |
significance of atmtm1 and atmtm2 for mitochondrial mnsod activation in arabidopsis |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2021-08-01 |
description |
The manganese (Mn) tracking factor for mitochondrial Mn superoxide dismutase (MnSOD) has been annotated as yMTM1 in yeast, which belongs to the mitochondrial carrier family. We confirmed that Arabidopsis AtMTM1 and AtMTM2 are functional homologs of yMTM1 as they can revive yeast MnSOD activity in yMTM1-mutant cells. Transient expression of AtMnSOD-3xFLAG in the AtMTM1 and AtMTM2-double mutant protoplasts confirmed that AtMTM1 and AtMTM2 are required for AtMnSOD activation. Our study revealed that AtMnSOD interacts with AtMTM1 and AtMTM2 in the mitochondria. The expression levels of AtMTM1, AtMTM2, and AtMnSOD respond positively to methyl viologen (MV) and metal stress. AtMTM1 and AtMTM2 are involved in Mn and Fe homeostasis, root length, and flowering time. Transient expression of chloroplast-destined AtMnSOD revealed that an evolutionarily conserved activation mechanism, like the chloroplastic-localized MnSOD in some algae, still exists in Arabidopsis chloroplasts. This study strengthens the proposition that AtMTM1 and AtMTM2 participate in the AtMnSOD activation and ion homeostasis. |
topic |
metalloenzyme mitochondrial carrier family MnSOD Mn transporter reactive oxygen species superoxide dismutase |
url |
https://www.frontiersin.org/articles/10.3389/fpls.2021.690064/full |
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