Astragaloside IV alleviates lipopolysaccharide‐induced preeclampsia‐like phenotypes via suppressing the inflammatory responses
Abstract Preeclampsia (PE) is a major cause of perinatal and maternal mortality and morbidity, which affects 2% to 8% of pregnancies in the world. The aberrant maternal inflammation and angiogenic imbalance have been demonstrated to contribute to the pathogenesis of PE. This research aimed to invest...
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doaj-ee8dd98447ff4a4291875dabfada552a2021-03-10T07:01:48ZengWileyKaohsiung Journal of Medical Sciences1607-551X2410-86502021-03-0137323624410.1002/kjm2.12313Astragaloside IV alleviates lipopolysaccharide‐induced preeclampsia‐like phenotypes via suppressing the inflammatory responsesDilihuma Tuerxun0Remila Aierken1Yan‐Mei Zhang2Ying Huang3Shuang Sui4Xiao‐Ying Li5Zulifeiya Abulikemu6Nuerbiye Dilixiati7Department of Obstestrics of People's Hospital of Xinjiang Uygur Autonomous Region Xinjiang ChinaDepartment of Obstestrics of People's Hospital of Xinjiang Uygur Autonomous Region Xinjiang ChinaDepartment of Obstestrics of People's Hospital of Xinjiang Uygur Autonomous Region Xinjiang ChinaDepartment of Obstestrics of People's Hospital of Xinjiang Uygur Autonomous Region Xinjiang ChinaDepartment of Obstestrics of People's Hospital of Xinjiang Uygur Autonomous Region Xinjiang ChinaDepartment of Obstestrics of People's Hospital of Xinjiang Uygur Autonomous Region Xinjiang ChinaDepartment of Obstestrics of People's Hospital of Xinjiang Uygur Autonomous Region Xinjiang ChinaDepartment of Obstestrics of People's Hospital of Xinjiang Uygur Autonomous Region Xinjiang ChinaAbstract Preeclampsia (PE) is a major cause of perinatal and maternal mortality and morbidity, which affects 2% to 8% of pregnancies in the world. The aberrant maternal inflammation and angiogenic imbalance have been demonstrated to contribute to the pathogenesis of PE. This research aimed to investigate the effect of Astragaloside IV (AsIV) in the treatment of PE and the underlying mechanisms. A rat PE‐like model was established by tail vein injection of lipopolysaccharide (LPS) and different doses of AsIV (40 and 80 mg/kg) were treated at the same time. Systolic blood pressure, total urine protein and urine volume were observed. Serum and placenta inflammatory cytokines were measured by ELISA kit. The mRNA and protein expression of relative genes were analyzed by qRT‐PCR and Western blotting. In PE‐like rats, there were obvious increases in systolic blood pressure, total urine protein and urine volume, which were obviously alleviated by treatment with AsIV. Serum levels of interleukin (IL)‐1β, tumor necrosis factor alpha (TNF‐α), IL‐6 and IL‐18, as well as IL‐4, IL‐10, PIGF, VEGF and sFlt‐1, were all reversed by treatment with AsIV. Meanwhile, AsIV treatment improved abnormal pregnancy outcomes, such as low litter size and low fetal weight. In addition, AsIV treatment downregulated the mRNA expression of inflammatory gene IL‐1β and IL‐6 in PE rats model, and AsIV treatment inhibited the activation of TLR‐4, NF‐κB, and sFlt‐1 in the placenta of PE rats. Our findings indicated the first evidence that AsIV alleviated PE‐like signs, and this improvement effect is possibly through inhibition of inflammation response via the TLR4/NF‐κB signaling pathway.https://doi.org/10.1002/kjm2.12313Astragaloside IVhypertensioninflammationpreeclampsia |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Dilihuma Tuerxun Remila Aierken Yan‐Mei Zhang Ying Huang Shuang Sui Xiao‐Ying Li Zulifeiya Abulikemu Nuerbiye Dilixiati |
spellingShingle |
Dilihuma Tuerxun Remila Aierken Yan‐Mei Zhang Ying Huang Shuang Sui Xiao‐Ying Li Zulifeiya Abulikemu Nuerbiye Dilixiati Astragaloside IV alleviates lipopolysaccharide‐induced preeclampsia‐like phenotypes via suppressing the inflammatory responses Kaohsiung Journal of Medical Sciences Astragaloside IV hypertension inflammation preeclampsia |
author_facet |
Dilihuma Tuerxun Remila Aierken Yan‐Mei Zhang Ying Huang Shuang Sui Xiao‐Ying Li Zulifeiya Abulikemu Nuerbiye Dilixiati |
author_sort |
Dilihuma Tuerxun |
title |
Astragaloside IV alleviates lipopolysaccharide‐induced preeclampsia‐like phenotypes via suppressing the inflammatory responses |
title_short |
Astragaloside IV alleviates lipopolysaccharide‐induced preeclampsia‐like phenotypes via suppressing the inflammatory responses |
title_full |
Astragaloside IV alleviates lipopolysaccharide‐induced preeclampsia‐like phenotypes via suppressing the inflammatory responses |
title_fullStr |
Astragaloside IV alleviates lipopolysaccharide‐induced preeclampsia‐like phenotypes via suppressing the inflammatory responses |
title_full_unstemmed |
Astragaloside IV alleviates lipopolysaccharide‐induced preeclampsia‐like phenotypes via suppressing the inflammatory responses |
title_sort |
astragaloside iv alleviates lipopolysaccharide‐induced preeclampsia‐like phenotypes via suppressing the inflammatory responses |
publisher |
Wiley |
series |
Kaohsiung Journal of Medical Sciences |
issn |
1607-551X 2410-8650 |
publishDate |
2021-03-01 |
description |
Abstract Preeclampsia (PE) is a major cause of perinatal and maternal mortality and morbidity, which affects 2% to 8% of pregnancies in the world. The aberrant maternal inflammation and angiogenic imbalance have been demonstrated to contribute to the pathogenesis of PE. This research aimed to investigate the effect of Astragaloside IV (AsIV) in the treatment of PE and the underlying mechanisms. A rat PE‐like model was established by tail vein injection of lipopolysaccharide (LPS) and different doses of AsIV (40 and 80 mg/kg) were treated at the same time. Systolic blood pressure, total urine protein and urine volume were observed. Serum and placenta inflammatory cytokines were measured by ELISA kit. The mRNA and protein expression of relative genes were analyzed by qRT‐PCR and Western blotting. In PE‐like rats, there were obvious increases in systolic blood pressure, total urine protein and urine volume, which were obviously alleviated by treatment with AsIV. Serum levels of interleukin (IL)‐1β, tumor necrosis factor alpha (TNF‐α), IL‐6 and IL‐18, as well as IL‐4, IL‐10, PIGF, VEGF and sFlt‐1, were all reversed by treatment with AsIV. Meanwhile, AsIV treatment improved abnormal pregnancy outcomes, such as low litter size and low fetal weight. In addition, AsIV treatment downregulated the mRNA expression of inflammatory gene IL‐1β and IL‐6 in PE rats model, and AsIV treatment inhibited the activation of TLR‐4, NF‐κB, and sFlt‐1 in the placenta of PE rats. Our findings indicated the first evidence that AsIV alleviated PE‐like signs, and this improvement effect is possibly through inhibition of inflammation response via the TLR4/NF‐κB signaling pathway. |
topic |
Astragaloside IV hypertension inflammation preeclampsia |
url |
https://doi.org/10.1002/kjm2.12313 |
work_keys_str_mv |
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