Effects of gold and PCL- or PLLA-coated silica nanoparticles on brain endothelial cells and the blood–brain barrier
Nanomedicine is a constantly expanding field, facilitating and improving diagnosis and treatment of diseases. As nanomaterials are foreign objects, careful evaluation of their toxicological and functional aspects prior to medical application is imperative. In this study, we aimed to determine the ef...
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doaj-ee9a12baea1c416eb35cbf142578773b2020-11-25T00:55:11ZengBeilstein-InstitutBeilstein Journal of Nanotechnology2190-42862019-04-0110194195410.3762/bjnano.10.952190-4286-10-95Effects of gold and PCL- or PLLA-coated silica nanoparticles on brain endothelial cells and the blood–brain barrierAniela Bittner0Angélique D. Ducray1Hans Rudolf Widmer2Michael H. Stoffel3Meike Mevissen4Division of Veterinary Pharmacology and Toxicology, Vetsuisse Faculty, University of Bern, Länggassstrasse 124, 3012 Bern, SwitzerlandDivision of Veterinary Pharmacology and Toxicology, Vetsuisse Faculty, University of Bern, Länggassstrasse 124, 3012 Bern, SwitzerlandDepartment of Neurosurgery, Research Unit, Inselspital, University of Bern, Freiburgstrasse 8, 3010 Bern, SwitzerlandDivision of Veterinary Anatomy, Vetsuisse Faculty, University of Bern, Länggassstrasse 120, 3012 Bern, SwitzerlandDivision of Veterinary Pharmacology and Toxicology, Vetsuisse Faculty, University of Bern, Länggassstrasse 124, 3012 Bern, SwitzerlandNanomedicine is a constantly expanding field, facilitating and improving diagnosis and treatment of diseases. As nanomaterials are foreign objects, careful evaluation of their toxicological and functional aspects prior to medical application is imperative. In this study, we aimed to determine the effects of gold and polymer-coated silica nanoparticles used in laser tissue soldering on brain endothelial cells and the blood–brain barrier using rat brain capillary endothelial cells (rBCEC4). All types of nanoparticles were taken up time-dependently by the rBCEC4 cells, albeit to a different extent, causing a time- and concentration-dependent decrease in cell viability. Nanoparticle exposure did not change cell proliferation, differentiation, nor did it induce inflammation. rBCEC4 cells showed blood–brain barrier characteristics including tight junctions. None of the nanoparticles altered the expression of tight junctions or impaired the blood–brain barrier permeability. The findings suggest that effects of these nanoparticles on the metabolic state of cells have to be further characterized before use for medical purposes.https://doi.org/10.3762/bjnano.10.95blood–brain barrierlaser tissue solderingnanomedicinenanoparticle uptakerBCEC4 cells |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Aniela Bittner Angélique D. Ducray Hans Rudolf Widmer Michael H. Stoffel Meike Mevissen |
spellingShingle |
Aniela Bittner Angélique D. Ducray Hans Rudolf Widmer Michael H. Stoffel Meike Mevissen Effects of gold and PCL- or PLLA-coated silica nanoparticles on brain endothelial cells and the blood–brain barrier Beilstein Journal of Nanotechnology blood–brain barrier laser tissue soldering nanomedicine nanoparticle uptake rBCEC4 cells |
author_facet |
Aniela Bittner Angélique D. Ducray Hans Rudolf Widmer Michael H. Stoffel Meike Mevissen |
author_sort |
Aniela Bittner |
title |
Effects of gold and PCL- or PLLA-coated silica nanoparticles on brain endothelial cells and the blood–brain barrier |
title_short |
Effects of gold and PCL- or PLLA-coated silica nanoparticles on brain endothelial cells and the blood–brain barrier |
title_full |
Effects of gold and PCL- or PLLA-coated silica nanoparticles on brain endothelial cells and the blood–brain barrier |
title_fullStr |
Effects of gold and PCL- or PLLA-coated silica nanoparticles on brain endothelial cells and the blood–brain barrier |
title_full_unstemmed |
Effects of gold and PCL- or PLLA-coated silica nanoparticles on brain endothelial cells and the blood–brain barrier |
title_sort |
effects of gold and pcl- or plla-coated silica nanoparticles on brain endothelial cells and the blood–brain barrier |
publisher |
Beilstein-Institut |
series |
Beilstein Journal of Nanotechnology |
issn |
2190-4286 |
publishDate |
2019-04-01 |
description |
Nanomedicine is a constantly expanding field, facilitating and improving diagnosis and treatment of diseases. As nanomaterials are foreign objects, careful evaluation of their toxicological and functional aspects prior to medical application is imperative. In this study, we aimed to determine the effects of gold and polymer-coated silica nanoparticles used in laser tissue soldering on brain endothelial cells and the blood–brain barrier using rat brain capillary endothelial cells (rBCEC4). All types of nanoparticles were taken up time-dependently by the rBCEC4 cells, albeit to a different extent, causing a time- and concentration-dependent decrease in cell viability. Nanoparticle exposure did not change cell proliferation, differentiation, nor did it induce inflammation. rBCEC4 cells showed blood–brain barrier characteristics including tight junctions. None of the nanoparticles altered the expression of tight junctions or impaired the blood–brain barrier permeability. The findings suggest that effects of these nanoparticles on the metabolic state of cells have to be further characterized before use for medical purposes. |
topic |
blood–brain barrier laser tissue soldering nanomedicine nanoparticle uptake rBCEC4 cells |
url |
https://doi.org/10.3762/bjnano.10.95 |
work_keys_str_mv |
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