Determination of (4E,6E,12E)-Tetradecatriene-8,10-diyne-1,3-diyl Diacetate in Rat Plasma and Tissues by HPLC-UV Method and Their Application to a Pharmacokinetic and Tissue Distribution Study
In China Atractylodis Rhizoma is widely used for the treatment of rheumatic diseases and digestive disorders. Stir-frying with wheat bran is the most common processing method. In order to clarify the influence of processing on pharmacological properties of Atractylodis Rhizoma, an investigation was...
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doaj-eeb001c953244a5ba6d9eedd25461dc82020-11-25T01:14:20ZengHindawi LimitedJournal of Analytical Methods in Chemistry2090-88652090-88732014-01-01201410.1155/2014/249061249061Determination of (4E,6E,12E)-Tetradecatriene-8,10-diyne-1,3-diyl Diacetate in Rat Plasma and Tissues by HPLC-UV Method and Their Application to a Pharmacokinetic and Tissue Distribution StudyYan Huo0Yu-Qiang Liu1Zhong-Xu Bai2Qian Cai3School of Medicine, Liaoning University of Traditional Chinese Medicine, Dalian 116600, ChinaSchool of Medicine, Liaoning University of Traditional Chinese Medicine, Dalian 116600, ChinaSchool of Medicine, Liaoning University of Traditional Chinese Medicine, Dalian 116600, ChinaSchool of Medicine, Liaoning University of Traditional Chinese Medicine, Dalian 116600, ChinaIn China Atractylodis Rhizoma is widely used for the treatment of rheumatic diseases and digestive disorders. Stir-frying with wheat bran is the most common processing method. In order to clarify the influence of processing on pharmacological properties of Atractylodis Rhizoma, an investigation was carried out to compare the pharmacokinetics and tissue distribution of typical constituent after oral administration of raw Atractylodis Rhizoma and processed ones. A simple, rapid, and sensitive high performance liquid chromatography with UV detection was developed and validated for the determination of (4E,6E,12E)-tetradecatriene-8,10-diyne-1,3-diyl diacetate in rat plasma. A chromatography was carried out on Diamonsil C18 (250 × 4.6 mm; 5 μm) analytical column, using a mobile phase which consisted of acetonitrile and 0.1% phosphoric acid water (60 : 40, v/v) at a flow rate of 1.0 mL·min−1. The wavelength was set at 336 nm. The LLOQ of (4E,6E,12E)-tetradecatriene-8,10-diyne-1,3-diyl diacetate was 0.00143 μg·mL−1. Both accuracy and precision were satisfactory. The pharmacokinetic results showed that the Tmax was 1 hour in advance and the Cmax was increased after processing. Tissue distribution showed that the highest level was in spleen. And the concentrations in the spleen were increased after stir-frying with bran.http://dx.doi.org/10.1155/2014/249061 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yan Huo Yu-Qiang Liu Zhong-Xu Bai Qian Cai |
spellingShingle |
Yan Huo Yu-Qiang Liu Zhong-Xu Bai Qian Cai Determination of (4E,6E,12E)-Tetradecatriene-8,10-diyne-1,3-diyl Diacetate in Rat Plasma and Tissues by HPLC-UV Method and Their Application to a Pharmacokinetic and Tissue Distribution Study Journal of Analytical Methods in Chemistry |
author_facet |
Yan Huo Yu-Qiang Liu Zhong-Xu Bai Qian Cai |
author_sort |
Yan Huo |
title |
Determination of (4E,6E,12E)-Tetradecatriene-8,10-diyne-1,3-diyl Diacetate in Rat Plasma and Tissues by HPLC-UV Method and Their Application to a Pharmacokinetic and Tissue Distribution Study |
title_short |
Determination of (4E,6E,12E)-Tetradecatriene-8,10-diyne-1,3-diyl Diacetate in Rat Plasma and Tissues by HPLC-UV Method and Their Application to a Pharmacokinetic and Tissue Distribution Study |
title_full |
Determination of (4E,6E,12E)-Tetradecatriene-8,10-diyne-1,3-diyl Diacetate in Rat Plasma and Tissues by HPLC-UV Method and Their Application to a Pharmacokinetic and Tissue Distribution Study |
title_fullStr |
Determination of (4E,6E,12E)-Tetradecatriene-8,10-diyne-1,3-diyl Diacetate in Rat Plasma and Tissues by HPLC-UV Method and Their Application to a Pharmacokinetic and Tissue Distribution Study |
title_full_unstemmed |
Determination of (4E,6E,12E)-Tetradecatriene-8,10-diyne-1,3-diyl Diacetate in Rat Plasma and Tissues by HPLC-UV Method and Their Application to a Pharmacokinetic and Tissue Distribution Study |
title_sort |
determination of (4e,6e,12e)-tetradecatriene-8,10-diyne-1,3-diyl diacetate in rat plasma and tissues by hplc-uv method and their application to a pharmacokinetic and tissue distribution study |
publisher |
Hindawi Limited |
series |
Journal of Analytical Methods in Chemistry |
issn |
2090-8865 2090-8873 |
publishDate |
2014-01-01 |
description |
In China Atractylodis Rhizoma is widely used for the treatment of rheumatic diseases and digestive disorders. Stir-frying with wheat bran is the most common processing method. In order to clarify the influence of processing on pharmacological properties of Atractylodis Rhizoma, an investigation was carried out to compare the pharmacokinetics and tissue distribution of typical constituent after oral administration of raw Atractylodis Rhizoma and processed ones. A simple, rapid, and sensitive high performance liquid chromatography with UV detection was developed and validated for the determination of (4E,6E,12E)-tetradecatriene-8,10-diyne-1,3-diyl diacetate in rat plasma. A chromatography was carried out on Diamonsil C18 (250 × 4.6 mm; 5 μm) analytical column, using a mobile phase which consisted of acetonitrile and 0.1% phosphoric acid water (60 : 40, v/v) at a flow rate of 1.0 mL·min−1. The wavelength was set at 336 nm. The LLOQ of (4E,6E,12E)-tetradecatriene-8,10-diyne-1,3-diyl diacetate was 0.00143 μg·mL−1. Both accuracy and precision were satisfactory. The pharmacokinetic results showed that the Tmax was 1 hour in advance and the Cmax was increased after processing. Tissue distribution showed that the highest level was in spleen. And the concentrations in the spleen were increased after stir-frying with bran. |
url |
http://dx.doi.org/10.1155/2014/249061 |
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