Establishment of PITX3-mCherry knock-in reporter human embryonic stem cell line (WAe009-A-23)

Establishment of PITX3-mCherry knock-in reporter human embryonic stem cell line (WAe009-A-23)

Pituitary homeobox 3 (Pitx3) is a key transcription factor that plays an important role in the development and maintenance of midbrain dopaminergic (mDA) neurons. Here, we established a PITX3-mCherry knock-in reporter human embryonic stem cell (hESC) line using the CRISPR/Cas9 system. PITX3-mCherry...

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Bibliographic Details
Main Authors: Sanghyun Park, Jeong-Eun Yoo, Dongjin R. Lee, Jiho Jang, Myung Soo Cho, Dae-Sung Kim, Dong-Wook Kim
Format: Article
Language:English
Published: Elsevier 2019-08-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506119301291
Description
Summary:Pituitary homeobox 3 (Pitx3) is a key transcription factor that plays an important role in the development and maintenance of midbrain dopaminergic (mDA) neurons. Here, we established a PITX3-mCherry knock-in reporter human embryonic stem cell (hESC) line using the CRISPR/Cas9 system. PITX3-mCherry hESCs maintained pluripotency marker expression and exhibited the capacity to generate all 3 germ layers and a normal karyotype. After differentiation into mDA neurons, most PITX3 immunoreactivity overlapped with the red fluorescence of mCherry. This reporter cell line may be used to study the development of mDA neurons or to enrich mDA populations for transplantation.
ISSN:1873-5061

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