Macrophage-Derived Extracellular Vesicle Promotes Hair Growth
Hair loss is a common medical problem affecting both males and females. Dermal papilla (DP) cells are the ultimate reservoir of cells with the potential of hair regeneration in hair loss patients. Here, we analyzed the role of macrophage-derived Wnts (3a and 7b) and macrophage extracellular vesicles...
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doaj-eebd8d2d805a40aa87cd321d986913e72020-11-25T02:05:23ZengMDPI AGCells2073-44092020-04-01985685610.3390/cells9040856Macrophage-Derived Extracellular Vesicle Promotes Hair GrowthRamya Lakshmi Rajendran0Prakash Gangadaran1Chang Hoon Seo2Mi Hee Kwack3Ji Min Oh4Ho Won Lee5Arunnehru Gopal6Young Kwan Sung7Shin Young Jeong8Sang-Woo Lee9Jaetae Lee10Byeong-Cheol Ahn11Department of Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu-41944, KoreaDepartment of Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu-41944, KoreaNew Drug Development Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), Daegu-41061, KoreaDepartment of Immunology, School of Medicine, Kyungpook National University, Daegu-41944, KoreaDepartment of Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu-41944, KoreaDepartment of Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu-41944, KoreaDepartment of Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu-41944, KoreaBK21 Plus KNU Biomedical Convergence Program, Department of Biomedical Science, School of Medicine, Kyungpook National University, Daegu-41944, KoreaDepartment of Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu-41944, KoreaDepartment of Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu-41944, KoreaDepartment of Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu-41944, KoreaDepartment of Nuclear Medicine, School of Medicine, Kyungpook National University, Daegu-41944, KoreaHair loss is a common medical problem affecting both males and females. Dermal papilla (DP) cells are the ultimate reservoir of cells with the potential of hair regeneration in hair loss patients. Here, we analyzed the role of macrophage-derived Wnts (3a and 7b) and macrophage extracellular vesicles (MAC-EVs) in promoting hair growth. We studied the proliferation, migration, and expression of growth factors of human-DP cells in the presence or absence of MAC-EVs. Additionally, we tested the effect of MAC-EV treatment on hair growth in a mouse model and human hair follicles. Data from western blot and flow cytometry showed that MAC-EVs were enriched with Wnt3a and Wnt7b, and more than 95% were associated with their membrane. The results suggest that Wnt proteins in MAC-EVs activate the Wnt/β-catenin signaling pathways, which leads to activation of transcription factors (<i>Axin2 and Lef1</i>). The MAC-EVs significantly enhanced the proliferation, migration, and levels of hair-inductive markers of DP cells. Additionally, MAC-EVs phosphorylated AKT and increased the levels of the survival protein Bcl-2. The DP cells treated with MAC-EVs showed increased expression of vascular endothelial growth factor (VEGF) and keratinocyte growth factor (KGF). Treatment of Balb/c mice with MAC-EVs promoted hair follicle (HF) growth in vivo and also increased hair shaft size in a short period in human HFs. Our findings suggest that MAC-EV treatment could be clinically used as a promising novel anagen inducer in the treatment of hair loss.https://www.mdpi.com/2073-4409/9/4/856macrophageextracellular vesiclesWntβ-catenin signalinghair follicledermal papilla |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ramya Lakshmi Rajendran Prakash Gangadaran Chang Hoon Seo Mi Hee Kwack Ji Min Oh Ho Won Lee Arunnehru Gopal Young Kwan Sung Shin Young Jeong Sang-Woo Lee Jaetae Lee Byeong-Cheol Ahn |
spellingShingle |
Ramya Lakshmi Rajendran Prakash Gangadaran Chang Hoon Seo Mi Hee Kwack Ji Min Oh Ho Won Lee Arunnehru Gopal Young Kwan Sung Shin Young Jeong Sang-Woo Lee Jaetae Lee Byeong-Cheol Ahn Macrophage-Derived Extracellular Vesicle Promotes Hair Growth Cells macrophage extracellular vesicles Wnt β-catenin signaling hair follicle dermal papilla |
author_facet |
Ramya Lakshmi Rajendran Prakash Gangadaran Chang Hoon Seo Mi Hee Kwack Ji Min Oh Ho Won Lee Arunnehru Gopal Young Kwan Sung Shin Young Jeong Sang-Woo Lee Jaetae Lee Byeong-Cheol Ahn |
author_sort |
Ramya Lakshmi Rajendran |
title |
Macrophage-Derived Extracellular Vesicle Promotes Hair Growth |
title_short |
Macrophage-Derived Extracellular Vesicle Promotes Hair Growth |
title_full |
Macrophage-Derived Extracellular Vesicle Promotes Hair Growth |
title_fullStr |
Macrophage-Derived Extracellular Vesicle Promotes Hair Growth |
title_full_unstemmed |
Macrophage-Derived Extracellular Vesicle Promotes Hair Growth |
title_sort |
macrophage-derived extracellular vesicle promotes hair growth |
publisher |
MDPI AG |
series |
Cells |
issn |
2073-4409 |
publishDate |
2020-04-01 |
description |
Hair loss is a common medical problem affecting both males and females. Dermal papilla (DP) cells are the ultimate reservoir of cells with the potential of hair regeneration in hair loss patients. Here, we analyzed the role of macrophage-derived Wnts (3a and 7b) and macrophage extracellular vesicles (MAC-EVs) in promoting hair growth. We studied the proliferation, migration, and expression of growth factors of human-DP cells in the presence or absence of MAC-EVs. Additionally, we tested the effect of MAC-EV treatment on hair growth in a mouse model and human hair follicles. Data from western blot and flow cytometry showed that MAC-EVs were enriched with Wnt3a and Wnt7b, and more than 95% were associated with their membrane. The results suggest that Wnt proteins in MAC-EVs activate the Wnt/β-catenin signaling pathways, which leads to activation of transcription factors (<i>Axin2 and Lef1</i>). The MAC-EVs significantly enhanced the proliferation, migration, and levels of hair-inductive markers of DP cells. Additionally, MAC-EVs phosphorylated AKT and increased the levels of the survival protein Bcl-2. The DP cells treated with MAC-EVs showed increased expression of vascular endothelial growth factor (VEGF) and keratinocyte growth factor (KGF). Treatment of Balb/c mice with MAC-EVs promoted hair follicle (HF) growth in vivo and also increased hair shaft size in a short period in human HFs. Our findings suggest that MAC-EV treatment could be clinically used as a promising novel anagen inducer in the treatment of hair loss. |
topic |
macrophage extracellular vesicles Wnt β-catenin signaling hair follicle dermal papilla |
url |
https://www.mdpi.com/2073-4409/9/4/856 |
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