Lipid profile dataset of optogenetics induced optic nerve regeneration
The optic nerve transfers visual information from the retina to the brain through the axons of retinal ganglion cells (RGCs). In adult mammals, optic nerve injuries and progressive degenerative diseases lead to the irreversible loss of RGCs, resulting in vision loss and blindness. Optogenetic models...
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doaj-eeca5b1f188c46c0be1009c2dc3d60eb2020-11-25T03:11:35ZengElsevierData in Brief2352-34092020-08-0131106001Lipid profile dataset of optogenetics induced optic nerve regenerationJennifer Arcuri0Yuan Liu1Richard K. Lee2Sanjoy K. Bhattacharya3Bascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL 33136, United States; Miami Integrative Metabolomics Research Center, Miami, FL 33136, United States; Molecular Cellular Pharmacology Graduate Program, University of Miami, Miami, FL 33136, United StatesBascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL 33136, United StatesBascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL 33136, United States; Miami Integrative Metabolomics Research Center, Miami, FL 33136, United States; Corresponding authors.Bascom Palmer Eye Institute, Miller School of Medicine at University of Miami, Miami, FL 33136, United States; Miami Integrative Metabolomics Research Center, Miami, FL 33136, United States; Molecular Cellular Pharmacology Graduate Program, University of Miami, Miami, FL 33136, United States; Corresponding authors.The optic nerve transfers visual information from the retina to the brain through the axons of retinal ganglion cells (RGCs). In adult mammals, optic nerve injuries and progressive degenerative diseases lead to the irreversible loss of RGCs, resulting in vision loss and blindness. Optogenetic models have proved useful in manipulating the growth of RGCs through expression and stimulation of channelrhodopsins (Chr2) in RGCs using the RGC-specific thy-1 promoter. Using transgenic Chr2 mouse (Thy1-ChR2-EYFP) as a model of regeneration, we profile the lipid changes which occur after traumatic optic nerve crush, light stimulation and forced RGC axonal growth. Thy1-ChR2-EYFP and control (C57BL/6) mice were divided in four groups each – 1) no crush and no stimulation, 2) no crush with stimulation, 3) crush and without stimulation, and 4) crush with stimulation. After euthanasia, the optic nerves were collected for lipidomic analysis. The Bligh and Dyer method was used for lipid extraction, followed by mass spectrometry lipid profiling with a Q-Exactive Orbitrap Liquid Chromatography-Mass Spectrometer (LC MS-MS). The raw scans were analysed with LipidSearch 4.1.3 and the statistical analysis was conducted through Metaboanalyst 4.0. This data is available at Metabolomics Workbench, study ID ST001381: [https://www.metabolomicsworkbench.org/data/DRCCMetadata.php?Mode=Study&StudyID=ST001381&StudyType=MS&ResultType=5].http://www.sciencedirect.com/science/article/pii/S2352340920308957Optic nerve crushOptogeneticsLipidsRegeneration |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jennifer Arcuri Yuan Liu Richard K. Lee Sanjoy K. Bhattacharya |
spellingShingle |
Jennifer Arcuri Yuan Liu Richard K. Lee Sanjoy K. Bhattacharya Lipid profile dataset of optogenetics induced optic nerve regeneration Data in Brief Optic nerve crush Optogenetics Lipids Regeneration |
author_facet |
Jennifer Arcuri Yuan Liu Richard K. Lee Sanjoy K. Bhattacharya |
author_sort |
Jennifer Arcuri |
title |
Lipid profile dataset of optogenetics induced optic nerve regeneration |
title_short |
Lipid profile dataset of optogenetics induced optic nerve regeneration |
title_full |
Lipid profile dataset of optogenetics induced optic nerve regeneration |
title_fullStr |
Lipid profile dataset of optogenetics induced optic nerve regeneration |
title_full_unstemmed |
Lipid profile dataset of optogenetics induced optic nerve regeneration |
title_sort |
lipid profile dataset of optogenetics induced optic nerve regeneration |
publisher |
Elsevier |
series |
Data in Brief |
issn |
2352-3409 |
publishDate |
2020-08-01 |
description |
The optic nerve transfers visual information from the retina to the brain through the axons of retinal ganglion cells (RGCs). In adult mammals, optic nerve injuries and progressive degenerative diseases lead to the irreversible loss of RGCs, resulting in vision loss and blindness. Optogenetic models have proved useful in manipulating the growth of RGCs through expression and stimulation of channelrhodopsins (Chr2) in RGCs using the RGC-specific thy-1 promoter. Using transgenic Chr2 mouse (Thy1-ChR2-EYFP) as a model of regeneration, we profile the lipid changes which occur after traumatic optic nerve crush, light stimulation and forced RGC axonal growth. Thy1-ChR2-EYFP and control (C57BL/6) mice were divided in four groups each – 1) no crush and no stimulation, 2) no crush with stimulation, 3) crush and without stimulation, and 4) crush with stimulation. After euthanasia, the optic nerves were collected for lipidomic analysis. The Bligh and Dyer method was used for lipid extraction, followed by mass spectrometry lipid profiling with a Q-Exactive Orbitrap Liquid Chromatography-Mass Spectrometer (LC MS-MS). The raw scans were analysed with LipidSearch 4.1.3 and the statistical analysis was conducted through Metaboanalyst 4.0. This data is available at Metabolomics Workbench, study ID ST001381: [https://www.metabolomicsworkbench.org/data/DRCCMetadata.php?Mode=Study&StudyID=ST001381&StudyType=MS&ResultType=5]. |
topic |
Optic nerve crush Optogenetics Lipids Regeneration |
url |
http://www.sciencedirect.com/science/article/pii/S2352340920308957 |
work_keys_str_mv |
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