Melatonin may prevent or reverse polycystic ovary syndrome in rats
SUMMARY OBJECTIVE To evaluate the ovarian effects of melatonin (Mel) in a rat model of polycystic-ovary-syndrome (PCOS) before and after permanent estrus induction. METHODS Thirty-two adult-female rats with regular estrous cycle were equally divided into four groups: 1) GCtrl – at estrous phase....
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doaj-eed02056d0d34f73a4e82d517e24c1e72020-11-25T02:39:50ZengAssociação Médica BrasileiraRevista da Associação Médica Brasileira1806-92826571008101410.1590/1806-9282.65.7.1008S0104-42302019000701008Melatonin may prevent or reverse polycystic ovary syndrome in ratsLeonardo Augusto LombardiLeandro Sabará de MattosRicardo Santos SimõesRinaldo Florencio-SilvaGisela Rodrigues da Silva SassoAdriana Aparecida Ferraz CarbonelManuel Jesus SimõesEdmund Chada BaracatJosé Maria Soares JrSUMMARY OBJECTIVE To evaluate the ovarian effects of melatonin (Mel) in a rat model of polycystic-ovary-syndrome (PCOS) before and after permanent estrus induction. METHODS Thirty-two adult-female rats with regular estrous cycle were equally divided into four groups: 1) GCtrl – at estrous phase. 2) GPCOS - at permanent-estrous phase. 3) GMel1 – treated for 60 days with Mel (0.4 mg/Kg) during permanent estrus induction and 4) GMel2 – rats with PCOS and treated for 60 days with Mel. After that, the animals were euthanized, and the ovaries were removed and processed for paraffin embedding. Sections were stained with H.E. for histomorphometry or subjected to immunohistochemistry for Ki-67 and cleaved caspase-3 (Casp-3) detections. RESULTS The GPCOS showed lack of corpus luteum and several ovarian cysts, as well as interstitial-like cells. The presence of corpus luteum and a significant increase in primary and antral follicles were observed in Mel-treated groups, which also showed a decrease in the number of ovarian cysts and in the area occupied by interstitial-like cells. These results were more evident in GMel1. The percentage of Ki-67-positive cells was significantly higher in the Mel-treated groups, mainly in the GMel2, as compared to GPCOS. On the other hand, the percentage of Casp-3-positive cells was significantly lower in granulosa cells of GMel1, whereas it was significantly higher in the interstitial-like cells of GMel2, in comparison to GPCOS. CONCLUSION Melatonin administration prevents the permanent estrus state in the PCOS rat model. This effect is more efficient when melatonin is administered before permanent estrus induction.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302019000701008&lng=en&tlng=enOvárioMelatoninaSíndrome do ovário policísticoCiclo estralRatos |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Leonardo Augusto Lombardi Leandro Sabará de Mattos Ricardo Santos Simões Rinaldo Florencio-Silva Gisela Rodrigues da Silva Sasso Adriana Aparecida Ferraz Carbonel Manuel Jesus Simões Edmund Chada Baracat José Maria Soares Jr |
spellingShingle |
Leonardo Augusto Lombardi Leandro Sabará de Mattos Ricardo Santos Simões Rinaldo Florencio-Silva Gisela Rodrigues da Silva Sasso Adriana Aparecida Ferraz Carbonel Manuel Jesus Simões Edmund Chada Baracat José Maria Soares Jr Melatonin may prevent or reverse polycystic ovary syndrome in rats Revista da Associação Médica Brasileira Ovário Melatonina Síndrome do ovário policístico Ciclo estral Ratos |
author_facet |
Leonardo Augusto Lombardi Leandro Sabará de Mattos Ricardo Santos Simões Rinaldo Florencio-Silva Gisela Rodrigues da Silva Sasso Adriana Aparecida Ferraz Carbonel Manuel Jesus Simões Edmund Chada Baracat José Maria Soares Jr |
author_sort |
Leonardo Augusto Lombardi |
title |
Melatonin may prevent or reverse polycystic ovary syndrome in rats |
title_short |
Melatonin may prevent or reverse polycystic ovary syndrome in rats |
title_full |
Melatonin may prevent or reverse polycystic ovary syndrome in rats |
title_fullStr |
Melatonin may prevent or reverse polycystic ovary syndrome in rats |
title_full_unstemmed |
Melatonin may prevent or reverse polycystic ovary syndrome in rats |
title_sort |
melatonin may prevent or reverse polycystic ovary syndrome in rats |
publisher |
Associação Médica Brasileira |
series |
Revista da Associação Médica Brasileira |
issn |
1806-9282 |
description |
SUMMARY OBJECTIVE To evaluate the ovarian effects of melatonin (Mel) in a rat model of polycystic-ovary-syndrome (PCOS) before and after permanent estrus induction. METHODS Thirty-two adult-female rats with regular estrous cycle were equally divided into four groups: 1) GCtrl – at estrous phase. 2) GPCOS - at permanent-estrous phase. 3) GMel1 – treated for 60 days with Mel (0.4 mg/Kg) during permanent estrus induction and 4) GMel2 – rats with PCOS and treated for 60 days with Mel. After that, the animals were euthanized, and the ovaries were removed and processed for paraffin embedding. Sections were stained with H.E. for histomorphometry or subjected to immunohistochemistry for Ki-67 and cleaved caspase-3 (Casp-3) detections. RESULTS The GPCOS showed lack of corpus luteum and several ovarian cysts, as well as interstitial-like cells. The presence of corpus luteum and a significant increase in primary and antral follicles were observed in Mel-treated groups, which also showed a decrease in the number of ovarian cysts and in the area occupied by interstitial-like cells. These results were more evident in GMel1. The percentage of Ki-67-positive cells was significantly higher in the Mel-treated groups, mainly in the GMel2, as compared to GPCOS. On the other hand, the percentage of Casp-3-positive cells was significantly lower in granulosa cells of GMel1, whereas it was significantly higher in the interstitial-like cells of GMel2, in comparison to GPCOS. CONCLUSION Melatonin administration prevents the permanent estrus state in the PCOS rat model. This effect is more efficient when melatonin is administered before permanent estrus induction. |
topic |
Ovário Melatonina Síndrome do ovário policístico Ciclo estral Ratos |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-42302019000701008&lng=en&tlng=en |
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