Production of L-asparaginase by filamentous fungi

• Main Authors: Maria Inez de Moura Sarquis, Edna Maria Morais Oliveira, Alberdan Silva Santos, Gisela Lara da Costa
• Format: Article
• Language: English
• Published: Instituto Oswaldo Cruz, Ministério da Saúde 2004-08-01
• Series: Memórias do Instituto Oswaldo Cruz.
• Subjects: filamentous fungi; production of L-asparaginase; Aspergillus tamarii; Aspergillus terreus
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000500005

L-asparaginase production was investigated in the filamentous fungi Aspergillus tamarii and Aspergillus terreus. The fungi were cultivated in medium containing different nitrogen sources. A. terreus showed the highest L-asparaginase (activity) production level (58 U/L) when cultivated in a 2% proline medium. Both fungi presented the lowest level of L-asparaginase production in the presence of glutamine and urea as nitrogen sources. These results suggest that L-asparaginase production by of filamentous fungi is under nitrogen regulation.