Identification of Uncommon Pathogenic Bacteria by MALDI-TOF Mass Spectrometry Using a Custom Library of Siriraj Hospital

Objective: Matrix Assisted Laser Desorption Ionization Time-Of-Flight mass spectrometry (MALDI-TOF MS) is a rapid and cost-effective technique that serves as an alternative method for bacterial identification in microbiology laboratories. This study aimed to determine the performance of MALDI-TOF M...

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Main Authors:	Kanda Ekcharoenkul, Popchai Ngamskulrungroj, Keerati Joyjamras, Amornrut Leelaporn, Azian Harun, Pattarachai Kiratisin
Format:	Article
Language:	English
Published:	Mahidol University 2018-06-01
Series:	Siriraj Medical Journal
Online Access:	https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/127490

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spelling	doaj-eeff13c9f1a844c3a7a495b6002371ca2021-08-13T09:49:09ZengMahidol UniversitySiriraj Medical Journal2228-80822018-06-01702Identification of Uncommon Pathogenic Bacteria by MALDI-TOF Mass Spectrometry Using a Custom Library of Siriraj HospitalKanda Ekcharoenkul0Popchai Ngamskulrungroj1Keerati Joyjamras2Amornrut Leelaporn3Azian Harun4Pattarachai Kiratisin5Departments of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700Departments of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700Departments of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700Departments of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700Department of Medical Microbiology and Parasitology, School of Medical Sciences, University Sains Malaysia, 16150 Kubang Kerian, KelantanDepartments of Microbiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700 Objective: Matrix Assisted Laser Desorption Ionization Time-Of-Flight mass spectrometry (MALDI-TOF MS) is a rapid and cost-effective technique that serves as an alternative method for bacterial identification in microbiology laboratories. This study aimed to determine the performance of MALDI-TOF MS for identifying uncommon and/ or fastidious bacteria comparing with automated identification method. Methods: We observed the performances of original and extended databases after adding of in-house MALDI-TOF MS database for a total of 54 isolates belonging to 16 genera of uncommon and/or fastidious bacteria. Results: The extended database improved identification of these bacteria significantly except for Listeria monocytogenes and Elizabethkingia meningoseptica. Conclusion: This study provides a possibility for identification of uncommon and/or fastidious bacteria by MALDITOF MS method. https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/127490
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Kanda Ekcharoenkul Popchai Ngamskulrungroj Keerati Joyjamras Amornrut Leelaporn Azian Harun Pattarachai Kiratisin
spellingShingle	Kanda Ekcharoenkul Popchai Ngamskulrungroj Keerati Joyjamras Amornrut Leelaporn Azian Harun Pattarachai Kiratisin Identification of Uncommon Pathogenic Bacteria by MALDI-TOF Mass Spectrometry Using a Custom Library of Siriraj Hospital Siriraj Medical Journal
author_facet	Kanda Ekcharoenkul Popchai Ngamskulrungroj Keerati Joyjamras Amornrut Leelaporn Azian Harun Pattarachai Kiratisin
author_sort	Kanda Ekcharoenkul
title	Identification of Uncommon Pathogenic Bacteria by MALDI-TOF Mass Spectrometry Using a Custom Library of Siriraj Hospital
title_short	Identification of Uncommon Pathogenic Bacteria by MALDI-TOF Mass Spectrometry Using a Custom Library of Siriraj Hospital
title_full	Identification of Uncommon Pathogenic Bacteria by MALDI-TOF Mass Spectrometry Using a Custom Library of Siriraj Hospital
title_fullStr	Identification of Uncommon Pathogenic Bacteria by MALDI-TOF Mass Spectrometry Using a Custom Library of Siriraj Hospital
title_full_unstemmed	Identification of Uncommon Pathogenic Bacteria by MALDI-TOF Mass Spectrometry Using a Custom Library of Siriraj Hospital
title_sort	identification of uncommon pathogenic bacteria by maldi-tof mass spectrometry using a custom library of siriraj hospital
publisher	Mahidol University
series	Siriraj Medical Journal
issn	2228-8082
publishDate	2018-06-01
description	Objective: Matrix Assisted Laser Desorption Ionization Time-Of-Flight mass spectrometry (MALDI-TOF MS) is a rapid and cost-effective technique that serves as an alternative method for bacterial identification in microbiology laboratories. This study aimed to determine the performance of MALDI-TOF MS for identifying uncommon and/ or fastidious bacteria comparing with automated identification method. Methods: We observed the performances of original and extended databases after adding of in-house MALDI-TOF MS database for a total of 54 isolates belonging to 16 genera of uncommon and/or fastidious bacteria. Results: The extended database improved identification of these bacteria significantly except for Listeria monocytogenes and Elizabethkingia meningoseptica. Conclusion: This study provides a possibility for identification of uncommon and/or fastidious bacteria by MALDITOF MS method.
url	https://he02.tci-thaijo.org/index.php/sirirajmedj/article/view/127490
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Identification of Uncommon Pathogenic Bacteria by MALDI-TOF Mass Spectrometry Using a Custom Library of Siriraj Hospital

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