Comprehensive proteome profiling in Aedes albopictus to decipher Wolbachia-arbovirus interference phenomenon
Abstract Background Aedes albopictus is a vector of arboviruses that cause severe diseases in humans such as Chikungunya, Dengue and Zika fevers. The vector competence of Ae. albopictus varies depending on the mosquito population involved and the virus transmitted. Wolbachia infection status in beli...
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doaj-ef05d341ba2d4343b989a611ba3f58cc2020-11-24T22:13:29ZengBMCBMC Genomics1471-21642017-08-0118111410.1186/s12864-017-3985-yComprehensive proteome profiling in Aedes albopictus to decipher Wolbachia-arbovirus interference phenomenonYoann Saucereau0Claire Valiente Moro1Cindy Dieryckx2Jean-William Dupuy3Florence-Hélène Tran4Vincent Girard5Patrick Potier6Patrick Mavingui7Université de LyonUniversité de LyonLaboratoire Mixte UMR 5240, Plateforme de Protéomique, CNRSCentre de Génomique Fonctionnelle, Plateforme Protéome, Université BordeauxUniversité de LyonLaboratoire Mixte UMR 5240, Plateforme de Protéomique, CNRSUniversité de LyonUniversité de LyonAbstract Background Aedes albopictus is a vector of arboviruses that cause severe diseases in humans such as Chikungunya, Dengue and Zika fevers. The vector competence of Ae. albopictus varies depending on the mosquito population involved and the virus transmitted. Wolbachia infection status in believed to be among key elements that determine viral transmission efficiency. Little is known about the cellular functions mobilized in Ae. albopictus during co-infection by Wolbachia and a given arbovirus. To decipher this tripartite interaction at the molecular level, we performed a proteome analysis in Ae. albopictus C6/36 cells mono-infected by Wolbachia wAlbB strain or Chikungunya virus (CHIKV), and bi-infected. Results We first confirmed significant inhibition of CHIKV by Wolbachia. Using two-dimensional gel electrophoresis followed by nano liquid chromatography coupled with tandem mass spectrometry, we identified 600 unique differentially expressed proteins mostly related to glycolysis, translation and protein metabolism. Wolbachia infection had greater impact on cellular functions than CHIKV infection, inducing either up or down-regulation of proteins associated with metabolic processes such as glycolysis and ATP metabolism, or structural glycoproteins and capsid proteins in the case of bi-infection with CHIKV. CHIKV infection inhibited expression of proteins linked with the processes of transcription, translation, lipid storage and miRNA pathways. Conclusions The results of our proteome profiling have provided new insights into the molecular pathways involved in tripartite Ae. albopictus-Wolbachia-CHIKV interaction and may help defining targets for the better implementation of Wolbachia-based strategies for disease transmission control.http://link.springer.com/article/10.1186/s12864-017-3985-yAedes albopictusWolbachiaViral inhibitionProteomeGlycolysisMetabolism |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yoann Saucereau Claire Valiente Moro Cindy Dieryckx Jean-William Dupuy Florence-Hélène Tran Vincent Girard Patrick Potier Patrick Mavingui |
spellingShingle |
Yoann Saucereau Claire Valiente Moro Cindy Dieryckx Jean-William Dupuy Florence-Hélène Tran Vincent Girard Patrick Potier Patrick Mavingui Comprehensive proteome profiling in Aedes albopictus to decipher Wolbachia-arbovirus interference phenomenon BMC Genomics Aedes albopictus Wolbachia Viral inhibition Proteome Glycolysis Metabolism |
author_facet |
Yoann Saucereau Claire Valiente Moro Cindy Dieryckx Jean-William Dupuy Florence-Hélène Tran Vincent Girard Patrick Potier Patrick Mavingui |
author_sort |
Yoann Saucereau |
title |
Comprehensive proteome profiling in Aedes albopictus to decipher Wolbachia-arbovirus interference phenomenon |
title_short |
Comprehensive proteome profiling in Aedes albopictus to decipher Wolbachia-arbovirus interference phenomenon |
title_full |
Comprehensive proteome profiling in Aedes albopictus to decipher Wolbachia-arbovirus interference phenomenon |
title_fullStr |
Comprehensive proteome profiling in Aedes albopictus to decipher Wolbachia-arbovirus interference phenomenon |
title_full_unstemmed |
Comprehensive proteome profiling in Aedes albopictus to decipher Wolbachia-arbovirus interference phenomenon |
title_sort |
comprehensive proteome profiling in aedes albopictus to decipher wolbachia-arbovirus interference phenomenon |
publisher |
BMC |
series |
BMC Genomics |
issn |
1471-2164 |
publishDate |
2017-08-01 |
description |
Abstract Background Aedes albopictus is a vector of arboviruses that cause severe diseases in humans such as Chikungunya, Dengue and Zika fevers. The vector competence of Ae. albopictus varies depending on the mosquito population involved and the virus transmitted. Wolbachia infection status in believed to be among key elements that determine viral transmission efficiency. Little is known about the cellular functions mobilized in Ae. albopictus during co-infection by Wolbachia and a given arbovirus. To decipher this tripartite interaction at the molecular level, we performed a proteome analysis in Ae. albopictus C6/36 cells mono-infected by Wolbachia wAlbB strain or Chikungunya virus (CHIKV), and bi-infected. Results We first confirmed significant inhibition of CHIKV by Wolbachia. Using two-dimensional gel electrophoresis followed by nano liquid chromatography coupled with tandem mass spectrometry, we identified 600 unique differentially expressed proteins mostly related to glycolysis, translation and protein metabolism. Wolbachia infection had greater impact on cellular functions than CHIKV infection, inducing either up or down-regulation of proteins associated with metabolic processes such as glycolysis and ATP metabolism, or structural glycoproteins and capsid proteins in the case of bi-infection with CHIKV. CHIKV infection inhibited expression of proteins linked with the processes of transcription, translation, lipid storage and miRNA pathways. Conclusions The results of our proteome profiling have provided new insights into the molecular pathways involved in tripartite Ae. albopictus-Wolbachia-CHIKV interaction and may help defining targets for the better implementation of Wolbachia-based strategies for disease transmission control. |
topic |
Aedes albopictus Wolbachia Viral inhibition Proteome Glycolysis Metabolism |
url |
http://link.springer.com/article/10.1186/s12864-017-3985-y |
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