Visualizing the metazoan proliferation-quiescence decision in vivo
Cell proliferation and quiescence are intimately coordinated during metazoan development. Here, we adapt a cyclin-dependent kinase (CDK) sensor to uncouple these key events of the cell cycle in Caenorhabditis elegans and zebrafish through live-cell imaging. The CDK sensor consists of a fluorescently...
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doaj-ef13a2875ea8461db4c27f46e2101a882021-05-05T21:53:13ZengeLife Sciences Publications LtdeLife2050-084X2020-12-01910.7554/eLife.63265Visualizing the metazoan proliferation-quiescence decision in vivoRebecca C Adikes0https://orcid.org/0000-0002-7526-8701Abraham Q Kohrman1https://orcid.org/0000-0002-3726-1090Michael A Q Martinez2https://orcid.org/0000-0003-1178-7139Nicholas J Palmisano3https://orcid.org/0000-0002-7992-4462Jayson J Smith4https://orcid.org/0000-0001-8525-7873Taylor N Medwig-Kinney5https://orcid.org/0000-0001-7989-3291Mingwei Min6https://orcid.org/0000-0002-9050-5330Maria D Sallee7Ononnah B Ahmed8Nuri Kim9Simeiyun Liu10Robert D Morabito11Nicholas Weeks12Qinyun Zhao13Wan Zhang14Jessica L Feldman15https://orcid.org/0000-0002-5210-5045Michalis Barkoulas16https://orcid.org/0000-0003-1974-7668Ariel M Pani17Sabrina L Spencer18https://orcid.org/0000-0002-5798-3007Benjamin L Martin19https://orcid.org/0000-0001-5474-4492David Q Matus20https://orcid.org/0000-0002-1570-5025Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United StatesDepartment of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United StatesDepartment of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United StatesDepartment of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United StatesDepartment of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United StatesDepartment of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United StatesDepartment of Biochemistry and BioFrontiers Institute, University of Colorado Boulder, Boulder, United StatesDepartment of Biology, Stanford University, Stanford, United StatesDepartment of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United StatesDepartment of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United StatesDepartment of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United StatesDepartment of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United StatesDepartment of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United StatesDepartment of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United StatesDepartment of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United StatesDepartment of Biology, Stanford University, Stanford, United StatesDepartment of Life Sciences, Imperial College, London, United KingdomDepartment of Biology, University of Virginia, Charlottesville, United StatesDepartment of Biochemistry and BioFrontiers Institute, University of Colorado Boulder, Boulder, United StatesDepartment of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United StatesDepartment of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, United StatesCell proliferation and quiescence are intimately coordinated during metazoan development. Here, we adapt a cyclin-dependent kinase (CDK) sensor to uncouple these key events of the cell cycle in Caenorhabditis elegans and zebrafish through live-cell imaging. The CDK sensor consists of a fluorescently tagged CDK substrate that steadily translocates from the nucleus to the cytoplasm in response to increasing CDK activity and consequent sensor phosphorylation. We show that the CDK sensor can distinguish cycling cells in G1 from quiescent cells in G0, revealing a possible commitment point and a cryptic stochasticity in an otherwise invariant C. elegans cell lineage. Finally, we derive a predictive model of future proliferation behavior in C. elegans based on a snapshot of CDK activity in newly born cells. Thus, we introduce a live-cell imaging tool to facilitate in vivo studies of cell-cycle control in a wide-range of developmental contexts.https://elifesciences.org/articles/63265cell cyclequiescencecell proliferationG1/G0CDK sensor |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Rebecca C Adikes Abraham Q Kohrman Michael A Q Martinez Nicholas J Palmisano Jayson J Smith Taylor N Medwig-Kinney Mingwei Min Maria D Sallee Ononnah B Ahmed Nuri Kim Simeiyun Liu Robert D Morabito Nicholas Weeks Qinyun Zhao Wan Zhang Jessica L Feldman Michalis Barkoulas Ariel M Pani Sabrina L Spencer Benjamin L Martin David Q Matus |
spellingShingle |
Rebecca C Adikes Abraham Q Kohrman Michael A Q Martinez Nicholas J Palmisano Jayson J Smith Taylor N Medwig-Kinney Mingwei Min Maria D Sallee Ononnah B Ahmed Nuri Kim Simeiyun Liu Robert D Morabito Nicholas Weeks Qinyun Zhao Wan Zhang Jessica L Feldman Michalis Barkoulas Ariel M Pani Sabrina L Spencer Benjamin L Martin David Q Matus Visualizing the metazoan proliferation-quiescence decision in vivo eLife cell cycle quiescence cell proliferation G1/G0 CDK sensor |
author_facet |
Rebecca C Adikes Abraham Q Kohrman Michael A Q Martinez Nicholas J Palmisano Jayson J Smith Taylor N Medwig-Kinney Mingwei Min Maria D Sallee Ononnah B Ahmed Nuri Kim Simeiyun Liu Robert D Morabito Nicholas Weeks Qinyun Zhao Wan Zhang Jessica L Feldman Michalis Barkoulas Ariel M Pani Sabrina L Spencer Benjamin L Martin David Q Matus |
author_sort |
Rebecca C Adikes |
title |
Visualizing the metazoan proliferation-quiescence decision in vivo |
title_short |
Visualizing the metazoan proliferation-quiescence decision in vivo |
title_full |
Visualizing the metazoan proliferation-quiescence decision in vivo |
title_fullStr |
Visualizing the metazoan proliferation-quiescence decision in vivo |
title_full_unstemmed |
Visualizing the metazoan proliferation-quiescence decision in vivo |
title_sort |
visualizing the metazoan proliferation-quiescence decision in vivo |
publisher |
eLife Sciences Publications Ltd |
series |
eLife |
issn |
2050-084X |
publishDate |
2020-12-01 |
description |
Cell proliferation and quiescence are intimately coordinated during metazoan development. Here, we adapt a cyclin-dependent kinase (CDK) sensor to uncouple these key events of the cell cycle in Caenorhabditis elegans and zebrafish through live-cell imaging. The CDK sensor consists of a fluorescently tagged CDK substrate that steadily translocates from the nucleus to the cytoplasm in response to increasing CDK activity and consequent sensor phosphorylation. We show that the CDK sensor can distinguish cycling cells in G1 from quiescent cells in G0, revealing a possible commitment point and a cryptic stochasticity in an otherwise invariant C. elegans cell lineage. Finally, we derive a predictive model of future proliferation behavior in C. elegans based on a snapshot of CDK activity in newly born cells. Thus, we introduce a live-cell imaging tool to facilitate in vivo studies of cell-cycle control in a wide-range of developmental contexts. |
topic |
cell cycle quiescence cell proliferation G1/G0 CDK sensor |
url |
https://elifesciences.org/articles/63265 |
work_keys_str_mv |
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