Serum amyloid A and clusterin as potential predictive biomarkers for severe hand, foot and mouth disease by 2D-DIGE proteomics analysis.
Hand, foot, and mouth disease (HFMD) affects more than one million children, is responsible for several hundred child deaths every year in China and is the cause of widespread concerns in society. Only a small fraction of HFMD cases will develop further into severe HFMD with neurologic complications...
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doaj-ef26e40fcf7d4cb4bd980911d5073c162020-11-25T01:26:18ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0199e10881610.1371/journal.pone.0108816Serum amyloid A and clusterin as potential predictive biomarkers for severe hand, foot and mouth disease by 2D-DIGE proteomics analysis.Jianjun LiuPeiwu HuangYaqing HeWen-Xu HongXiaohu RenXifei YangYanxia HeWenjian WangRenli ZhangHong YangZhiguang ZhaoHaiyan HuangLong ChenDejian ZhaoHuixia XianFang YangDongli MaLinqing YangYundong YinLi ZhouXiaozhen ChenJinquan ChengHand, foot, and mouth disease (HFMD) affects more than one million children, is responsible for several hundred child deaths every year in China and is the cause of widespread concerns in society. Only a small fraction of HFMD cases will develop further into severe HFMD with neurologic complications. A timely and accurate diagnosis of severe HFMD is essential for assessing the risk of progression and planning the appropriate treatment. Human serum can reflect the physiological or pathological states, which is expected to be an excellent source of disease-specific biomarkers. In the present study, a comparative serological proteome analysis between severe HFMD patients and healthy controls was performed via a two-dimensional difference gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) strategy. Fifteen proteins were identified as differentially expressed in the sera of the severe HFMD patients compared with the controls. The identified proteins were classified into different groups according to their molecular functions, biological processes, protein classes and physiological pathways by bioinformatics analysis. The up-regulations of two identified proteins, serum amyloid A (SAA) and clusterin (CLU), were confirmed in the sera of the HFMD patients by ELISA assay. This study not only increases our background knowledge about and scientific insight into the mechanisms of HFMD, but also reveals novel potential biomarkers for the clinical diagnosis of severe HFMD.http://europepmc.org/articles/PMC4182520?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jianjun Liu Peiwu Huang Yaqing He Wen-Xu Hong Xiaohu Ren Xifei Yang Yanxia He Wenjian Wang Renli Zhang Hong Yang Zhiguang Zhao Haiyan Huang Long Chen Dejian Zhao Huixia Xian Fang Yang Dongli Ma Linqing Yang Yundong Yin Li Zhou Xiaozhen Chen Jinquan Cheng |
spellingShingle |
Jianjun Liu Peiwu Huang Yaqing He Wen-Xu Hong Xiaohu Ren Xifei Yang Yanxia He Wenjian Wang Renli Zhang Hong Yang Zhiguang Zhao Haiyan Huang Long Chen Dejian Zhao Huixia Xian Fang Yang Dongli Ma Linqing Yang Yundong Yin Li Zhou Xiaozhen Chen Jinquan Cheng Serum amyloid A and clusterin as potential predictive biomarkers for severe hand, foot and mouth disease by 2D-DIGE proteomics analysis. PLoS ONE |
author_facet |
Jianjun Liu Peiwu Huang Yaqing He Wen-Xu Hong Xiaohu Ren Xifei Yang Yanxia He Wenjian Wang Renli Zhang Hong Yang Zhiguang Zhao Haiyan Huang Long Chen Dejian Zhao Huixia Xian Fang Yang Dongli Ma Linqing Yang Yundong Yin Li Zhou Xiaozhen Chen Jinquan Cheng |
author_sort |
Jianjun Liu |
title |
Serum amyloid A and clusterin as potential predictive biomarkers for severe hand, foot and mouth disease by 2D-DIGE proteomics analysis. |
title_short |
Serum amyloid A and clusterin as potential predictive biomarkers for severe hand, foot and mouth disease by 2D-DIGE proteomics analysis. |
title_full |
Serum amyloid A and clusterin as potential predictive biomarkers for severe hand, foot and mouth disease by 2D-DIGE proteomics analysis. |
title_fullStr |
Serum amyloid A and clusterin as potential predictive biomarkers for severe hand, foot and mouth disease by 2D-DIGE proteomics analysis. |
title_full_unstemmed |
Serum amyloid A and clusterin as potential predictive biomarkers for severe hand, foot and mouth disease by 2D-DIGE proteomics analysis. |
title_sort |
serum amyloid a and clusterin as potential predictive biomarkers for severe hand, foot and mouth disease by 2d-dige proteomics analysis. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2014-01-01 |
description |
Hand, foot, and mouth disease (HFMD) affects more than one million children, is responsible for several hundred child deaths every year in China and is the cause of widespread concerns in society. Only a small fraction of HFMD cases will develop further into severe HFMD with neurologic complications. A timely and accurate diagnosis of severe HFMD is essential for assessing the risk of progression and planning the appropriate treatment. Human serum can reflect the physiological or pathological states, which is expected to be an excellent source of disease-specific biomarkers. In the present study, a comparative serological proteome analysis between severe HFMD patients and healthy controls was performed via a two-dimensional difference gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) strategy. Fifteen proteins were identified as differentially expressed in the sera of the severe HFMD patients compared with the controls. The identified proteins were classified into different groups according to their molecular functions, biological processes, protein classes and physiological pathways by bioinformatics analysis. The up-regulations of two identified proteins, serum amyloid A (SAA) and clusterin (CLU), were confirmed in the sera of the HFMD patients by ELISA assay. This study not only increases our background knowledge about and scientific insight into the mechanisms of HFMD, but also reveals novel potential biomarkers for the clinical diagnosis of severe HFMD. |
url |
http://europepmc.org/articles/PMC4182520?pdf=render |
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