Comparison of Aflatoxin B1 production by Aspergillus flavus and Aspergillus parasiticus under various conditions of temperature, light and pH

Abstract Background & aim: Aflatoxins are a large group of mycotoxins. The aim of the present study was the comparison of Aflatoxin B1 (AFB1) production by Aspergillus flavus (IR 111) and Aspergillus parasiticus (NRRL 2999) under various conditions of temperature, light, and pH. Methods: In t...

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Main Authors: O Fani makk, N Afzali, A Omidi, A Shibak
Format: Article
Language:fas
Published: Yasuj University Of Medical Sciences 2013-07-01
Series:Armaghane Danesh Bimonthly Journal
Subjects:
Online Access:http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-1-227&slc_lang=en&sid=1
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spelling doaj-ef3ff68c3f624b818715f9bf373bde562020-11-25T00:03:24ZfasYasuj University Of Medical SciencesArmaghane Danesh Bimonthly Journal1728-65061728-65142013-07-01183210218Comparison of Aflatoxin B1 production by Aspergillus flavus and Aspergillus parasiticus under various conditions of temperature, light and pHO Fani makk0N Afzali1A Omidi2A Shibak3 Abstract Background & aim: Aflatoxins are a large group of mycotoxins. The aim of the present study was the comparison of Aflatoxin B1 (AFB1) production by Aspergillus flavus (IR 111) and Aspergillus parasiticus (NRRL 2999) under various conditions of temperature, light, and pH. Methods: In this experimental study, twenty-four flasks were assigned for incubation of each of the fungi A. Flavus and parasiticus at 18, 24, 32 °C. Both flasks were maintained under conditions of light and darkness. The rate of (AFB1) produced by each groups, was measured by thin layer chromatography. Data were analyzed using descriptive statistical analysis (SPSS, version 16). Results: The lowest yield of (AFB1) produced by A. Flavus and parasiticus belonged to 32 °C and pH 6.5, respectively. On the other hand, the highest yield of toxin was observed at 24 °C and pH 6. The lighting effects were considerable. According to the studies on the adverse effects of light and the fermentation process, aflatoxin production increased in dark conditions. Conclusion: The results of study showed that A. Parasiticus (NRRL 2999) produced more aflatoxin than A. Flavus (IR 111). Key words: Aflatoxin B1, Aspergillus flavus, Aspergillus parasiticushttp://armaghanj.yums.ac.ir/browse.php?a_code=A-10-1-227&slc_lang=en&sid=1Key words: Aflatoxin B1 Aspergillus flavus Aspergillus parasiticus
collection DOAJ
language fas
format Article
sources DOAJ
author O Fani makk
N Afzali
A Omidi
A Shibak
spellingShingle O Fani makk
N Afzali
A Omidi
A Shibak
Comparison of Aflatoxin B1 production by Aspergillus flavus and Aspergillus parasiticus under various conditions of temperature, light and pH
Armaghane Danesh Bimonthly Journal
Key words: Aflatoxin B1
Aspergillus flavus
Aspergillus parasiticus
author_facet O Fani makk
N Afzali
A Omidi
A Shibak
author_sort O Fani makk
title Comparison of Aflatoxin B1 production by Aspergillus flavus and Aspergillus parasiticus under various conditions of temperature, light and pH
title_short Comparison of Aflatoxin B1 production by Aspergillus flavus and Aspergillus parasiticus under various conditions of temperature, light and pH
title_full Comparison of Aflatoxin B1 production by Aspergillus flavus and Aspergillus parasiticus under various conditions of temperature, light and pH
title_fullStr Comparison of Aflatoxin B1 production by Aspergillus flavus and Aspergillus parasiticus under various conditions of temperature, light and pH
title_full_unstemmed Comparison of Aflatoxin B1 production by Aspergillus flavus and Aspergillus parasiticus under various conditions of temperature, light and pH
title_sort comparison of aflatoxin b1 production by aspergillus flavus and aspergillus parasiticus under various conditions of temperature, light and ph
publisher Yasuj University Of Medical Sciences
series Armaghane Danesh Bimonthly Journal
issn 1728-6506
1728-6514
publishDate 2013-07-01
description Abstract Background & aim: Aflatoxins are a large group of mycotoxins. The aim of the present study was the comparison of Aflatoxin B1 (AFB1) production by Aspergillus flavus (IR 111) and Aspergillus parasiticus (NRRL 2999) under various conditions of temperature, light, and pH. Methods: In this experimental study, twenty-four flasks were assigned for incubation of each of the fungi A. Flavus and parasiticus at 18, 24, 32 °C. Both flasks were maintained under conditions of light and darkness. The rate of (AFB1) produced by each groups, was measured by thin layer chromatography. Data were analyzed using descriptive statistical analysis (SPSS, version 16). Results: The lowest yield of (AFB1) produced by A. Flavus and parasiticus belonged to 32 °C and pH 6.5, respectively. On the other hand, the highest yield of toxin was observed at 24 °C and pH 6. The lighting effects were considerable. According to the studies on the adverse effects of light and the fermentation process, aflatoxin production increased in dark conditions. Conclusion: The results of study showed that A. Parasiticus (NRRL 2999) produced more aflatoxin than A. Flavus (IR 111). Key words: Aflatoxin B1, Aspergillus flavus, Aspergillus parasiticus
topic Key words: Aflatoxin B1
Aspergillus flavus
Aspergillus parasiticus
url http://armaghanj.yums.ac.ir/browse.php?a_code=A-10-1-227&slc_lang=en&sid=1
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