Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII)
The evaluation of transgenic plants which is usually carried out under controlled conditions in culture rooms and greenhouses can yield valuable information about the influence of introduced genes on a transgenic plant phenotype. However, an overall assessment of plant performance can only...
Main Authors: | , , , , , , |
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Format: | Article |
Language: | English |
Published: |
University of Belgrade, University of Novi Sad
2015-01-01
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Series: | Archives of Biological Sciences |
Subjects: | |
Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641500058C.pdf |
Summary: | The evaluation of transgenic plants which is usually carried out under
controlled conditions in culture rooms and greenhouses can yield valuable
information about the influence of introduced genes on a transgenic plant
phenotype. However, an overall assessment of plant performance can only be
made by testing transgenic plants in the field environment. Thus, the effects
of pyramided rice cystatin genes OCI and OCII on morphological parameters of
transgenic potato cv. Desiree, Dragačevka and Jelica lines were compared
under in vitro, greenhouse, and field conditions. All analyzed OC
co-expressing transgenic lines exhibited normal phenotype, both in vitro and
in greenhouse conditions. In the field environment, eight of nine OCI/OCII
lines were similar to the wild-type control plants in their general
phenotypic appearance. Yield parameters, such as tuber number and tuber
weight for these phenotypically normal OCI/OCII lines, were also comparable
to the controls. Only transgenic cv. Jelica line 4 plants exhibited slightly
reduced growth, atypical leaf morphology and, contrary to the plants of other
transgenic lines and untransformed controls, failed to flower. However,
despite the phenotypic and developmental changes under field conditions, the
OCI/OCII Jelica line 4 did not exhibit a significant decrease in tuber yield.
Stacking of OCI and OCII genes preserves important attributes of the parental
lines, confirming that this approach could be suitable for improving
agronomical traits in potato. [Projekat Ministarstva nauke Republike Srbije,
br. ON173015] |
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ISSN: | 0354-4664 1821-4339 |