Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII)
The evaluation of transgenic plants which is usually carried out under controlled conditions in culture rooms and greenhouses can yield valuable information about the influence of introduced genes on a transgenic plant phenotype. However, an overall assessment of plant performance can only...
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University of Belgrade, University of Novi Sad
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doaj-ef4f289bc88048db9ae24c59550ca8892020-11-24T22:26:27ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46641821-43392015-01-0167395796410.2298/ABS141201058C0354-46641500058CPhenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII)Cingel Aleksandar0Savić Jelena1Ćosić Tatjana2Raspor Martin3Ghalawenji Nabil4Smigocki Ann5Ninković Slavica6Institute for Biological Research “Siniša Stanković”, Department of Plant Physiology, BelgradeInstitute for Biological Research “Siniša Stanković”, Department of Plant Physiology, BelgradeInstitute for Biological Research “Siniša Stanković”, Department of Plant Physiology, BelgradeInstitute for Biological Research “Siniša Stanković”, Department of Plant Physiology, BelgradeInstitute for Biological Research “Siniša Stanković”, Department of Plant Physiology, BelgradeUSDA-ARS, Molecular Plant Pathology Laboratory, Beltsville, USAInstitute for Biological Research “Siniša Stanković”, Department of Plant Physiology, BelgradeThe evaluation of transgenic plants which is usually carried out under controlled conditions in culture rooms and greenhouses can yield valuable information about the influence of introduced genes on a transgenic plant phenotype. However, an overall assessment of plant performance can only be made by testing transgenic plants in the field environment. Thus, the effects of pyramided rice cystatin genes OCI and OCII on morphological parameters of transgenic potato cv. Desiree, Dragačevka and Jelica lines were compared under in vitro, greenhouse, and field conditions. All analyzed OC co-expressing transgenic lines exhibited normal phenotype, both in vitro and in greenhouse conditions. In the field environment, eight of nine OCI/OCII lines were similar to the wild-type control plants in their general phenotypic appearance. Yield parameters, such as tuber number and tuber weight for these phenotypically normal OCI/OCII lines, were also comparable to the controls. Only transgenic cv. Jelica line 4 plants exhibited slightly reduced growth, atypical leaf morphology and, contrary to the plants of other transgenic lines and untransformed controls, failed to flower. However, despite the phenotypic and developmental changes under field conditions, the OCI/OCII Jelica line 4 did not exhibit a significant decrease in tuber yield. Stacking of OCI and OCII genes preserves important attributes of the parental lines, confirming that this approach could be suitable for improving agronomical traits in potato. [Projekat Ministarstva nauke Republike Srbije, br. ON173015]http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641500058C.pdftransgenic potato plantsrice cystatinsOCIOCIIfield performances |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Cingel Aleksandar Savić Jelena Ćosić Tatjana Raspor Martin Ghalawenji Nabil Smigocki Ann Ninković Slavica |
spellingShingle |
Cingel Aleksandar Savić Jelena Ćosić Tatjana Raspor Martin Ghalawenji Nabil Smigocki Ann Ninković Slavica Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII) Archives of Biological Sciences transgenic potato plants rice cystatins OCI OCII field performances |
author_facet |
Cingel Aleksandar Savić Jelena Ćosić Tatjana Raspor Martin Ghalawenji Nabil Smigocki Ann Ninković Slavica |
author_sort |
Cingel Aleksandar |
title |
Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII) |
title_short |
Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII) |
title_full |
Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII) |
title_fullStr |
Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII) |
title_full_unstemmed |
Phenotypic performance of transgenic potato (Solanum tuberosum L.) plants with pyramided rice cystatin genes (OCI and OCII) |
title_sort |
phenotypic performance of transgenic potato (solanum tuberosum l.) plants with pyramided rice cystatin genes (oci and ocii) |
publisher |
University of Belgrade, University of Novi Sad |
series |
Archives of Biological Sciences |
issn |
0354-4664 1821-4339 |
publishDate |
2015-01-01 |
description |
The evaluation of transgenic plants which is usually carried out under
controlled conditions in culture rooms and greenhouses can yield valuable
information about the influence of introduced genes on a transgenic plant
phenotype. However, an overall assessment of plant performance can only be
made by testing transgenic plants in the field environment. Thus, the effects
of pyramided rice cystatin genes OCI and OCII on morphological parameters of
transgenic potato cv. Desiree, Dragačevka and Jelica lines were compared
under in vitro, greenhouse, and field conditions. All analyzed OC
co-expressing transgenic lines exhibited normal phenotype, both in vitro and
in greenhouse conditions. In the field environment, eight of nine OCI/OCII
lines were similar to the wild-type control plants in their general
phenotypic appearance. Yield parameters, such as tuber number and tuber
weight for these phenotypically normal OCI/OCII lines, were also comparable
to the controls. Only transgenic cv. Jelica line 4 plants exhibited slightly
reduced growth, atypical leaf morphology and, contrary to the plants of other
transgenic lines and untransformed controls, failed to flower. However,
despite the phenotypic and developmental changes under field conditions, the
OCI/OCII Jelica line 4 did not exhibit a significant decrease in tuber yield.
Stacking of OCI and OCII genes preserves important attributes of the parental
lines, confirming that this approach could be suitable for improving
agronomical traits in potato. [Projekat Ministarstva nauke Republike Srbije,
br. ON173015] |
topic |
transgenic potato plants rice cystatins OCI OCII field performances |
url |
http://www.doiserbia.nb.rs/img/doi/0354-4664/2015/0354-46641500058C.pdf |
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