The Role of Gold Nanorods in the Response of Prostate Cancer and Normal Prostate Cells to Ionizing Radiation—In Vitro Model
To increase the efficiency of therapy via enhancing its selectivity, the usage of gold nanorods (GNR) as a factor sensitizing cancer cells to radiation was proposed. Due to gold nanoparticles’ characteristics, the smaller doses of radiation would be sufficient in the treatment, protecting the health...
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doaj-ef5c5f030ccd4005913f2607a064bb7f2020-12-23T00:01:30ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-12-0122161610.3390/ijms22010016The Role of Gold Nanorods in the Response of Prostate Cancer and Normal Prostate Cells to Ionizing Radiation—In Vitro ModelMarika Musielak0Agnieszka Boś-Liedke1Igor Piotrowski2Maciej Kozak3Wiktoria Suchorska4Radiobiology Laboratory, Department of Medical Physics, Greater Poland Cancer Centre, 61-866 Poznań, PolandDepartment of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University, 61-614 Poznań, PolandRadiobiology Laboratory, Department of Medical Physics, Greater Poland Cancer Centre, 61-866 Poznań, PolandDepartment of Macromolecular Physics, Faculty of Physics, Adam Mickiewicz University, 61-614 Poznań, PolandRadiobiology Laboratory, Department of Medical Physics, Greater Poland Cancer Centre, 61-866 Poznań, PolandTo increase the efficiency of therapy via enhancing its selectivity, the usage of gold nanorods (GNR) as a factor sensitizing cancer cells to radiation was proposed. Due to gold nanoparticles’ characteristics, the smaller doses of radiation would be sufficient in the treatment, protecting the healthy tissue around the tumor. The aim of this study was to investigate the effect of gold nanorods on cancer and normal prostate cells and the role of nanorods in the cell response to ionizing radiation. The effect was evaluated by measuring the toxicity, cell cycle, cell granularity, reactive oxygen species (ROS) level, and survival fractions. Nanorods showed a strong toxicity dependent on the concentration and incubation time toward all used cell lines. A slight effect of nanorods on the cycle distribution was observed. The results demonstrated that the administration of nanorods at higher concentrations resulted in an increased level of generated radicals. The results of cellular proliferation after irradiation are ambiguous; however, there are noticeable differences after the application of nanorods before irradiation. The obtained results lead to the conclusion that nanorods affect the physiology of both normal and cancer cells. Nanorods might become a potential tool used to increase the effectiveness of radiation treatmenthttps://www.mdpi.com/1422-0067/22/1/16gold nanorodsnanoparticlesradiotherapyprostate cancerradiosensitivity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Marika Musielak Agnieszka Boś-Liedke Igor Piotrowski Maciej Kozak Wiktoria Suchorska |
spellingShingle |
Marika Musielak Agnieszka Boś-Liedke Igor Piotrowski Maciej Kozak Wiktoria Suchorska The Role of Gold Nanorods in the Response of Prostate Cancer and Normal Prostate Cells to Ionizing Radiation—In Vitro Model International Journal of Molecular Sciences gold nanorods nanoparticles radiotherapy prostate cancer radiosensitivity |
author_facet |
Marika Musielak Agnieszka Boś-Liedke Igor Piotrowski Maciej Kozak Wiktoria Suchorska |
author_sort |
Marika Musielak |
title |
The Role of Gold Nanorods in the Response of Prostate Cancer and Normal Prostate Cells to Ionizing Radiation—In Vitro Model |
title_short |
The Role of Gold Nanorods in the Response of Prostate Cancer and Normal Prostate Cells to Ionizing Radiation—In Vitro Model |
title_full |
The Role of Gold Nanorods in the Response of Prostate Cancer and Normal Prostate Cells to Ionizing Radiation—In Vitro Model |
title_fullStr |
The Role of Gold Nanorods in the Response of Prostate Cancer and Normal Prostate Cells to Ionizing Radiation—In Vitro Model |
title_full_unstemmed |
The Role of Gold Nanorods in the Response of Prostate Cancer and Normal Prostate Cells to Ionizing Radiation—In Vitro Model |
title_sort |
role of gold nanorods in the response of prostate cancer and normal prostate cells to ionizing radiation—in vitro model |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-12-01 |
description |
To increase the efficiency of therapy via enhancing its selectivity, the usage of gold nanorods (GNR) as a factor sensitizing cancer cells to radiation was proposed. Due to gold nanoparticles’ characteristics, the smaller doses of radiation would be sufficient in the treatment, protecting the healthy tissue around the tumor. The aim of this study was to investigate the effect of gold nanorods on cancer and normal prostate cells and the role of nanorods in the cell response to ionizing radiation. The effect was evaluated by measuring the toxicity, cell cycle, cell granularity, reactive oxygen species (ROS) level, and survival fractions. Nanorods showed a strong toxicity dependent on the concentration and incubation time toward all used cell lines. A slight effect of nanorods on the cycle distribution was observed. The results demonstrated that the administration of nanorods at higher concentrations resulted in an increased level of generated radicals. The results of cellular proliferation after irradiation are ambiguous; however, there are noticeable differences after the application of nanorods before irradiation. The obtained results lead to the conclusion that nanorods affect the physiology of both normal and cancer cells. Nanorods might become a potential tool used to increase the effectiveness of radiation treatment |
topic |
gold nanorods nanoparticles radiotherapy prostate cancer radiosensitivity |
url |
https://www.mdpi.com/1422-0067/22/1/16 |
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