Host-associated microbe PCR (hamPCR) enables convenient measurement of both microbial load and community composition

Host-associated microbe PCR (hamPCR) enables convenient measurement of both microbial load and community composition

The ratio of microbial population size relative to the amount of host tissue, or ‘microbial load’, is a fundamental metric of colonization and infection, but it cannot be directly deduced from microbial amplicon data such as 16S rRNA gene counts. Because existing methods to determine load, such as s...

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Bibliographic Details
Main Authors: Derek S Lundberg, Pratchaya Pramoj Na Ayutthaya, Annett Strauß, Gautam Shirsekar, Wen-Sui Lo, Thomas Lahaye, Detlef Weigel
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2021-07-01
Series:eLife
Subjects:
amplicon sequencing
microbiome
absolute abundance
nematode
crop plants
quantitative
Online Access:https://elifesciences.org/articles/66186

Internet

https://elifesciences.org/articles/66186

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