In Vitro Effect of Ananas Comosus Extract on Human Sperm
Spermicides has been used for birth control, but its chemical components are harmful for the vaginal epithelium, thus other studies have been done, given the possibility to use different extracts from plants as spermicidal agents that avoid vaginal tissue damages. The aim of this study was to evalua...
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Instituto Tecnológico Metropolitano
2012-06-01
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Online Access: | http://itmojs.itm.edu.co/index.php/tecnologicas/article/view/204/210 |
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doaj-ef6c370206fb409697a06939bf40cad22020-11-24T21:15:53ZengInstituto Tecnológico MetropolitanoTecnoLógicas0123-77992256-53372012-06-01285570In Vitro Effect of Ananas Comosus Extract on Human SpermMélany Uribe-ClavijoÁngela M. Álvarez-GómezVíctor ArangoFabian M. Cortes-ManceraÁngela P. Cadavid-JaramilloWalter D. Cardona-MayaSpermicides has been used for birth control, but its chemical components are harmful for the vaginal epithelium, thus other studies have been done, given the possibility to use different extracts from plants as spermicidal agents that avoid vaginal tissue damages. The aim of this study was to evaluate the Ananas comosus extract as spermicidal. Semen samples were incubated with pineapple extract, and motility and viability was evaluated after contact. In addition the cytotoxic effect was evaluated using MTS assay on HeLa cells. Motility (p<0.001) and viability (p<0.001) decreased after treatment with 50% dilution. However, extract had a cytotoxic effect on epithelial cell line. In conclusion, despite the cytotoxic effect in the same concentrations that spermicidal activity was observed, further studies determining the fractions of the extract have spermicidal properties are necessary.http://itmojs.itm.edu.co/index.php/tecnologicas/article/view/204/210SpermatozoaspermicidesAnanas comosuscontraceptionhyperactivation. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mélany Uribe-Clavijo Ángela M. Álvarez-Gómez Víctor Arango Fabian M. Cortes-Mancera Ángela P. Cadavid-Jaramillo Walter D. Cardona-Maya |
spellingShingle |
Mélany Uribe-Clavijo Ángela M. Álvarez-Gómez Víctor Arango Fabian M. Cortes-Mancera Ángela P. Cadavid-Jaramillo Walter D. Cardona-Maya In Vitro Effect of Ananas Comosus Extract on Human Sperm TecnoLógicas Spermatozoa spermicides Ananas comosus contraception hyperactivation. |
author_facet |
Mélany Uribe-Clavijo Ángela M. Álvarez-Gómez Víctor Arango Fabian M. Cortes-Mancera Ángela P. Cadavid-Jaramillo Walter D. Cardona-Maya |
author_sort |
Mélany Uribe-Clavijo |
title |
In Vitro Effect of Ananas Comosus Extract on Human Sperm |
title_short |
In Vitro Effect of Ananas Comosus Extract on Human Sperm |
title_full |
In Vitro Effect of Ananas Comosus Extract on Human Sperm |
title_fullStr |
In Vitro Effect of Ananas Comosus Extract on Human Sperm |
title_full_unstemmed |
In Vitro Effect of Ananas Comosus Extract on Human Sperm |
title_sort |
in vitro effect of ananas comosus extract on human sperm |
publisher |
Instituto Tecnológico Metropolitano |
series |
TecnoLógicas |
issn |
0123-7799 2256-5337 |
publishDate |
2012-06-01 |
description |
Spermicides has been used for birth control, but its chemical components are harmful for the vaginal epithelium, thus other studies have been done, given the possibility to use different extracts from plants as spermicidal agents that avoid vaginal tissue damages. The aim of this study was to evaluate the Ananas comosus extract as spermicidal. Semen samples were incubated with pineapple extract, and motility and viability was evaluated after contact. In addition the cytotoxic effect was evaluated using MTS assay on HeLa cells. Motility (p<0.001) and viability (p<0.001) decreased after treatment with 50% dilution. However, extract had a cytotoxic effect on epithelial cell line. In conclusion, despite the cytotoxic effect in the same concentrations that spermicidal activity was observed, further studies determining the fractions of the extract have spermicidal properties are necessary. |
topic |
Spermatozoa spermicides Ananas comosus contraception hyperactivation. |
url |
http://itmojs.itm.edu.co/index.php/tecnologicas/article/view/204/210 |
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