Introgression of Maize Lethal Necrosis Resistance Quantitative Trait Loci Into Susceptible Maize Populations and Validation of the Resistance Under Field Conditions in Naivasha, Kenya
Maize lethal necrosis (MLN), resulting from co-infection by maize chlorotic mottle virus (MCMV) and sugarcane mosaic virus (SCMV) can cause up to 100% yield losses in maize in Africa under serious disease conditions. Maize improvement through conventional backcross (BC) takes many generations but ca...
Main Authors: | , , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Frontiers Media S.A.
2021-05-01
|
Series: | Frontiers in Plant Science |
Subjects: | |
Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.649308/full |
id |
doaj-ef73a2ea40194447b440b055477ed075 |
---|---|
record_format |
Article |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Luka A. O. Awata Beatrice E. Ifie Eric Danquah MacDonald Bright Jumbo L. Mahabaleswara Suresh Manje Gowda Philip W. Marchelo-Dragga Michael Scott Olsen Oluwaseyi Shorinola Oluwaseyi Shorinola Nasser Kouadio Yao Prasanna M. Boddupalli Pangirayi B. Tongoona |
spellingShingle |
Luka A. O. Awata Beatrice E. Ifie Eric Danquah MacDonald Bright Jumbo L. Mahabaleswara Suresh Manje Gowda Philip W. Marchelo-Dragga Michael Scott Olsen Oluwaseyi Shorinola Oluwaseyi Shorinola Nasser Kouadio Yao Prasanna M. Boddupalli Pangirayi B. Tongoona Introgression of Maize Lethal Necrosis Resistance Quantitative Trait Loci Into Susceptible Maize Populations and Validation of the Resistance Under Field Conditions in Naivasha, Kenya Frontiers in Plant Science maize backcross kompetitive allele specific PCR alleles maize lethal necrosis introgression |
author_facet |
Luka A. O. Awata Beatrice E. Ifie Eric Danquah MacDonald Bright Jumbo L. Mahabaleswara Suresh Manje Gowda Philip W. Marchelo-Dragga Michael Scott Olsen Oluwaseyi Shorinola Oluwaseyi Shorinola Nasser Kouadio Yao Prasanna M. Boddupalli Pangirayi B. Tongoona |
author_sort |
Luka A. O. Awata |
title |
Introgression of Maize Lethal Necrosis Resistance Quantitative Trait Loci Into Susceptible Maize Populations and Validation of the Resistance Under Field Conditions in Naivasha, Kenya |
title_short |
Introgression of Maize Lethal Necrosis Resistance Quantitative Trait Loci Into Susceptible Maize Populations and Validation of the Resistance Under Field Conditions in Naivasha, Kenya |
title_full |
Introgression of Maize Lethal Necrosis Resistance Quantitative Trait Loci Into Susceptible Maize Populations and Validation of the Resistance Under Field Conditions in Naivasha, Kenya |
title_fullStr |
Introgression of Maize Lethal Necrosis Resistance Quantitative Trait Loci Into Susceptible Maize Populations and Validation of the Resistance Under Field Conditions in Naivasha, Kenya |
title_full_unstemmed |
Introgression of Maize Lethal Necrosis Resistance Quantitative Trait Loci Into Susceptible Maize Populations and Validation of the Resistance Under Field Conditions in Naivasha, Kenya |
title_sort |
introgression of maize lethal necrosis resistance quantitative trait loci into susceptible maize populations and validation of the resistance under field conditions in naivasha, kenya |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Plant Science |
issn |
1664-462X |
publishDate |
2021-05-01 |
description |
Maize lethal necrosis (MLN), resulting from co-infection by maize chlorotic mottle virus (MCMV) and sugarcane mosaic virus (SCMV) can cause up to 100% yield losses in maize in Africa under serious disease conditions. Maize improvement through conventional backcross (BC) takes many generations but can significantly be shortened when molecular tools are utilized in the breeding process. We used a donor parent (KS23-6) to transfer quantitative trait loci (QTL) for resistance to MLN into nine adapted but MLN susceptible lines. Nurseries were established in Kiboko, Kenya during 2015–2017 seasons and BC3F2 progeny were developed using marker assisted backcrossing (MABC) approach. Six single nucleotide polymorphism (SNP) markers linked to QTL for resistance to MLN were used to genotype 2,400 BC3F2 lines using Kompetitive Allele Specific PCR (KASP) platform. We detected that two of the six QTL had major effects for resistance to MLN under artificial inoculation field conditions in 56 candidate BC3F2 lines. To confirm whether these two QTL are reproducible under different field conditions, the 56 BC3F2 lines including their parents were evaluated in replicated trials for two seasons under artificial MLN inoculations in Naivasha, Kenya in 2018. Strong association of genotype with phenotype was detected. Consequently, 19 superior BC3F2 lines with favorable alleles and showing improved levels of resistance to MLN under artificial field inoculation were identified. These elite lines represent superior genetic resources for improvement of maize hybrids for resistance to MLN. However, 20 BC3F2 lines were fixed for both KASP markers but were susceptible to MLN under field conditions, which could suggest weak linkage between the KASP markers and target genes. The validated two major QTL can be utilized to speed up the breeding process but additional loci need to be identified between the KASP markers and the resistance genes to strengthen the linkage. |
topic |
maize backcross kompetitive allele specific PCR alleles maize lethal necrosis introgression |
url |
https://www.frontiersin.org/articles/10.3389/fpls.2021.649308/full |
work_keys_str_mv |
AT lukaaoawata introgressionofmaizelethalnecrosisresistancequantitativetraitlociintosusceptiblemaizepopulationsandvalidationoftheresistanceunderfieldconditionsinnaivashakenya AT beatriceeifie introgressionofmaizelethalnecrosisresistancequantitativetraitlociintosusceptiblemaizepopulationsandvalidationoftheresistanceunderfieldconditionsinnaivashakenya AT ericdanquah introgressionofmaizelethalnecrosisresistancequantitativetraitlociintosusceptiblemaizepopulationsandvalidationoftheresistanceunderfieldconditionsinnaivashakenya AT macdonaldbrightjumbo introgressionofmaizelethalnecrosisresistancequantitativetraitlociintosusceptiblemaizepopulationsandvalidationoftheresistanceunderfieldconditionsinnaivashakenya AT lmahabaleswarasuresh introgressionofmaizelethalnecrosisresistancequantitativetraitlociintosusceptiblemaizepopulationsandvalidationoftheresistanceunderfieldconditionsinnaivashakenya AT manjegowda introgressionofmaizelethalnecrosisresistancequantitativetraitlociintosusceptiblemaizepopulationsandvalidationoftheresistanceunderfieldconditionsinnaivashakenya AT philipwmarchelodragga introgressionofmaizelethalnecrosisresistancequantitativetraitlociintosusceptiblemaizepopulationsandvalidationoftheresistanceunderfieldconditionsinnaivashakenya AT michaelscottolsen introgressionofmaizelethalnecrosisresistancequantitativetraitlociintosusceptiblemaizepopulationsandvalidationoftheresistanceunderfieldconditionsinnaivashakenya AT oluwaseyishorinola introgressionofmaizelethalnecrosisresistancequantitativetraitlociintosusceptiblemaizepopulationsandvalidationoftheresistanceunderfieldconditionsinnaivashakenya AT oluwaseyishorinola introgressionofmaizelethalnecrosisresistancequantitativetraitlociintosusceptiblemaizepopulationsandvalidationoftheresistanceunderfieldconditionsinnaivashakenya AT nasserkouadioyao introgressionofmaizelethalnecrosisresistancequantitativetraitlociintosusceptiblemaizepopulationsandvalidationoftheresistanceunderfieldconditionsinnaivashakenya AT prasannamboddupalli introgressionofmaizelethalnecrosisresistancequantitativetraitlociintosusceptiblemaizepopulationsandvalidationoftheresistanceunderfieldconditionsinnaivashakenya AT pangirayibtongoona introgressionofmaizelethalnecrosisresistancequantitativetraitlociintosusceptiblemaizepopulationsandvalidationoftheresistanceunderfieldconditionsinnaivashakenya |
_version_ |
1721453207192862720 |
spelling |
doaj-ef73a2ea40194447b440b055477ed0752021-05-10T16:07:17ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-05-011210.3389/fpls.2021.649308649308Introgression of Maize Lethal Necrosis Resistance Quantitative Trait Loci Into Susceptible Maize Populations and Validation of the Resistance Under Field Conditions in Naivasha, KenyaLuka A. O. Awata0Beatrice E. Ifie1Eric Danquah2MacDonald Bright Jumbo3L. Mahabaleswara Suresh4Manje Gowda5Philip W. Marchelo-Dragga6Michael Scott Olsen7Oluwaseyi Shorinola8Oluwaseyi Shorinola9Nasser Kouadio Yao10Prasanna M. Boddupalli11Pangirayi B. Tongoona12Directorate of Research, Ministry of Agriculture and Food Security, Juba, South SudanWest Africa Centre for Crop Improvement (WACCI), College of Basic and Applied Sciences, University of Ghana, Legon, GhanaWest Africa Centre for Crop Improvement (WACCI), College of Basic and Applied Sciences, University of Ghana, Legon, GhanaInternational Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Bulawayo, ZimbabweInternational Maize and Wheat Improvement Center (CIMMYT), World Agroforestry Centre (ICRAF), Nairobi, KenyaInternational Maize and Wheat Improvement Center (CIMMYT), World Agroforestry Centre (ICRAF), Nairobi, KenyaDepartment of Agricultural Sciences, College of Natural Resources and Environmental Studies, University of Juba, Juba, South SudanInternational Maize and Wheat Improvement Center (CIMMYT), World Agroforestry Centre (ICRAF), Nairobi, KenyaBiosciences eastern and central Africa (BecA) Hub, International Livestock Research Institute (ILRI), Nairobi, KenyaJohn Innes Centre, Norwich, United KingdomBiosciences eastern and central Africa (BecA) Hub, International Livestock Research Institute (ILRI), Nairobi, KenyaInternational Maize and Wheat Improvement Center (CIMMYT), World Agroforestry Centre (ICRAF), Nairobi, KenyaWest Africa Centre for Crop Improvement (WACCI), College of Basic and Applied Sciences, University of Ghana, Legon, GhanaMaize lethal necrosis (MLN), resulting from co-infection by maize chlorotic mottle virus (MCMV) and sugarcane mosaic virus (SCMV) can cause up to 100% yield losses in maize in Africa under serious disease conditions. Maize improvement through conventional backcross (BC) takes many generations but can significantly be shortened when molecular tools are utilized in the breeding process. We used a donor parent (KS23-6) to transfer quantitative trait loci (QTL) for resistance to MLN into nine adapted but MLN susceptible lines. Nurseries were established in Kiboko, Kenya during 2015–2017 seasons and BC3F2 progeny were developed using marker assisted backcrossing (MABC) approach. Six single nucleotide polymorphism (SNP) markers linked to QTL for resistance to MLN were used to genotype 2,400 BC3F2 lines using Kompetitive Allele Specific PCR (KASP) platform. We detected that two of the six QTL had major effects for resistance to MLN under artificial inoculation field conditions in 56 candidate BC3F2 lines. To confirm whether these two QTL are reproducible under different field conditions, the 56 BC3F2 lines including their parents were evaluated in replicated trials for two seasons under artificial MLN inoculations in Naivasha, Kenya in 2018. Strong association of genotype with phenotype was detected. Consequently, 19 superior BC3F2 lines with favorable alleles and showing improved levels of resistance to MLN under artificial field inoculation were identified. These elite lines represent superior genetic resources for improvement of maize hybrids for resistance to MLN. However, 20 BC3F2 lines were fixed for both KASP markers but were susceptible to MLN under field conditions, which could suggest weak linkage between the KASP markers and target genes. The validated two major QTL can be utilized to speed up the breeding process but additional loci need to be identified between the KASP markers and the resistance genes to strengthen the linkage.https://www.frontiersin.org/articles/10.3389/fpls.2021.649308/fullmaizebackcrosskompetitive allele specific PCRallelesmaize lethal necrosisintrogression |