Novel CYP1B1 mutations and a possible prognostic use for surgical management of congenital glaucoma
AIM: To identify CYP1B1 gene mutations and evaluate their possible role as a prognostic factor for success rates in the surgical management of Egyptian congenital glaucoma patients. METHODS: Totally 42 eyes of 29 primary congenital glaucoma patients were operated on with combined trabeculotomy/trab...
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doaj-ef798cab67e6444186212fc3e2e0b7792020-11-25T00:56:31ZengPress of International Journal of Ophthalmology (IJO PRESS)International Journal of Ophthalmology2222-39592227-48982019-04-0112460761410.18240/ijo.2019.04.14Novel CYP1B1 mutations and a possible prognostic use for surgical management of congenital glaucomaMohamed M. Khafagy0Nadia El-Guendy1Marwa A Tantawy2Mohamed A. Eldaly3Hala M. Elhilali4Abdel Hady A. Abdel Wahab5Ophthalmology Department, Faculty of Medicine, Cairo University, Cairo 11562, EgyptCancer Biology Department, National Cancer Institute, Cairo University, Cairo 11796, EgyptResearch Department, Children’s Cancer Hospital, Cairo 11617, EgyptOphthalmology Department, Faculty of Medicine, Cairo University, Cairo 11562, EgyptOphthalmology Department, Faculty of Medicine, Cairo University, Cairo 11562, EgyptCancer Biology Department, National Cancer Institute, Cairo University, Cairo 11796, EgyptAIM: To identify CYP1B1 gene mutations and evaluate their possible role as a prognostic factor for success rates in the surgical management of Egyptian congenital glaucoma patients. METHODS: Totally 42 eyes of 29 primary congenital glaucoma patients were operated on with combined trabeculotomy/trabeculectomy with mitomycin-C and followed up at 1d, 1wk, 1, 6 and 12mo postoperatively. Genomic DNA was extracted from peripheral blood leukocytes. Coding regions of CYP1B1 gene were amplified using 13 pairs of primers, screened for mutations using single-strand conformation polymorphism followed by sequencing of both strands. Efficacy of the operation was graded as either a success [maintaining intraocular pressure (IOP) less than 21 mm Hg with or without anti-glaucoma medication], or a failure (IOP more than 21 mm Hg with topical antiglaucoma medications). RESULTS: Seven novel mutations out of a total of 15 different mutations were found in the CYP1B1 genes of 14 patients (48.2%). The presence of CYP1B1 gene mutations did not correlate with the failure of the surgery (P=0.156, odds ratio=3.611, 95%CI, 0.56 to 22.89); while the positive consanguinity strongly correlated with failure of the initial procedure (P=0.016, odds ratio=11.25, 95%CI, 1.57 to 80.30). However, the Kaplan-Meier survival analysis revealed a significantly lower time of IOP control in the subgroup with mutations in CYP1B1 versus the congenital primary glaucoma group without mutations (log rank test, P=0.015). CONCLUSION: Seven new CYP1B1 mutations are identified in Egyptian patients. Patients harboring confirmed mutations suffered from early failure of the initial surgery. CYP1B1 mutations could be considered as a prognostic factor for surgery in primary congenital glaucoma.http://www.ijo.cn/en_publish/2019/4/20190414.pdfcyp1b1 mutationsprimary congenital glaucomaintraocular pressuretrabeculotomy/trabeculectomy with mitomycin-c. |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mohamed M. Khafagy Nadia El-Guendy Marwa A Tantawy Mohamed A. Eldaly Hala M. Elhilali Abdel Hady A. Abdel Wahab |
spellingShingle |
Mohamed M. Khafagy Nadia El-Guendy Marwa A Tantawy Mohamed A. Eldaly Hala M. Elhilali Abdel Hady A. Abdel Wahab Novel CYP1B1 mutations and a possible prognostic use for surgical management of congenital glaucoma International Journal of Ophthalmology cyp1b1 mutations primary congenital glaucoma intraocular pressure trabeculotomy/trabeculectomy with mitomycin-c. |
author_facet |
Mohamed M. Khafagy Nadia El-Guendy Marwa A Tantawy Mohamed A. Eldaly Hala M. Elhilali Abdel Hady A. Abdel Wahab |
author_sort |
Mohamed M. Khafagy |
title |
Novel CYP1B1 mutations and a possible prognostic use for surgical management of congenital glaucoma |
title_short |
Novel CYP1B1 mutations and a possible prognostic use for surgical management of congenital glaucoma |
title_full |
Novel CYP1B1 mutations and a possible prognostic use for surgical management of congenital glaucoma |
title_fullStr |
Novel CYP1B1 mutations and a possible prognostic use for surgical management of congenital glaucoma |
title_full_unstemmed |
Novel CYP1B1 mutations and a possible prognostic use for surgical management of congenital glaucoma |
title_sort |
novel cyp1b1 mutations and a possible prognostic use for surgical management of congenital glaucoma |
publisher |
Press of International Journal of Ophthalmology (IJO PRESS) |
series |
International Journal of Ophthalmology |
issn |
2222-3959 2227-4898 |
publishDate |
2019-04-01 |
description |
AIM: To identify CYP1B1 gene mutations and evaluate their possible role as a prognostic factor for success rates in the surgical management of Egyptian congenital glaucoma patients.
METHODS: Totally 42 eyes of 29 primary congenital glaucoma patients were operated on with combined trabeculotomy/trabeculectomy with mitomycin-C and followed up at 1d, 1wk, 1, 6 and 12mo postoperatively. Genomic DNA was extracted from peripheral blood leukocytes. Coding regions of CYP1B1 gene were amplified using 13 pairs of primers, screened for mutations using single-strand conformation polymorphism followed by sequencing of both strands. Efficacy of the operation was graded as either a success [maintaining intraocular pressure (IOP) less than 21 mm Hg with or without anti-glaucoma medication], or a failure (IOP more than 21 mm Hg with topical antiglaucoma medications).
RESULTS: Seven novel mutations out of a total of 15 different mutations were found in the CYP1B1 genes of 14 patients (48.2%). The presence of CYP1B1 gene mutations did not correlate with the failure of the surgery (P=0.156, odds ratio=3.611, 95%CI, 0.56 to 22.89); while the positive consanguinity strongly correlated with failure of the initial procedure (P=0.016, odds ratio=11.25, 95%CI, 1.57 to 80.30). However, the Kaplan-Meier survival analysis revealed a significantly lower time of IOP control in the subgroup with mutations in CYP1B1 versus the congenital primary glaucoma group without mutations (log rank test, P=0.015).
CONCLUSION: Seven new CYP1B1 mutations are identified in Egyptian patients. Patients harboring confirmed mutations suffered from early failure of the initial surgery. CYP1B1 mutations could be considered as a prognostic factor for surgery in primary congenital glaucoma. |
topic |
cyp1b1 mutations primary congenital glaucoma intraocular pressure trabeculotomy/trabeculectomy with mitomycin-c. |
url |
http://www.ijo.cn/en_publish/2019/4/20190414.pdf |
work_keys_str_mv |
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