Molecular Genetic Analysis of Drought Stress Response Traits in <i>Brachypodium</i> spp.

The root is the organ responsible for the uptake of water and therefore has a very important role in drought tolerance. The aims of the present work were to characterize nine traits of the root system architecture (RSA) and the shoot dry weight (W) of twelve genotypes of <i>Brachypodium</i&...

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Main Authors: Juan M. González, Jaime Redondo-Pedraza, Yolanda Loarce, Rifka Hammami, Eva Friero, Nicolás Jouve
Format: Article
Language:English
Published: MDPI AG 2020-04-01
Series:Agronomy
Subjects:
Online Access:https://www.mdpi.com/2073-4395/10/4/518
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spelling doaj-ef83d5ec904840e9b0a0747fb4d579de2021-04-02T14:45:34ZengMDPI AGAgronomy2073-43952020-04-011051851810.3390/agronomy10040518Molecular Genetic Analysis of Drought Stress Response Traits in <i>Brachypodium</i> spp.Juan M. González0Jaime Redondo-Pedraza1Yolanda Loarce2Rifka Hammami3Eva Friero4Nicolás Jouve5Department of Biomedicine and Biotechnology, University of Alcalá, 28805 Alcalá de Henares, Madrid, SpainDepartment of Biomedicine and Biotechnology, University of Alcalá, 28805 Alcalá de Henares, Madrid, SpainDepartment of Biomedicine and Biotechnology, University of Alcalá, 28805 Alcalá de Henares, Madrid, SpainInstitut National de la Recherche Agronomique de Tunis, Rue Hédi Karray 2049, Ariana, TunisiaDepartment of Biomedicine and Biotechnology, University of Alcalá, 28805 Alcalá de Henares, Madrid, SpainDepartment of Biomedicine and Biotechnology, University of Alcalá, 28805 Alcalá de Henares, Madrid, SpainThe root is the organ responsible for the uptake of water and therefore has a very important role in drought tolerance. The aims of the present work were to characterize nine traits of the root system architecture (RSA) and the shoot dry weight (W) of twelve genotypes of <i>Brachypodium</i> spp. under water stress and to establish the relationship between RSA phenotyping traits and SSRs. Two culture media, one standard (SM) and one (PEG) to induced water stress have been used. In SM medium, <i>B. stacei</i> had the highest values of W and all the RSA traits, except the mean diameter of the seminal roots, followed by <i>B. hybridum</i> and <i>B. distachyon</i>. In the PEG medium, root length increased in <i>B. distachyon</i>, decreased in <i>B. hybridum</i> and remained the same in <i>B. stacei</i>. A two-way hierarchical cluster analysis from 117 polymorphic SSRs and the traits of the RSA of the <i>Brachypodium</i> spp. genotypes, was performed. <i>Brachypodium</i> genotypes were separated into three groups corresponding to each species. In the second way of the hierarchical clustering association were observed between five RSA variables and SSR markers, which could be useful in the search for genes or QTLs related to RSA characters.https://www.mdpi.com/2073-4395/10/4/518abiotic stress<i>distachyon</i>cereals<i>hybridum</i>molecular markersroot diversity
collection DOAJ
language English
format Article
sources DOAJ
author Juan M. González
Jaime Redondo-Pedraza
Yolanda Loarce
Rifka Hammami
Eva Friero
Nicolás Jouve
spellingShingle Juan M. González
Jaime Redondo-Pedraza
Yolanda Loarce
Rifka Hammami
Eva Friero
Nicolás Jouve
Molecular Genetic Analysis of Drought Stress Response Traits in <i>Brachypodium</i> spp.
Agronomy
abiotic stress
<i>distachyon</i>
cereals
<i>hybridum</i>
molecular markers
root diversity
author_facet Juan M. González
Jaime Redondo-Pedraza
Yolanda Loarce
Rifka Hammami
Eva Friero
Nicolás Jouve
author_sort Juan M. González
title Molecular Genetic Analysis of Drought Stress Response Traits in <i>Brachypodium</i> spp.
title_short Molecular Genetic Analysis of Drought Stress Response Traits in <i>Brachypodium</i> spp.
title_full Molecular Genetic Analysis of Drought Stress Response Traits in <i>Brachypodium</i> spp.
title_fullStr Molecular Genetic Analysis of Drought Stress Response Traits in <i>Brachypodium</i> spp.
title_full_unstemmed Molecular Genetic Analysis of Drought Stress Response Traits in <i>Brachypodium</i> spp.
title_sort molecular genetic analysis of drought stress response traits in <i>brachypodium</i> spp.
publisher MDPI AG
series Agronomy
issn 2073-4395
publishDate 2020-04-01
description The root is the organ responsible for the uptake of water and therefore has a very important role in drought tolerance. The aims of the present work were to characterize nine traits of the root system architecture (RSA) and the shoot dry weight (W) of twelve genotypes of <i>Brachypodium</i> spp. under water stress and to establish the relationship between RSA phenotyping traits and SSRs. Two culture media, one standard (SM) and one (PEG) to induced water stress have been used. In SM medium, <i>B. stacei</i> had the highest values of W and all the RSA traits, except the mean diameter of the seminal roots, followed by <i>B. hybridum</i> and <i>B. distachyon</i>. In the PEG medium, root length increased in <i>B. distachyon</i>, decreased in <i>B. hybridum</i> and remained the same in <i>B. stacei</i>. A two-way hierarchical cluster analysis from 117 polymorphic SSRs and the traits of the RSA of the <i>Brachypodium</i> spp. genotypes, was performed. <i>Brachypodium</i> genotypes were separated into three groups corresponding to each species. In the second way of the hierarchical clustering association were observed between five RSA variables and SSR markers, which could be useful in the search for genes or QTLs related to RSA characters.
topic abiotic stress
<i>distachyon</i>
cereals
<i>hybridum</i>
molecular markers
root diversity
url https://www.mdpi.com/2073-4395/10/4/518
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