Molecular Genetic Analysis of Drought Stress Response Traits in <i>Brachypodium</i> spp.
The root is the organ responsible for the uptake of water and therefore has a very important role in drought tolerance. The aims of the present work were to characterize nine traits of the root system architecture (RSA) and the shoot dry weight (W) of twelve genotypes of <i>Brachypodium</i&...
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doaj-ef83d5ec904840e9b0a0747fb4d579de2021-04-02T14:45:34ZengMDPI AGAgronomy2073-43952020-04-011051851810.3390/agronomy10040518Molecular Genetic Analysis of Drought Stress Response Traits in <i>Brachypodium</i> spp.Juan M. González0Jaime Redondo-Pedraza1Yolanda Loarce2Rifka Hammami3Eva Friero4Nicolás Jouve5Department of Biomedicine and Biotechnology, University of Alcalá, 28805 Alcalá de Henares, Madrid, SpainDepartment of Biomedicine and Biotechnology, University of Alcalá, 28805 Alcalá de Henares, Madrid, SpainDepartment of Biomedicine and Biotechnology, University of Alcalá, 28805 Alcalá de Henares, Madrid, SpainInstitut National de la Recherche Agronomique de Tunis, Rue Hédi Karray 2049, Ariana, TunisiaDepartment of Biomedicine and Biotechnology, University of Alcalá, 28805 Alcalá de Henares, Madrid, SpainDepartment of Biomedicine and Biotechnology, University of Alcalá, 28805 Alcalá de Henares, Madrid, SpainThe root is the organ responsible for the uptake of water and therefore has a very important role in drought tolerance. The aims of the present work were to characterize nine traits of the root system architecture (RSA) and the shoot dry weight (W) of twelve genotypes of <i>Brachypodium</i> spp. under water stress and to establish the relationship between RSA phenotyping traits and SSRs. Two culture media, one standard (SM) and one (PEG) to induced water stress have been used. In SM medium, <i>B. stacei</i> had the highest values of W and all the RSA traits, except the mean diameter of the seminal roots, followed by <i>B. hybridum</i> and <i>B. distachyon</i>. In the PEG medium, root length increased in <i>B. distachyon</i>, decreased in <i>B. hybridum</i> and remained the same in <i>B. stacei</i>. A two-way hierarchical cluster analysis from 117 polymorphic SSRs and the traits of the RSA of the <i>Brachypodium</i> spp. genotypes, was performed. <i>Brachypodium</i> genotypes were separated into three groups corresponding to each species. In the second way of the hierarchical clustering association were observed between five RSA variables and SSR markers, which could be useful in the search for genes or QTLs related to RSA characters.https://www.mdpi.com/2073-4395/10/4/518abiotic stress<i>distachyon</i>cereals<i>hybridum</i>molecular markersroot diversity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Juan M. González Jaime Redondo-Pedraza Yolanda Loarce Rifka Hammami Eva Friero Nicolás Jouve |
spellingShingle |
Juan M. González Jaime Redondo-Pedraza Yolanda Loarce Rifka Hammami Eva Friero Nicolás Jouve Molecular Genetic Analysis of Drought Stress Response Traits in <i>Brachypodium</i> spp. Agronomy abiotic stress <i>distachyon</i> cereals <i>hybridum</i> molecular markers root diversity |
author_facet |
Juan M. González Jaime Redondo-Pedraza Yolanda Loarce Rifka Hammami Eva Friero Nicolás Jouve |
author_sort |
Juan M. González |
title |
Molecular Genetic Analysis of Drought Stress Response Traits in <i>Brachypodium</i> spp. |
title_short |
Molecular Genetic Analysis of Drought Stress Response Traits in <i>Brachypodium</i> spp. |
title_full |
Molecular Genetic Analysis of Drought Stress Response Traits in <i>Brachypodium</i> spp. |
title_fullStr |
Molecular Genetic Analysis of Drought Stress Response Traits in <i>Brachypodium</i> spp. |
title_full_unstemmed |
Molecular Genetic Analysis of Drought Stress Response Traits in <i>Brachypodium</i> spp. |
title_sort |
molecular genetic analysis of drought stress response traits in <i>brachypodium</i> spp. |
publisher |
MDPI AG |
series |
Agronomy |
issn |
2073-4395 |
publishDate |
2020-04-01 |
description |
The root is the organ responsible for the uptake of water and therefore has a very important role in drought tolerance. The aims of the present work were to characterize nine traits of the root system architecture (RSA) and the shoot dry weight (W) of twelve genotypes of <i>Brachypodium</i> spp. under water stress and to establish the relationship between RSA phenotyping traits and SSRs. Two culture media, one standard (SM) and one (PEG) to induced water stress have been used. In SM medium, <i>B. stacei</i> had the highest values of W and all the RSA traits, except the mean diameter of the seminal roots, followed by <i>B. hybridum</i> and <i>B. distachyon</i>. In the PEG medium, root length increased in <i>B. distachyon</i>, decreased in <i>B. hybridum</i> and remained the same in <i>B. stacei</i>. A two-way hierarchical cluster analysis from 117 polymorphic SSRs and the traits of the RSA of the <i>Brachypodium</i> spp. genotypes, was performed. <i>Brachypodium</i> genotypes were separated into three groups corresponding to each species. In the second way of the hierarchical clustering association were observed between five RSA variables and SSR markers, which could be useful in the search for genes or QTLs related to RSA characters. |
topic |
abiotic stress <i>distachyon</i> cereals <i>hybridum</i> molecular markers root diversity |
url |
https://www.mdpi.com/2073-4395/10/4/518 |
work_keys_str_mv |
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1721561554864832512 |