Investigation of the interaction between two phenylethanoid glycosides and bovine serum albumin by spectroscopic methods

Investigation of the interaction between two phenylethanoid glycosides and bovine serum albumin by spectroscopic methods

Two phenylethanoid glycosides, acteoside and forsythoside B, were first isolated from the traditional Chinese herb Callicarpa peii H.T. Chang. The interaction between the two phenylethanoid glycosides and bovine serum albumin (BSA) was investigated by fluorescence, UVâvis absorbance and circular dic...

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Bibliographic Details
Main Authors: Ai-Zhi Wu, Chao-Zhan Lin, Ya-Jing Zhai, Jia-Lin Zhuo, Chen-Chen Zhu
Format: Article
Language:English
Published: Elsevier 2013-02-01
Series:Journal of Pharmaceutical Analysis
Online Access:http://www.sciencedirect.com/science/article/pii/S2095177912000846
Description
Summary:Two phenylethanoid glycosides, acteoside and forsythoside B, were first isolated from the traditional Chinese herb Callicarpa peii H.T. Chang. The interaction between the two phenylethanoid glycosides and bovine serum albumin (BSA) was investigated by fluorescence, UVâvis absorbance and circular dichroism (CD). The results showed that the quenching mechanism in the drugâBSA binary systems was a combination of static quenching and non-radiative energy transfer. Displacement experiments confirmed that the drug bound to the site I of BSA. UVâvis and CD measurements indicated that the binding of the drug to BSA induced conformational changes in BSA. Keywords: Phenylethanoid glycosides, BSA, Fluorescence quenching, Site competitive binding
ISSN:2095-1779

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