β-Hemolytic Streptococcus anginosus subsp. anginosus causes streptolysin S-dependent cytotoxicity to human cell culture lines in vitro
Background: Streptococcus anginosus subsp. anginosus (SAA) is one of the opportunistic pathogens in humans that inhabits the oral cavity. The type strain of SAA, NCTC10713T, showed clear β-hemolysis on blood agar plates, and the sole β-hemolytic factor revealed two streptolysin S (SLS) molecules. SL...
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doaj-ef9e7a8c354a4c52848f5d89b1f64a832020-11-25T02:11:04ZengTaylor & Francis GroupJournal of Oral Microbiology2000-22972019-01-0111110.1080/20002297.2019.16098391609839β-Hemolytic Streptococcus anginosus subsp. anginosus causes streptolysin S-dependent cytotoxicity to human cell culture lines in vitroAtsushi Tabata0Takuya Yamada1Hiromi Ohtani2Kazuto Ohkura3Toshifumi Tomoyasu4Hideaki Nagamune5Tokushima University Graduate SchoolTokushima University Graduate SchoolTokushima University Graduate SchoolSuzuka University of Medical ScienceTokushima University Graduate SchoolTokushima University Graduate SchoolBackground: Streptococcus anginosus subsp. anginosus (SAA) is one of the opportunistic pathogens in humans that inhabits the oral cavity. The type strain of SAA, NCTC10713T, showed clear β-hemolysis on blood agar plates, and the sole β-hemolytic factor revealed two streptolysin S (SLS) molecules. SLS is well known as the peptide hemolysin produced from the human pathogen S. pyogenes and shows not only hemolytic activity on erythrocytes but also cytotoxic activity in cell culture lines in vitro and in vivo, such as in a mouse infection model. However, no cytotoxic activity of SLS produced from β-hemolytic SAA (β-SAA) has been reported so far. Objective and Design: In this study, the SLS-dependent cytotoxicity of the β-SAA strains including the genetically modified strains was investigated in vitro. Results: The SLS-producing β-SAA showed cytotoxicity in human cell culture lines under the co-cultivation condition and it was found that this cytotoxicity was caused by the SLS secreted into the extracellular milieu. Conclusion: The results from this study suggest that the SLS produced from β-SAA might indicate the cytotoxic potential similar to that of the SLS from S. pyogenes and the SLS-producing β-SAA would be recognized as “a wolf in sheep’s clothing” More attention will be paid to the pathogenicity of β-hemolytic Anginosus group streptococci.http://dx.doi.org/10.1080/20002297.2019.1609839streptococcus anginosusanginosus group streptococcistreptolysin sslsβ-hemolysiscytotoxicity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Atsushi Tabata Takuya Yamada Hiromi Ohtani Kazuto Ohkura Toshifumi Tomoyasu Hideaki Nagamune |
spellingShingle |
Atsushi Tabata Takuya Yamada Hiromi Ohtani Kazuto Ohkura Toshifumi Tomoyasu Hideaki Nagamune β-Hemolytic Streptococcus anginosus subsp. anginosus causes streptolysin S-dependent cytotoxicity to human cell culture lines in vitro Journal of Oral Microbiology streptococcus anginosus anginosus group streptococci streptolysin s sls β-hemolysis cytotoxicity |
author_facet |
Atsushi Tabata Takuya Yamada Hiromi Ohtani Kazuto Ohkura Toshifumi Tomoyasu Hideaki Nagamune |
author_sort |
Atsushi Tabata |
title |
β-Hemolytic Streptococcus anginosus subsp. anginosus causes streptolysin S-dependent cytotoxicity to human cell culture lines in vitro |
title_short |
β-Hemolytic Streptococcus anginosus subsp. anginosus causes streptolysin S-dependent cytotoxicity to human cell culture lines in vitro |
title_full |
β-Hemolytic Streptococcus anginosus subsp. anginosus causes streptolysin S-dependent cytotoxicity to human cell culture lines in vitro |
title_fullStr |
β-Hemolytic Streptococcus anginosus subsp. anginosus causes streptolysin S-dependent cytotoxicity to human cell culture lines in vitro |
title_full_unstemmed |
β-Hemolytic Streptococcus anginosus subsp. anginosus causes streptolysin S-dependent cytotoxicity to human cell culture lines in vitro |
title_sort |
β-hemolytic streptococcus anginosus subsp. anginosus causes streptolysin s-dependent cytotoxicity to human cell culture lines in vitro |
publisher |
Taylor & Francis Group |
series |
Journal of Oral Microbiology |
issn |
2000-2297 |
publishDate |
2019-01-01 |
description |
Background: Streptococcus anginosus subsp. anginosus (SAA) is one of the opportunistic pathogens in humans that inhabits the oral cavity. The type strain of SAA, NCTC10713T, showed clear β-hemolysis on blood agar plates, and the sole β-hemolytic factor revealed two streptolysin S (SLS) molecules. SLS is well known as the peptide hemolysin produced from the human pathogen S. pyogenes and shows not only hemolytic activity on erythrocytes but also cytotoxic activity in cell culture lines in vitro and in vivo, such as in a mouse infection model. However, no cytotoxic activity of SLS produced from β-hemolytic SAA (β-SAA) has been reported so far. Objective and Design: In this study, the SLS-dependent cytotoxicity of the β-SAA strains including the genetically modified strains was investigated in vitro. Results: The SLS-producing β-SAA showed cytotoxicity in human cell culture lines under the co-cultivation condition and it was found that this cytotoxicity was caused by the SLS secreted into the extracellular milieu. Conclusion: The results from this study suggest that the SLS produced from β-SAA might indicate the cytotoxic potential similar to that of the SLS from S. pyogenes and the SLS-producing β-SAA would be recognized as “a wolf in sheep’s clothing” More attention will be paid to the pathogenicity of β-hemolytic Anginosus group streptococci. |
topic |
streptococcus anginosus anginosus group streptococci streptolysin s sls β-hemolysis cytotoxicity |
url |
http://dx.doi.org/10.1080/20002297.2019.1609839 |
work_keys_str_mv |
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