In Vitro Culture-Induced Pluripotency of Human Spermatogonial Stem Cells
Unipotent spermatogonial stem cells (SSCs) can be transformed into ESC-like cells that exhibit pluripotency in vitro. However, except for mouse models, their characterization and their origins have remained controversies in other models including humans. This controversy has arisen primarily from th...
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doaj-efafebafce03430b8e1128a761d036742020-11-24T23:08:22ZengHindawi LimitedBioMed Research International2314-61332314-61412013-01-01201310.1155/2013/143028143028In Vitro Culture-Induced Pluripotency of Human Spermatogonial Stem CellsJung Jin Lim0Hyung Joon Kim1Kye-Seong Kim2Jae Yup Hong3Dong Ryul Lee4Fertility Center of CHA Gangnam Medical Center, College of Medicine, CHA University, 606-5 Yeoksam-dong, Gangnam-gu, Seoul 135-081, Republic of KoreaFertility Center of CHA Gangnam Medical Center, College of Medicine, CHA University, 606-5 Yeoksam-dong, Gangnam-gu, Seoul 135-081, Republic of KoreaDepartment of Anatomy and Cell Biology, College of Medicine, Hanyang University, Seoul 133-791, Republic of KoreaDepartment of Urology, CHA Bundang Medical Center, CHA University, Seongnam 463-712, Republic of KoreaFertility Center of CHA Gangnam Medical Center, College of Medicine, CHA University, 606-5 Yeoksam-dong, Gangnam-gu, Seoul 135-081, Republic of KoreaUnipotent spermatogonial stem cells (SSCs) can be transformed into ESC-like cells that exhibit pluripotency in vitro. However, except for mouse models, their characterization and their origins have remained controversies in other models including humans. This controversy has arisen primarily from the lack of the direct induction of ESC-like cells from well-characterized SSCs. Thus, the aim of the present study was to find and characterize pluripotent human SSCs in in vitro cultures of characterized SSCs. Human testicular tissues were dissociated and plated onto gelatin/laminin-coated dishes to isolate SSCs. In the presence of growth factors SSCs formed multicellular clumps after 2–4 weeks of culture. At passages 1 and 5, the clumps were dissociated and were then analyzed using markers of pluripotent cells. The number of SSEA-4-positive cells was extremely low but increased gradually up to ~ 10% in the SSC clumps during culture. Most of the SSEA-4-negative cells expressed markers for SSCs, and some cells coexpressed markers of both pluripotent and germ cells. The pluripotent cells formed embryoid bodies and teratomas that contained derivatives of the three germ layers in SCID mice. These results suggest that the pluripotent cells present within the clumps were derived directly from SSCs during in vitro culture.http://dx.doi.org/10.1155/2013/143028 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jung Jin Lim Hyung Joon Kim Kye-Seong Kim Jae Yup Hong Dong Ryul Lee |
spellingShingle |
Jung Jin Lim Hyung Joon Kim Kye-Seong Kim Jae Yup Hong Dong Ryul Lee In Vitro Culture-Induced Pluripotency of Human Spermatogonial Stem Cells BioMed Research International |
author_facet |
Jung Jin Lim Hyung Joon Kim Kye-Seong Kim Jae Yup Hong Dong Ryul Lee |
author_sort |
Jung Jin Lim |
title |
In Vitro Culture-Induced Pluripotency of Human Spermatogonial Stem Cells |
title_short |
In Vitro Culture-Induced Pluripotency of Human Spermatogonial Stem Cells |
title_full |
In Vitro Culture-Induced Pluripotency of Human Spermatogonial Stem Cells |
title_fullStr |
In Vitro Culture-Induced Pluripotency of Human Spermatogonial Stem Cells |
title_full_unstemmed |
In Vitro Culture-Induced Pluripotency of Human Spermatogonial Stem Cells |
title_sort |
in vitro culture-induced pluripotency of human spermatogonial stem cells |
publisher |
Hindawi Limited |
series |
BioMed Research International |
issn |
2314-6133 2314-6141 |
publishDate |
2013-01-01 |
description |
Unipotent spermatogonial stem cells (SSCs) can be transformed into ESC-like cells that exhibit pluripotency in vitro. However, except for mouse models, their characterization and their origins have remained controversies in other models including humans. This controversy has arisen primarily from the lack of the direct induction of ESC-like cells from well-characterized SSCs. Thus, the aim of the present study was to find and characterize pluripotent human SSCs in in vitro cultures of characterized SSCs. Human testicular tissues were dissociated and plated onto gelatin/laminin-coated dishes to isolate SSCs. In the presence of growth factors SSCs formed multicellular clumps after 2–4 weeks of culture. At passages 1 and 5, the clumps were dissociated and were then analyzed using markers of pluripotent cells. The number of SSEA-4-positive cells was extremely low but increased gradually up to ~ 10% in the SSC clumps during culture. Most of the SSEA-4-negative cells expressed markers for SSCs, and some cells coexpressed markers of both pluripotent and germ cells. The pluripotent cells formed embryoid bodies and teratomas that contained derivatives of the three germ layers in SCID mice. These results suggest that the pluripotent cells present within the clumps were derived directly from SSCs during in vitro culture. |
url |
http://dx.doi.org/10.1155/2013/143028 |
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