Genome-Wide Association Mapping for Heat Stress Responsive Traits in Field Pea
Environmental stress hampers pea productivity. To understand the genetic basis of heat resistance, a genome-wide association study (GWAS) was conducted on six stress responsive traits of physiological and agronomic importance in pea, with an objective to identify the genetic loci associated with the...
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doaj-efb260e0f6a54a1ca311356159f74a702020-11-25T02:25:05ZengMDPI AGInternational Journal of Molecular Sciences1422-00672020-03-01216204310.3390/ijms21062043ijms21062043Genome-Wide Association Mapping for Heat Stress Responsive Traits in Field PeaEndale G. Tafesse0Krishna K. Gali1V.B. Reddy Lachagari2Rosalind Bueckert3Thomas D. Warkentin4Department of Plant Sciences, College of Agriculture and Bio-resources, University of Saskatchewan, Saskatoon, SK S7N 5A8, CanadaDepartment of Plant Sciences, College of Agriculture and Bio-resources, University of Saskatchewan, Saskatoon, SK S7N 5A8, CanadaAgriGenome Labs Pvt. Ltd., Hyderabad 500 078, IndiaDepartment of Plant Sciences, College of Agriculture and Bio-resources, University of Saskatchewan, Saskatoon, SK S7N 5A8, CanadaDepartment of Plant Sciences, College of Agriculture and Bio-resources, University of Saskatchewan, Saskatoon, SK S7N 5A8, CanadaEnvironmental stress hampers pea productivity. To understand the genetic basis of heat resistance, a genome-wide association study (GWAS) was conducted on six stress responsive traits of physiological and agronomic importance in pea, with an objective to identify the genetic loci associated with these traits. One hundred and thirty-five genetically diverse pea accessions from major pea growing areas of the world were phenotyped in field trials across five environments, under generally ambient (control) and heat stress conditions. Statistical analysis of phenotype indicated significant effects of genotype (G), environment (E), and G × E interaction for all traits. A total of 16,877 known high-quality SNPs were used for association analysis to determine marker-trait associations (MTA). We identified 32 MTAs that were consistent in at least three environments for association with the traits of stress resistance: six for chlorophyll concentration measured by a soil plant analysis development meter; two each for photochemical reflectance index and canopy temperature; seven for reproductive stem length; six for internode length; and nine for pod number. Forty-eight candidate genes were identified within 15 kb distance of these markers. The identified markers and candidate genes have potential for marker-assisted selection towards the development of heat resistant pea cultivars.https://www.mdpi.com/1422-0067/21/6/2043peaheat stressgenetic diversitygwasgenotyping-by-sequencingmarker-trait associationcandidate-gene |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Endale G. Tafesse Krishna K. Gali V.B. Reddy Lachagari Rosalind Bueckert Thomas D. Warkentin |
spellingShingle |
Endale G. Tafesse Krishna K. Gali V.B. Reddy Lachagari Rosalind Bueckert Thomas D. Warkentin Genome-Wide Association Mapping for Heat Stress Responsive Traits in Field Pea International Journal of Molecular Sciences pea heat stress genetic diversity gwas genotyping-by-sequencing marker-trait association candidate-gene |
author_facet |
Endale G. Tafesse Krishna K. Gali V.B. Reddy Lachagari Rosalind Bueckert Thomas D. Warkentin |
author_sort |
Endale G. Tafesse |
title |
Genome-Wide Association Mapping for Heat Stress Responsive Traits in Field Pea |
title_short |
Genome-Wide Association Mapping for Heat Stress Responsive Traits in Field Pea |
title_full |
Genome-Wide Association Mapping for Heat Stress Responsive Traits in Field Pea |
title_fullStr |
Genome-Wide Association Mapping for Heat Stress Responsive Traits in Field Pea |
title_full_unstemmed |
Genome-Wide Association Mapping for Heat Stress Responsive Traits in Field Pea |
title_sort |
genome-wide association mapping for heat stress responsive traits in field pea |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1422-0067 |
publishDate |
2020-03-01 |
description |
Environmental stress hampers pea productivity. To understand the genetic basis of heat resistance, a genome-wide association study (GWAS) was conducted on six stress responsive traits of physiological and agronomic importance in pea, with an objective to identify the genetic loci associated with these traits. One hundred and thirty-five genetically diverse pea accessions from major pea growing areas of the world were phenotyped in field trials across five environments, under generally ambient (control) and heat stress conditions. Statistical analysis of phenotype indicated significant effects of genotype (G), environment (E), and G × E interaction for all traits. A total of 16,877 known high-quality SNPs were used for association analysis to determine marker-trait associations (MTA). We identified 32 MTAs that were consistent in at least three environments for association with the traits of stress resistance: six for chlorophyll concentration measured by a soil plant analysis development meter; two each for photochemical reflectance index and canopy temperature; seven for reproductive stem length; six for internode length; and nine for pod number. Forty-eight candidate genes were identified within 15 kb distance of these markers. The identified markers and candidate genes have potential for marker-assisted selection towards the development of heat resistant pea cultivars. |
topic |
pea heat stress genetic diversity gwas genotyping-by-sequencing marker-trait association candidate-gene |
url |
https://www.mdpi.com/1422-0067/21/6/2043 |
work_keys_str_mv |
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