Combination of cyclophosphamide and shengbai decoction has synergistic effect against melanoma
Shengbai decoction (SBD), a famous Chinese herbal prescription, has been used for treatment of leukopenia for decades in China. In this study, its synergistic antitumor effect in combination with cyclophosphamide (CTX) on melanoma-bearing mice wasinvestigated. A total of forty C57BL/6 male mice succ...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2020-06-01
|
Series: | Biomedicine & Pharmacotherapy |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S0753332220300561 |
id |
doaj-efbb2a870b5a4ddca51cc36e6aad31b5 |
---|---|
record_format |
Article |
spelling |
doaj-efbb2a870b5a4ddca51cc36e6aad31b52021-05-20T07:40:27ZengElsevierBiomedicine & Pharmacotherapy0753-33222020-06-01126109866Combination of cyclophosphamide and shengbai decoction has synergistic effect against melanomaHuimin Wang0Min Qiu1Yinghui Li2Dandan Cheng3Donghua Yang4Wenpeng Yuan5Heze Branch, Qilu University of Technology (Shandong Academy of Sciences), Biological Engineering Technology Innovation Center of Shandong Province ChinaHeze Branch, Qilu University of Technology (Shandong Academy of Sciences), Biological Engineering Technology Innovation Center of Shandong Province ChinaHeze Branch, Qilu University of Technology (Shandong Academy of Sciences), Biological Engineering Technology Innovation Center of Shandong Province ChinaHeze Branch, Qilu University of Technology (Shandong Academy of Sciences), Biological Engineering Technology Innovation Center of Shandong Province ChinaDepartment of Pharmacology College of Pharmacy and Health Sciences St. John's University United StatesHeze Branch, Qilu University of Technology (Shandong Academy of Sciences), Biological Engineering Technology Innovation Center of Shandong Province China; Corresponding author.Shengbai decoction (SBD), a famous Chinese herbal prescription, has been used for treatment of leukopenia for decades in China. In this study, its synergistic antitumor effect in combination with cyclophosphamide (CTX) on melanoma-bearing mice wasinvestigated. A total of forty C57BL/6 male mice successfully modeled (6–8 weeks old, 18−22 g) were randomly divided into 4 groups (n = 10): 1) the model group, 2) the CTX group, 3) the low dose of SBD (10.66 g/kg/d, raw medicine) and CTX group, and 4) the high dose of SBD (31.98 g/kg/d, raw medicine) and CTX group. Melanoma mice models were established by injection of 0.1 mL of melanoma cell suspension under the midline of the back of each C57BL/6 mouse. Treatment started five days after modeling. The results showed that SBD significantly alleviated histopathological damage, and reduced tumor growth and the concentrations of IL-6, IFN-γ and TNF-α in serum. Furthermore, the combined therapy increased the positive expression of NF-κB and promoted apoptosis compared with CTX alone. These results indicated that SBD could improve the antitumor effect of CTX on melanoma in vivo. And this combination treatment may be an ideal therapy against melanoma.http://www.sciencedirect.com/science/article/pii/S0753332220300561Shengbai decoctionCyclophosphamideSynergistic anti-tumorMelanoma |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Huimin Wang Min Qiu Yinghui Li Dandan Cheng Donghua Yang Wenpeng Yuan |
spellingShingle |
Huimin Wang Min Qiu Yinghui Li Dandan Cheng Donghua Yang Wenpeng Yuan Combination of cyclophosphamide and shengbai decoction has synergistic effect against melanoma Biomedicine & Pharmacotherapy Shengbai decoction Cyclophosphamide Synergistic anti-tumor Melanoma |
author_facet |
Huimin Wang Min Qiu Yinghui Li Dandan Cheng Donghua Yang Wenpeng Yuan |
author_sort |
Huimin Wang |
title |
Combination of cyclophosphamide and shengbai decoction has synergistic effect against melanoma |
title_short |
Combination of cyclophosphamide and shengbai decoction has synergistic effect against melanoma |
title_full |
Combination of cyclophosphamide and shengbai decoction has synergistic effect against melanoma |
title_fullStr |
Combination of cyclophosphamide and shengbai decoction has synergistic effect against melanoma |
title_full_unstemmed |
Combination of cyclophosphamide and shengbai decoction has synergistic effect against melanoma |
title_sort |
combination of cyclophosphamide and shengbai decoction has synergistic effect against melanoma |
publisher |
Elsevier |
series |
Biomedicine & Pharmacotherapy |
issn |
0753-3322 |
publishDate |
2020-06-01 |
description |
Shengbai decoction (SBD), a famous Chinese herbal prescription, has been used for treatment of leukopenia for decades in China. In this study, its synergistic antitumor effect in combination with cyclophosphamide (CTX) on melanoma-bearing mice wasinvestigated. A total of forty C57BL/6 male mice successfully modeled (6–8 weeks old, 18−22 g) were randomly divided into 4 groups (n = 10): 1) the model group, 2) the CTX group, 3) the low dose of SBD (10.66 g/kg/d, raw medicine) and CTX group, and 4) the high dose of SBD (31.98 g/kg/d, raw medicine) and CTX group. Melanoma mice models were established by injection of 0.1 mL of melanoma cell suspension under the midline of the back of each C57BL/6 mouse. Treatment started five days after modeling. The results showed that SBD significantly alleviated histopathological damage, and reduced tumor growth and the concentrations of IL-6, IFN-γ and TNF-α in serum. Furthermore, the combined therapy increased the positive expression of NF-κB and promoted apoptosis compared with CTX alone. These results indicated that SBD could improve the antitumor effect of CTX on melanoma in vivo. And this combination treatment may be an ideal therapy against melanoma. |
topic |
Shengbai decoction Cyclophosphamide Synergistic anti-tumor Melanoma |
url |
http://www.sciencedirect.com/science/article/pii/S0753332220300561 |
work_keys_str_mv |
AT huiminwang combinationofcyclophosphamideandshengbaidecoctionhassynergisticeffectagainstmelanoma AT minqiu combinationofcyclophosphamideandshengbaidecoctionhassynergisticeffectagainstmelanoma AT yinghuili combinationofcyclophosphamideandshengbaidecoctionhassynergisticeffectagainstmelanoma AT dandancheng combinationofcyclophosphamideandshengbaidecoctionhassynergisticeffectagainstmelanoma AT donghuayang combinationofcyclophosphamideandshengbaidecoctionhassynergisticeffectagainstmelanoma AT wenpengyuan combinationofcyclophosphamideandshengbaidecoctionhassynergisticeffectagainstmelanoma |
_version_ |
1721435166868504576 |