A Fluorescence‐Based Assay System for the Determination of Haloperoxidase‐Activity Using a Two‐Dimensional Calibration Ap‐proach

A Fluorescence‐Based Assay System for the Determination of Haloperoxidase‐Activity Using a Two‐Dimensional Calibration Ap‐proach

Abstract Screening for an interesting biocatalyst and its subsequent kinetic characterization depends on a reliable activity assay. In this work, a fluorometric assay based on the halogenation of 4‐methyl‐7‐diethylamino‐coumarin was established to monitor haloperoxidase‐activity. Since haloperoxidas...

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Bibliographic Details
Main Authors: Alexander V. Fejzagić, Sebastian Myllek, Fabian Hogenkamp, Julian Greb, Prof. Dr. Jörg Pietruszka, Dr. Thomas Classen
Format: Article
Language:English
Published: Wiley-VCH 2020-09-01
Series:ChemistryOpen
Subjects:
halogenation
fluorescence spectroscopy
kinetics
enzyme catalysis
hypobromite
Online Access:https://doi.org/10.1002/open.202000184

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