Pharmacological activity and quantitative analysis of flavonoids isolated from the flowers of Begonia semperflorens Link et Otto
Abstract Begonia semperflorens Link et Otto has been broadly raised up for ornamental purpose as well comestible blossom. As the reproductive structures of phanerogams, flowers contain various secondary metabolites and have many biological activities. Accordingly, we began the contrivance for isolat...
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doaj-f000b322119b45eaa19710a9fa7d87362020-11-24T21:24:30ZengSpringerOpenApplied Biological Chemistry2468-08342468-08422019-03-016211810.1186/s13765-019-0416-6Pharmacological activity and quantitative analysis of flavonoids isolated from the flowers of Begonia semperflorens Link et OttoJung-Hwa Kwon0Hyun-Ji Oh1Dong-Sung Lee2Seo-Ji In3Kyeong-Hwa Seo4Jae-Woo Jung5Byeong-Ju Cha6Dae Young Lee7Nam-In Baek8Department of Oriental Medicine Biotechnology, Graduate School of Biotechnology, Kyung Hee UniversityDepartment of Oriental Medicine Biotechnology, Graduate School of Biotechnology, Kyung Hee UniversityChosun UniversityDepartment of Oriental Medicine Biotechnology, Graduate School of Biotechnology, Kyung Hee UniversityBiological and Genetic Resources Utilization Division, National Institute of Biological ResourcesDepartment of Oriental Medicine Biotechnology, Graduate School of Biotechnology, Kyung Hee UniversityDepartment of Oriental Medicine Biotechnology, Graduate School of Biotechnology, Kyung Hee UniversityDepartment Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDADepartment of Oriental Medicine Biotechnology, Graduate School of Biotechnology, Kyung Hee UniversityAbstract Begonia semperflorens Link et Otto has been broadly raised up for ornamental purpose as well comestible blossom. As the reproductive structures of phanerogams, flowers contain various secondary metabolites and have many biological activities. Accordingly, we began the contrivance for isolation and analysis of flavonoids contained in B. semperflorens flowers. MeOH extraction of B. semperflorens followed solvent fractionation was prosecuted. Column chromatography of non-polar fraction gave four flavonoids using several resins. Identification of the flavonols were established as quercetin (1), kaempferol (2), astragalin (3), and isoquercetin (4) by interpreting a variety of spectral information. Quercetin (1) and kaempferol (2) inhibited NO production and protected against t-BHP-induced oxidative stress. Kaempferol (2) also protected cell death of glutamate-treated HT22. Quantitative analysis of flavonoid content in B. semperflorens flowers was also performed using HPLC experiment.http://link.springer.com/article/10.1186/s13765-019-0416-6Begonia semperflorensHepatoprotectiveKaempferolNeuroprotectiveNitric oxideQuercetin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jung-Hwa Kwon Hyun-Ji Oh Dong-Sung Lee Seo-Ji In Kyeong-Hwa Seo Jae-Woo Jung Byeong-Ju Cha Dae Young Lee Nam-In Baek |
spellingShingle |
Jung-Hwa Kwon Hyun-Ji Oh Dong-Sung Lee Seo-Ji In Kyeong-Hwa Seo Jae-Woo Jung Byeong-Ju Cha Dae Young Lee Nam-In Baek Pharmacological activity and quantitative analysis of flavonoids isolated from the flowers of Begonia semperflorens Link et Otto Applied Biological Chemistry Begonia semperflorens Hepatoprotective Kaempferol Neuroprotective Nitric oxide Quercetin |
author_facet |
Jung-Hwa Kwon Hyun-Ji Oh Dong-Sung Lee Seo-Ji In Kyeong-Hwa Seo Jae-Woo Jung Byeong-Ju Cha Dae Young Lee Nam-In Baek |
author_sort |
Jung-Hwa Kwon |
title |
Pharmacological activity and quantitative analysis of flavonoids isolated from the flowers of Begonia semperflorens Link et Otto |
title_short |
Pharmacological activity and quantitative analysis of flavonoids isolated from the flowers of Begonia semperflorens Link et Otto |
title_full |
Pharmacological activity and quantitative analysis of flavonoids isolated from the flowers of Begonia semperflorens Link et Otto |
title_fullStr |
Pharmacological activity and quantitative analysis of flavonoids isolated from the flowers of Begonia semperflorens Link et Otto |
title_full_unstemmed |
Pharmacological activity and quantitative analysis of flavonoids isolated from the flowers of Begonia semperflorens Link et Otto |
title_sort |
pharmacological activity and quantitative analysis of flavonoids isolated from the flowers of begonia semperflorens link et otto |
publisher |
SpringerOpen |
series |
Applied Biological Chemistry |
issn |
2468-0834 2468-0842 |
publishDate |
2019-03-01 |
description |
Abstract Begonia semperflorens Link et Otto has been broadly raised up for ornamental purpose as well comestible blossom. As the reproductive structures of phanerogams, flowers contain various secondary metabolites and have many biological activities. Accordingly, we began the contrivance for isolation and analysis of flavonoids contained in B. semperflorens flowers. MeOH extraction of B. semperflorens followed solvent fractionation was prosecuted. Column chromatography of non-polar fraction gave four flavonoids using several resins. Identification of the flavonols were established as quercetin (1), kaempferol (2), astragalin (3), and isoquercetin (4) by interpreting a variety of spectral information. Quercetin (1) and kaempferol (2) inhibited NO production and protected against t-BHP-induced oxidative stress. Kaempferol (2) also protected cell death of glutamate-treated HT22. Quantitative analysis of flavonoid content in B. semperflorens flowers was also performed using HPLC experiment. |
topic |
Begonia semperflorens Hepatoprotective Kaempferol Neuroprotective Nitric oxide Quercetin |
url |
http://link.springer.com/article/10.1186/s13765-019-0416-6 |
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