Differential Tick Salivary Protein Profiles and Human Immune Responses to Lone Star Ticks (Amblyomma americanum) From the Wild vs. a Laboratory Colony
Ticks are a growing concern to human and animal health worldwide and they are leading vectors of arthropod-borne pathogens in the United States. Ticks are pool blood feeders that can attach to the host skin for days to weeks using their saliva to counteract the host defenses. Tick saliva, as in othe...
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doaj-f00f9245b1774ff098f7df810c248a962020-11-25T01:08:14ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-08-011010.3389/fimmu.2019.01996466646Differential Tick Salivary Protein Profiles and Human Immune Responses to Lone Star Ticks (Amblyomma americanum) From the Wild vs. a Laboratory ColonyL. Paulina Maldonado-Ruiz0Lidia Montenegro-Cadena1Brittany Blattner2Sapna Menghwar3Ludek Zurek4Berlin Londono-Renteria5Medical/Veterinary Entomology Laboratory, Department of Entomology, Kansas State University, Manhattan, KS, United StatesVector Biology Laboratory, Department of Entomology, Kansas State University, Manhattan, KS, United StatesVector Biology Laboratory, Department of Entomology, Kansas State University, Manhattan, KS, United StatesVector Biology Laboratory, Department of Entomology, Kansas State University, Manhattan, KS, United StatesDepartment of Pathology and Parasitology, CEITEC Center for Zoonoses, University of Veterinary and Pharmaceutical Sciences, Brno, CzechiaVector Biology Laboratory, Department of Entomology, Kansas State University, Manhattan, KS, United StatesTicks are a growing concern to human and animal health worldwide and they are leading vectors of arthropod-borne pathogens in the United States. Ticks are pool blood feeders that can attach to the host skin for days to weeks using their saliva to counteract the host defenses. Tick saliva, as in other hematophagous arthropods, contains pharmacological and immunological active compounds, which modulate local and systemic immune responses and induce antibody production. In the present study, we explore differences in the salivary gland extract (SGE) protein content of Amblyomma americanum ticks raised in a laboratory colony (CT) vs. those collected in the field (FT). First, we measured the IgG antibody levels against SGE in healthy volunteers residing in Kansas. ELISA test showed higher IgG antibody levels when using the SGE from CT as antigen. Interestingly, antibody levels against both, CT-SGE and FT-SGE, were high in the warm months (May–June) and decreased in the cold months (September–November). Immunoblot testing revealed a set of different immunogenic bands for each group of ticks and mass spectrometry data revealed differences in at 19 proteins specifically identified in the CT-SGE group and 20 from the FT-SGE group. Our results suggest that differences in the salivary proteins between CT-SGE and FT-SGE may explain the differential immune responses observed in this study.https://www.frontiersin.org/article/10.3389/fimmu.2019.01996/fullAmblyomma americanumlone star ticksalivary proteinshuman immune responseantibodiesseasonal response |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
L. Paulina Maldonado-Ruiz Lidia Montenegro-Cadena Brittany Blattner Sapna Menghwar Ludek Zurek Berlin Londono-Renteria |
spellingShingle |
L. Paulina Maldonado-Ruiz Lidia Montenegro-Cadena Brittany Blattner Sapna Menghwar Ludek Zurek Berlin Londono-Renteria Differential Tick Salivary Protein Profiles and Human Immune Responses to Lone Star Ticks (Amblyomma americanum) From the Wild vs. a Laboratory Colony Frontiers in Immunology Amblyomma americanum lone star tick salivary proteins human immune response antibodies seasonal response |
author_facet |
L. Paulina Maldonado-Ruiz Lidia Montenegro-Cadena Brittany Blattner Sapna Menghwar Ludek Zurek Berlin Londono-Renteria |
author_sort |
L. Paulina Maldonado-Ruiz |
title |
Differential Tick Salivary Protein Profiles and Human Immune Responses to Lone Star Ticks (Amblyomma americanum) From the Wild vs. a Laboratory Colony |
title_short |
Differential Tick Salivary Protein Profiles and Human Immune Responses to Lone Star Ticks (Amblyomma americanum) From the Wild vs. a Laboratory Colony |
title_full |
Differential Tick Salivary Protein Profiles and Human Immune Responses to Lone Star Ticks (Amblyomma americanum) From the Wild vs. a Laboratory Colony |
title_fullStr |
Differential Tick Salivary Protein Profiles and Human Immune Responses to Lone Star Ticks (Amblyomma americanum) From the Wild vs. a Laboratory Colony |
title_full_unstemmed |
Differential Tick Salivary Protein Profiles and Human Immune Responses to Lone Star Ticks (Amblyomma americanum) From the Wild vs. a Laboratory Colony |
title_sort |
differential tick salivary protein profiles and human immune responses to lone star ticks (amblyomma americanum) from the wild vs. a laboratory colony |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2019-08-01 |
description |
Ticks are a growing concern to human and animal health worldwide and they are leading vectors of arthropod-borne pathogens in the United States. Ticks are pool blood feeders that can attach to the host skin for days to weeks using their saliva to counteract the host defenses. Tick saliva, as in other hematophagous arthropods, contains pharmacological and immunological active compounds, which modulate local and systemic immune responses and induce antibody production. In the present study, we explore differences in the salivary gland extract (SGE) protein content of Amblyomma americanum ticks raised in a laboratory colony (CT) vs. those collected in the field (FT). First, we measured the IgG antibody levels against SGE in healthy volunteers residing in Kansas. ELISA test showed higher IgG antibody levels when using the SGE from CT as antigen. Interestingly, antibody levels against both, CT-SGE and FT-SGE, were high in the warm months (May–June) and decreased in the cold months (September–November). Immunoblot testing revealed a set of different immunogenic bands for each group of ticks and mass spectrometry data revealed differences in at 19 proteins specifically identified in the CT-SGE group and 20 from the FT-SGE group. Our results suggest that differences in the salivary proteins between CT-SGE and FT-SGE may explain the differential immune responses observed in this study. |
topic |
Amblyomma americanum lone star tick salivary proteins human immune response antibodies seasonal response |
url |
https://www.frontiersin.org/article/10.3389/fimmu.2019.01996/full |
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