First report on embryonic and larval development of 2n/3n mosaic sterlet
Mosaicism is frequently observed in aquaculture practices, and it adversely affects the production as well as the restoration programme of the sturgeon. The purpose of the present study was the induction of 2n/3n mosaic in sterlet, Acipenser ruthenus L., and compare their embryonic and larval develo...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Elsevier
2021-09-01
|
Series: | Animal |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S1751731121001609 |
id |
doaj-f010663ae36b484c8e0ebbd14342e1a9 |
---|---|
record_format |
Article |
spelling |
doaj-f010663ae36b484c8e0ebbd14342e1a92021-07-31T04:38:05ZengElsevierAnimal1751-73112021-09-01159100317First report on embryonic and larval development of 2n/3n mosaic sterletD. Fopp-Bayat0A. Nitkiewicz1G. Chandra2Corresponding author.; Department of Ichthyology and Aquaculture, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, PolandDepartment of Ichthyology and Aquaculture, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, PolandDepartment of Ichthyology and Aquaculture, Faculty of Animal Bioengineering, University of Warmia and Mazury in Olsztyn, 10-718 Olsztyn, PolandMosaicism is frequently observed in aquaculture practices, and it adversely affects the production as well as the restoration programme of the sturgeon. The purpose of the present study was the induction of 2n/3n mosaic in sterlet, Acipenser ruthenus L., and compare their embryonic and larval development with diploid control sterlet. Microsatellite DNA loci genotyping was conducted for the identification of the genotypes and parentage analysis. Embryonic development was monitored in experimental groups at every 24 h interval. Identification of individual stages of embryonic development was recorded based on a 36-degree scale of development. Additionally, the BW and body length (LT) of experimental fishes were taken during 110 days of the rearing period. The Fulton’s condition coefficient (F), length-weight parameters, and specific growth rate (SGR) coefficient were calculated. The analysis of embryonic development of the 2n/3n mosaic and the diploid control group did not show differences. However, higher mortality (88%) was observed in 2n/3n mosaic groups in comparison to the diploid control groups (55%). BW and body length of 2n/3n mosaic sterlet were slightly lower than the diploid control sterlet, but the differences were not statistically significant. F analysis did not confirm a lower growth performance of the fishes in the 2n/3n mosaic group. Microsatellite DNA loci genotyping confirmed both the incidence of polyspermy and retention of the second polar body. This paper presents the first report on embryonic development and growth performance of 2n/3n mosaic sturgeons.http://www.sciencedirect.com/science/article/pii/S1751731121001609Cold-shockGrowth performanceMicrosatellite genotypingPolyploidizationSturgeon |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
D. Fopp-Bayat A. Nitkiewicz G. Chandra |
spellingShingle |
D. Fopp-Bayat A. Nitkiewicz G. Chandra First report on embryonic and larval development of 2n/3n mosaic sterlet Animal Cold-shock Growth performance Microsatellite genotyping Polyploidization Sturgeon |
author_facet |
D. Fopp-Bayat A. Nitkiewicz G. Chandra |
author_sort |
D. Fopp-Bayat |
title |
First report on embryonic and larval development of 2n/3n mosaic sterlet |
title_short |
First report on embryonic and larval development of 2n/3n mosaic sterlet |
title_full |
First report on embryonic and larval development of 2n/3n mosaic sterlet |
title_fullStr |
First report on embryonic and larval development of 2n/3n mosaic sterlet |
title_full_unstemmed |
First report on embryonic and larval development of 2n/3n mosaic sterlet |
title_sort |
first report on embryonic and larval development of 2n/3n mosaic sterlet |
publisher |
Elsevier |
series |
Animal |
issn |
1751-7311 |
publishDate |
2021-09-01 |
description |
Mosaicism is frequently observed in aquaculture practices, and it adversely affects the production as well as the restoration programme of the sturgeon. The purpose of the present study was the induction of 2n/3n mosaic in sterlet, Acipenser ruthenus L., and compare their embryonic and larval development with diploid control sterlet. Microsatellite DNA loci genotyping was conducted for the identification of the genotypes and parentage analysis. Embryonic development was monitored in experimental groups at every 24 h interval. Identification of individual stages of embryonic development was recorded based on a 36-degree scale of development. Additionally, the BW and body length (LT) of experimental fishes were taken during 110 days of the rearing period. The Fulton’s condition coefficient (F), length-weight parameters, and specific growth rate (SGR) coefficient were calculated. The analysis of embryonic development of the 2n/3n mosaic and the diploid control group did not show differences. However, higher mortality (88%) was observed in 2n/3n mosaic groups in comparison to the diploid control groups (55%). BW and body length of 2n/3n mosaic sterlet were slightly lower than the diploid control sterlet, but the differences were not statistically significant. F analysis did not confirm a lower growth performance of the fishes in the 2n/3n mosaic group. Microsatellite DNA loci genotyping confirmed both the incidence of polyspermy and retention of the second polar body. This paper presents the first report on embryonic development and growth performance of 2n/3n mosaic sturgeons. |
topic |
Cold-shock Growth performance Microsatellite genotyping Polyploidization Sturgeon |
url |
http://www.sciencedirect.com/science/article/pii/S1751731121001609 |
work_keys_str_mv |
AT dfoppbayat firstreportonembryonicandlarvaldevelopmentof2n3nmosaicsterlet AT anitkiewicz firstreportonembryonicandlarvaldevelopmentof2n3nmosaicsterlet AT gchandra firstreportonembryonicandlarvaldevelopmentof2n3nmosaicsterlet |
_version_ |
1721247195553857536 |