A Patient-Derived Xenograft Model of Glioblastoma
Summary: Glioblastoma (GBM) remains the most common malignant primary brain tumor in adults with a median survival of less than ~15 months. Further understanding and therapeutic development rely on the use of clinically relevant models of GBM. Here, we present our patient-derived in vitro and in viv...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-12-01
|
| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166720301660 |
| id |
doaj-f04cc8d3234044c9bf5bf5b44a88dbfc |
|---|---|
| record_format |
Article |
| spelling |
doaj-f04cc8d3234044c9bf5bf5b44a88dbfc2020-12-21T04:49:02ZengElsevierSTAR Protocols2666-16672020-12-0113100179A Patient-Derived Xenograft Model of GlioblastomaChirayu R. Chokshi0Neil Savage1Chitra Venugopal2Sheila K. Singh3Department of Biochemistry, McMaster University, Hamilton, ON L8S 4L8, CanadaDepartment of Biochemistry, McMaster University, Hamilton, ON L8S 4L8, CanadaDepartment of Surgery, McMaster University, Hamilton, ON L8S 4L8, CanadaDepartment of Biochemistry, McMaster University, Hamilton, ON L8S 4L8, Canada; Department of Surgery, McMaster University, Hamilton, ON L8S 4L8, Canada; Corresponding authorSummary: Glioblastoma (GBM) remains the most common malignant primary brain tumor in adults with a median survival of less than ~15 months. Further understanding and therapeutic development rely on the use of clinically relevant models of GBM. Here, we present our patient-derived in vitro and in vivo models that enrich for GBM stem cells (GSCs), a subpopulation of tumor cells with stem cell-like properties that recapitulate the cellular heterogeneity of its parental tumor and resist conventional therapy and seed disease relapse.For complete details on the use and execution of this protocol, please refer to Vora et al. (2020).http://www.sciencedirect.com/science/article/pii/S2666166720301660Cell cultureCell isolationCancerModel Organisms |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Chirayu R. Chokshi Neil Savage Chitra Venugopal Sheila K. Singh |
| spellingShingle |
Chirayu R. Chokshi Neil Savage Chitra Venugopal Sheila K. Singh A Patient-Derived Xenograft Model of Glioblastoma STAR Protocols Cell culture Cell isolation Cancer Model Organisms |
| author_facet |
Chirayu R. Chokshi Neil Savage Chitra Venugopal Sheila K. Singh |
| author_sort |
Chirayu R. Chokshi |
| title |
A Patient-Derived Xenograft Model of Glioblastoma |
| title_short |
A Patient-Derived Xenograft Model of Glioblastoma |
| title_full |
A Patient-Derived Xenograft Model of Glioblastoma |
| title_fullStr |
A Patient-Derived Xenograft Model of Glioblastoma |
| title_full_unstemmed |
A Patient-Derived Xenograft Model of Glioblastoma |
| title_sort |
patient-derived xenograft model of glioblastoma |
| publisher |
Elsevier |
| series |
STAR Protocols |
| issn |
2666-1667 |
| publishDate |
2020-12-01 |
| description |
Summary: Glioblastoma (GBM) remains the most common malignant primary brain tumor in adults with a median survival of less than ~15 months. Further understanding and therapeutic development rely on the use of clinically relevant models of GBM. Here, we present our patient-derived in vitro and in vivo models that enrich for GBM stem cells (GSCs), a subpopulation of tumor cells with stem cell-like properties that recapitulate the cellular heterogeneity of its parental tumor and resist conventional therapy and seed disease relapse.For complete details on the use and execution of this protocol, please refer to Vora et al. (2020). |
| topic |
Cell culture Cell isolation Cancer Model Organisms |
| url |
http://www.sciencedirect.com/science/article/pii/S2666166720301660 |
| work_keys_str_mv |
AT chirayurchokshi apatientderivedxenograftmodelofglioblastoma AT neilsavage apatientderivedxenograftmodelofglioblastoma AT chitravenugopal apatientderivedxenograftmodelofglioblastoma AT sheilaksingh apatientderivedxenograftmodelofglioblastoma AT chirayurchokshi patientderivedxenograftmodelofglioblastoma AT neilsavage patientderivedxenograftmodelofglioblastoma AT chitravenugopal patientderivedxenograftmodelofglioblastoma AT sheilaksingh patientderivedxenograftmodelofglioblastoma |
| _version_ |
1724375538197331968 |